DOCK8 (NM_203447) Human Tagged ORF Clone

CAT#: RC229462

DOCK8 (Myc-DDK-tagged)-Human dedicator of cytokinesis 8 (DOCK8), transcript variant 1

Product Images

Circular map for RC229462

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DOCK8
• Synonyms: HEL-205; MRD2; ZIR8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229462 representing NM_203447
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACTCTGCCGAGCGCAGAGCGCCGCGCGTTCGCGCTCAAGATCAACAGGTATTCTTCAGCGGAAA
  TAAGGAAACAGTTTACTCTCCCACCAAACCTTGGCCAGTACCATCGACAGAGCATAAGTACCTCTGGCTT
  CCCCTCTCTTCAACTACCTCAGTTTTATGACCCTGTGGAGCCAGTGGACTTTGAAGGACTTCTGATGACA
  CACCTGAACAGCCTGGATGTGCAGCTTGCCCAGGAGCTCGGGGACTTCACTGATGACGACTTGGACGTGG
  TGTTCACGCCAAAGGAATGTAGGACTTTGCAGCCCTCTTTGCCGGAGGAAGGGGTTGAACTGGACCCTCA
  TGTCAGGGACTGTGTTCAGACCTACATCCGTGAGTGGCTAATCGTGAACCGGAAAAACCAAGGAAGTCCA
  GAAATCTGTGGCTTTAAAAAGACTGGATCTCGAAAAGATTTTCACAAGACGCTTCCGAAACAGACGTTTG
  AGTCGGAAACCTTGGAGTGCAGTGAACCCGCTGCTCAGGCAGGCCCCCGCCACTTAAACGTGCTGTGCGA
  CGTGTCTGGGAAAGGCCCCGTCACTGCCTGTGACTTTGACCTCCGCAGCCTGCAGCCTGACAAGCGGCTA
  GAAAACCTCCTGCAGCAAGTGAGTGCCGAGGACTTTGAGAAGCAGAACGAGGAGGCCCGGAGGACCAATA
  GGCAGGCCGAGCTCTTTGCCCTTTACCCATCAGTGGACGAGGAGGATGCTGTGGAAATACGTCCAGTACC
  AGAATGTCCCAAGGAACACCTGGGCAACAGAATATTGGTCAAGTTGCTGACCTTGAAGTTCGAGATTGAA
  ATTGAGCCCCTGTTTGCCAGCATTGCCCTCTACGATGTTAAAGAAAGGAAAAAGATCTCAGAAAATTTTC
  ACTGTGACCTGAACTCTGACCAGTTCAAAGGATTTCTGCGAGCTCACACGCCTTCAGTGGCCGCATCAAG
  TCAGGCGAGATCTGCAGTCTTCTCAGTCACCTACCCGTCCTCAGACATCTACCTGGTAGTCAAGATTGAA
  AAAGTCCTGCAGCAGGGAGAGATTGGAGACTGTGCAGAGCCCTACACGGTTATCAAAGAAAGTGATGGTG
  GAAAGAGTAAAGAAAAGATTGAAAAACTAAAACTCCAAGCTGAATCCTTCTGCCAGCGTTTGGGGAAATA
  CCGGATGCCCTTTGCCTGGGCACCCATAAGCTTATCAAGCTTCTTCAATGTCTCCACCCTTGAGAGGGAG
  GTAACTGATGTGGACTCTGTGGTTGGGAGAAGCTCAGTGGGTGAACGGAGGACATTGGCCCAATCTAGAA
  GGCTTTCTGAAAGAGCCCTCTCCTTGGAGGAAAATGGGGTTGGATCCAACTTCAAAACCTCCACTCTGAG
  CGTTAGCAGCTTTTTCAAGCAGGAAGGAGATCGCCTTAGCGATGAAGACTTATTCAAGTTTTTAGCTGAC
