Annexin A10 (ANXA10) (NM_007193) Human Tagged ORF Clone

CAT#: RG200268

ANXA10 (tGFP-tagged) - Human annexin A10 (ANXA10)

Product Images

Circular map for RG200268

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Annexin A10
• Synonyms: ANX14
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200268 representing NM_007193
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTTGTGGAGACTATGTGCAAGGAACCATCTTCCCAGCTCCCAATTTCAATCCCATAATGGATGCCC
  AAATGCTAGGAGGAGCACTCCAAGGATTTGACTGTGACAAAGACATGCTGATCAACATTCTGACTCAGCG
  CTGCAATGCACAAAGGATGATGATTGCAGAGGCATACCAGAGCATGTATGGCCGGGACCTGATTGGGGAT
  ATGAGGGAGCAGCTTTCGGATCACTTCAAAGATGTGATGGCTGGCCTCATGTACCCACCACCACTGTATG
  ATGCTCATGAGCTCTGGCATGCCATGAAGGGAGTAGGCACTGATGAGAATTGCCTCATTGAAATACTAGC
  TTCAAGAACAAATGGAGAAATTTTCCAGATGCGAGAAGCCTACTGCTTGCAATACAGCAATAACCTCCAA
  GAGGACATTTATTCAGAGACCTCAGGACACTTCAGAGATACTCTCATGAACTTGGTCCAGGGGACCAGAG
  AGGAAGGATATACAGACCCTGCGATGGCTGCTCAGGATGCAATGGTCCTATGGGAAGCCTGTCAGCAGAA
  GACGGGGGAGCACAAAACCATGCTGCAAATGATCCTGTGCAACAAGAGCTACCAGCAGCTGCGGCTGGTT
  TTCCAGGAATTTCAAAATATTTCTGGGCAAGATATGGTAGATGCCATTAATGAATGTTATGATGGATACT
  TTCAGGAGCTGCTGGTTGCAATTGTTCTCTGTGTTCGAGACAAACCAGCCTATTTTGCTTATAGATTATA
  TAGTGCAATTCATGACTTTGGTTTCCATAATAAAACTGTAATCAGGATTCTCATTGCCAGAAGTGAAATA
  GACCTGCTGACCATAAGGAAACGATACAAAGAGCGATATGGAAAATCCCTATTTCATGATATCAGAAATT
  TTGCTTCAGGGCATTATAAGAAAGCACTGCTTGCCATCTGTGCTGGTGATGCTGAGGACTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200268 representing NM_007193
  Red=Cloning site Green=Tags(s)
  
  MFCGDYVQGTIFPAPNFNPIMDAQMLGGALQGFDCDKDMLINILTQRCNAQRMMIAEAYQSMYGRDLIGD
  MREQLSDHFKDVMAGLMYPPPLYDAHELWHAMKGVGTDENCLIEILASRTNGEIFQMREAYCLQYSNNLQ
  EDIYSETSGHFRDTLMNLVQGTREEGYTDPAMAAQDAMVLWEACQQKTGEHKTMLQMILCNKSYQQLRLV
  FQEFQNISGQDMVDAINECYDGYFQELLVAIVLCVRDKPAYFAYRLYSAIHDFGFHNKTVIRILIARSEI
  DLLTIRKRYKERYGKSLFHDIRNFASGHYKKALLAICAGDAEDY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007193
• ORF Size: 972 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007193.5
• RefSeq Size: 1428 bp
• RefSeq ORF: 975 bp
• Locus ID: 11199
• UniProt ID: Q9UJ72
• Cytogenetics: 4q32.3
• Gene Summary: This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. The function of this gene has not yet been determined. [provided by RefSeq, Jul 2008]