ETV5 (NM_004454) Human Tagged ORF Clone

CAT#: RG200366

ETV5 (tGFP-tagged) - Human ets variant 5 (ETV5)

Product Images

Circular map for RG200366

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ETV5
• Synonyms: ERM
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200366 representing NM_004454
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGGGTTTTATGATCAGCAAGTCCCTTTTATGGTCCCAGGGAAATCTCGATCTGAGGAATGCAGAG
  GGCGGCCTGTGATTGACAGAAAGAGGAAGTTTTTGGACACAGATCTGGCTCACGATTCTGAAGAGCTATT
  TCAGGATCTCAGTCAACTTCAAGAGGCTTGGTTAGCTGAAGCACAAGTTCCTGATGATGAACAGTTTGTC
  CCAGATTTTCAGTCTGATAACCTGGTGCTTCATGCCCCACCTCCAACCAAGATCAAACGGGAGCTGCACA
  GCCCCTCCTCTGAGCTGTCGTCTTGTAGCCATGAGCAGGCTCTTGGTGCTAACTATGGAGAAAAGTGCCT
  CTACAACTATTGTGCCTATGATAGGAAGCCTCCCTCTGGGTTCAAGCCATTAACCCCTCCTACAACCCCC
  CTCTCACCCACCCATCAGAATCCCCTATTTCCCCCACCTCAGGCAACTCTGCCCACCTCAGGGCATGCCC
  CTGCAGCTGGCCCAGTTCAAGGTGTGGGCCCCGCCCCCGCCCCCCATTCGCTTCCAGAGCCTGGACCACA
  GCAGCAAACATTTGCGGTCCCCCGACCACCACATCAGCCCCTGCAGATGCCAAAGATGATGCCTGAAAAC
  CAGTATCCATCAGAACAGAGATTTCAGAGACAACTGTCTGAACCCTGCCACCCCTTCCCTCCTCAGCCAG
  GAGTTCCTGGAGATAATCGCCCCAGTTACCATCGGCAAATGTCAGAACCTATTGTCCCTGCAGCTCCCCC
  GCCCCCTCAGGGATTCAAACAAGAATACCATGACCCACTCTATGAACATGGGGTCCCGGGCATGCCAGGG
  CCCCCAGCACACGGGTTCCAGTCACCAATGGGAATCAAGCAGGAGCCTCGGGATTACTGCGTCGATTCAG
  AAGTGCCTAACTGCCAGTCATCCTACATGAGAGGGGGTTATTTCTCCAGCAGCCATGAAGGTTTTTCATA
  TGAAAAAGATCCCCGATTATACTTTGACGACACTTGTGTTGTGCCTGAGAGACTGGAAGGCAAAGTCAAA
  CAGGAGCCTACCATGTATCGAGAGGGGCCCCCTTACCAGAGGCGAGGTTCCCTTCAGCTGTGGCAGTTCC
  TGGTCACCCTTCTTGATGACCCAGCCAATGCCCACTTCATTGCCTGGACAGGTCGAGGCATGGAGTTCAA
  GCTGATAGAACCGGAAGAGGTTGCTCGGCGCTGGGGCATCCAGAAGAACCGGCCAGCCATGAACTATGAC
  AAGCTGAGCCGCTCTCTCCGCTATTACTATGAAAAGGGCATCATGCAGAAGGTGGCTGGAGAGCGATACG
  TCTACAAATTTGTCTGTGACCCAGATGCCCTCTTCTCCATGGCTTTCCCGGATAACCAGCGTCCGTTCCT
  GAAGGCAGAGTCCGAGTGCCACCTCAGCGAGGAGGACACCCTGCCGCTGACCCACTTTGAAGACAGCCCC
  GCTTACCTCCTGGACATGGACCGCTGCAGCAGCCTCCCCTATGCCGAAGGCTTTGCTTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200366 representing NM_004454
  Red=Cloning site Green=Tags(s)
  
  MDGFYDQQVPFMVPGKSRSEECRGRPVIDRKRKFLDTDLAHDSEELFQDLSQLQEAWLAEAQVPDDEQFV
  PDFQSDNLVLHAPPPTKIKRELHSPSSELSSCSHEQALGANYGEKCLYNYCAYDRKPPSGFKPLTPPTTP
  LSPTHQNPLFPPPQATLPTSGHAPAAGPVQGVGPAPAPHSLPEPGPQQQTFAVPRPPHQPLQMPKMMPEN
  QYPSEQRFQRQLSEPCHPFPPQPGVPGDNRPSYHRQMSEPIVPAAPPPPQGFKQEYHDPLYEHGVPGMPG
  PPAHGFQSPMGIKQEPRDYCVDSEVPNCQSSYMRGGYFSSSHEGFSYEKDPRLYFDDTCVVPERLEGKVK
  QEPTMYREGPPYQRRGSLQLWQFLVTLLDDPANAHFIAWTGRGMEFKLIEPEEVARRWGIQKNRPAMNYD
  KLSRSLRYYYEKGIMQKVAGERYVYKFVCDPDALFSMAFPDNQRPFLKAESECHLSEEDTLPLTHFEDSP
  AYLLDMDRCSSLPYAEGFAY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004454
• ORF Size: 1530 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004454.3
• RefSeq Size: 4071 bp
• RefSeq ORF: 1533 bp
• Locus ID: 2119
• UniProt ID: P41161
• Cytogenetics: 3q27.2
• Domains: ETS, ETS_PEA3_N
• Protein Families: Transcription Factors
• Gene Summary: Binds to DNA sequences containing the consensus nucleotide core sequence 5'-GGAA.-3'.[UniProtKB/Swiss-Prot Function]