  TACAAAAGATCATCATCCTTACAGAGACGAGTCAAGTCAATTCCAGGCTTGCTAAGACTGGAGATTTCTA
  CAGCTCCAGAGATCATCAATTGCTGTCTGACTCCTGAAATGCTGCCCGTGAAACCCTTTCCTGAAAACCG
  GACACGCCCGCACAAAGAGATTTTGGAATTTCCAACACGAGAAGTATATGTCCCTCACACTGTGTACAGA
  AACCTTCTCTATGTCTACCCACAGAGGCTGAACTTTGTAAACAAACTAGCATCAGCCCGGAACATTACAA
  TAAAGATCCAGTTTATGTGTGGAGAAGATGCTAGCAATGCGATGCCGGTCATCTTTGGAAAATCCAGCGG
  GCCTGAATTTCTGCAGGAAGTGTACACAGCTGTTACATACCATAATAAGTCTCCTGACTTTTATGAAGAA
  GTGAAAATTAAGCTCCCCGCTAAGCTCACAGTAAATCACCACCTCCTGTTCACCTTCTACCATATCAGCT
  GTCAGCAGAAGCAAGGAGCCTCCGTGGAAACTCTCCTGGGATATTCATGGCTGCCAATTCTCTTAAATGA
  ACGTCTTCAAACTGGATCCTACTGTCTCCCAGTTGCCTTGGAAAAATTGCCACCCAACTACTCCATGCAT
  TCTGCTGAGAAAGTCCCATTACAGAATCCTCCCATTAAGTGGGCTGAAGGACATAAGGGAGTATTTAATA
  TTGAAGTGCAAGCTGTTTCTTCTGTACACACCCAGGACAACCACCTGGAGAAGTTCTTCACCCTCTGCCA
  CTCCCTGGAGAGCCAGGTGACCTTCCCCATCCGCGTGCTGGATCAGAAAATCAGCGAGATGGCGCTGGAG
  CATGAGCTGAAGCTCAGCATCATCTGCCTGAACTCCTCCCGCCTGGAGCCGCTCGTGCTCTTCCTGCACC
  TGGTGCTGGACAAGCTCTTCCAGCTGTCCGTGCAGCCCATGGTCATCGCTGGCCAGACAGCCAACTTCTC
  CCAGTTTGCCTTCGAGTCCGTGGTGGCCATCGCCAACAGTCTGCACAACAGCAAGGACCTGAGCAAGGAC
  CAGCATGGGAGGAACTGCCTGCTGGCTTCCTACGTGCACTACGTCTTCCGCCTGCCAGAGGTGCAAAGGG
  ATGTGCCCAAGTCAGGCGCTCCCACTGCCCTCCTAGACCCTCGGAGCTACCACACGTATGGCCGCACATC
  AGCTGCTGCTGTGAGTTCAAAGCTGCTGCAGGCCCGGGTGATGAGCAGCAGTAACCCAGACCTCGCGGGG
  ACACACTCCGCAGCAGACGAGGAAGTGAAGAACATCATGTCTTCAAAGATCGCCGATCGCAACTGCAGCC
  GAATGTCTTACTATTGCTCTGGCAGTAGTGATGCTCCAAGTTCACCTGCAGCCCCAAGGCCAGCCAGCAA
  AAAGCATTTCCATGAGGAGCTTGCCCTTCAGATGGTGGTCAGCACCGGAATGGTGAGAGAAACAGTCTTC
  AAGTATGCCTGGTTCTTCTTTGAGCTTCTGGTGAAAAGCATGGCCCAGCACGTACATAACATGGACAAAC
  GGGACAGTTTTCGGAGGACTCGTTTTTCTGACCGTTTCATGGATGACATAACTACTATTGTTAATGTGGT
  CACCTCGGAAATTGCAGCCCTTTTAGTAAAACCACAGAAGGAAAATGAACAGGCGGAAAAGATGAACATC
  AGCCTGGCTTTCTTCTTGTATGACCTTCTCTCCCTCATGGATCGGGGCTTTGTGTTTAACCTCATCAGAC
  ATTATTGCAGCCAGCTGTCAGCCAAGCTCAGTAACCTTCCAACGCTCATTTCCATGAGGCTAGAGTTCCT
  GAGAATCCTCTGTAGCCATGAGCATTACCTCAATCTGAACCTTTTTTTTATGAATGCTGATACTGCTCCA
  ACATCTCCTTGTCCTTCCATATCTTCCCAGAACTCAAGCTCCTGCTCCAGCTTCCAGGACCAGAAGATCG
  CCAGCATGTTCGATCTGACTTCCGAGTACCGCCAGCAGCACTTCCTCACCGGGCTCCTCTTCACAGAACT
  GGCTGCTGCCCTGGATGCCGAAGGGGAAGGAATCAGCAAAGTACAAAGGAAAGCTGTCAGTGCAATTCAC
  AGCCTGCTAAGTTCTCACGACCTGGACCCACGCTGTGTCAAACCAGAGGTGAAGGTCAAAATCGCCGCCC
  TTTACCTACCTTTAGTTGGCATCATTTTGGATGCTTTGCCACAGCTCTGTGACTTTACAGTTGCAGATAC
  TCGCAGATACCGCACCAGTGGCTCGGATGAAGAACAAGAAGGAGCCGGTGCCATTAACCAGAATGTGGCT
  CTGGCCATAGCAGGGAATAATTTCAATTTGAAAACAAGTGGAATAGTGCTGTCTTCCTTGCCCTATAAGC
  AGTACAACATGCTGAACGCGGACACTACTCGCAACCTCATGATCTGCTTCCTCTGGATCATGAAAAATGC
  TGATCAGAGCCTCATTAGGAAGTGGATTGCTGACCTGCCATCAACGCAGCTCAACAGGATTTTAGATCTA
  CTTTTCATCTGTGTGTTATGTTTTGAGTATAAGGGAAAACAGAGTTCTGACAAAGTCAGTACCCAAGTCC
  TGCAGAAGTCAAGGGATGTCAAGGCCCGGCTGGAAGAGGCTTTGCTCCGTGGGGAAGGGGCCAGAGGGGA
  GATGATGCGCCGCCGGGCTCCAGGGAACGACCGATTTCCAGGCCTAAATGAAAATTTGAGATGGAAGAAA
  GAGCAGACACATTGGCGGCAAGCTAATGAGAAGCTAGATAAAACAAAGGCCGAGTTAGATCAAGAAGCCT
  TGATCAGTGGCAATCTGGCTACAGAAGCACATTTAATCATCCTGGATATGCAGGAAAACATTATCCAGGC
  GAGCTCGGCTCTGGACTGTAAAGACAGCCTGCTGGGAGGTGTTCTGAGGGTGCTGGTGAATTCTCTGAAC
  TGTGATCAGAGTACCACCTACCTGACTCACTGCTTTGCAACACTCCGTGCTCTCATCGCCAAGTTTGGAG
  ACTTACTCTTCGAAGAGGAGGTGGAACAGTGTTTCGACCTATGTCACCAAGTCCTGCACCACTGCAGCAG
  CAGCATGGATGTCACCCGGAGCCAAGCCTGTGCCACCCTTTACCTCCTCATGAGGTTCAGTTTTGGAGCC
  ACCAGTAATTTTGCAAGAGTAAAGATGCAAGTAACCATGTCCCTGGCATCTTTGGTGGGAAGAGCACCAG
  ACTTTAATGAAGAGCACCTGAGAAGATCCTTGAGGACAATTTTGGCCTATTCAGAAGAGGACACAGCCAT
  GCAGATGACTCCTTTTCCCACCCAGGTGGAGGAACTTCTCTGTAATCTGAATAGCATCTTATATGACACA
  GTGAAAATGAGGGAATTTCAGGAAGATCCTGAGATGCTTATGGATCTCATGTACAGAATTGCCAAGAGTT
  ACCAGGCATCTCCTGATCTGCGGCTGACCTGGCTCCAGAACATGGCAGAGAAACACACCAAGAAGAAGTG
  CTACACGGAGGCTGCCATGTGCCTGGTGCACGCCGCTGCGTTAGTGGCTGAGTATCTGAGCATGCTGGAG
  GACCACAGCTACCTGCCCGTGGGCAGTGTCAGCTTCCAGAATATTTCTTCCAATGTGCTGGAGGAGTCTG
  TGGTCTCTGAGGACACCCTGTCACCTGACGAGGATGGGGTGTGCGCAGGCCAGTACTTCACCGAGAGTGG
  CCTGGTAGGCCTCCTGGAGCAGGCCGCGGAGCTCTTCAGCACGGGAGGCTTATATGAGACAGTTAATGAG
  GTCTACAAGCTGGTCATCCCCATCCTAGAAGCGCATCGAGAATTCCGGAAGCTGACACTCACTCACAGCA
  AGCTGCAGAGAGCCTTCGACAGCATCGTTAACAAGGATCATAAGAGAATGTTTGGAACCTACTTCCGAGT
  TGGTTTCTTTGGATCCAAATTTGGGGATTTGGATGAACAGGAGTTTGTCTACAAAGAGCCTGCAATTACC
  AAGCTTCCTGAGATCTCACATAGACTAGAGGCATTTTATGGTCAATGTTTTGGTGCAGAATTTGTGGAAG
  TGATTAAAGACTCCACTCCTGTGGACAAAACCAAGTTGGATCCTAACAAGGCCTACATACAGATCACTTT
  TGTGGAGCCCTACTTTGATGAGTATGAGATGAAAGACAGGGTCACATACTTTGAGAAGAATTTCAACCTC
  CGGAGGTTCATGTACACCACCCCGTTCACCCTGGAGGGGCGGCCTCGGGGAGAGCTGCATGAGCAGTACA
  GAAGGAACACAGTCCTGACCACTATGCACGCCTTCCCCTACATCAAGACCAGGATCAGCGTCATCCAGAA
  GGAGGAGTTTGTTTTGACACCGATTGAAGTTGCCATTGAAGACATGAAGAAGAAGACCCTGCAGTTAGCA
  GTTGCCATTAACCAGGAGCCGCCTGATGCAAAGATGCTTCAGATGGTGCTGCAAGGCTCTGTGGGAGCTA
  CTGTAAATCAGGGACCACTGGAAGTAGCCCAAGTGTTTTTGGCTGAAATTCCTGCTGATCCAAAACTCTA
  TCGACATCACAACAAGTTGAGGTTATGCTTTAAGGAATTCATCATGAGATGTGGTGAAGCTGTAGAGAAA
  AACAAGCGTCTCATCACGGCAGACCAGAGGGAATATCAGCAGGAACTCAAAAAGAACTATAACAAGCTAA
  AAGAGAACCTCAGGCCAATGATCGAGCGGAAAATTCCAGAACTGTACAAGCCAATATTCAGAGTTGAGAG
  TCAAAAGAGGGACTCCTTCCACAGATCTAGTTTCAGGAAATGTGAAACCCAGTTGTCACAGGGCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229462 representing NM_203447
  Red=Cloning site Green=Tags(s)
  
  MATLPSAERRAFALKINRYSSAEIRKQFTLPPNLGQYHRQSISTSGFPSLQLPQFYDPVEPVDFEGLLMT
  HLNSLDVQLAQELGDFTDDDLDVVFTPKECRTLQPSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQGSP
  EICGFKKTGSRKDFHKTLPKQTFESETLECSEPAAQAGPRHLNVLCDVSGKGPVTACDFDLRSLQPDKRL
  ENLLQQVSAEDFEKQNEEARRTNRQAELFALYPSVDEEDAVEIRPVPECPKEHLGNRILVKLLTLKFEIE
  IEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSVAASSQARSAVFSVTYPSSDIYLVVKIE
  KVLQQGEIGDCAEPYTVIKESDGGKSKEKIEKLKLQAESFCQRLGKYRMPFAWAPISLSSFFNVSTLERE
  VTDVDSVVGRSSVGERRTLAQSRRLSERALSLEENGVGSNFKTSTLSVSSFFKQEGDRLSDEDLFKFLAD
  YKRSSSLQRRVKSIPGLLRLEISTAPEIINCCLTPEMLPVKPFPENRTRPHKEILEFPTREVYVPHTVYR
  NLLYVYPQRLNFVNKLASARNITIKIQFMCGEDASNAMPVIFGKSSGPEFLQEVYTAVTYHNKSPDFYEE
  VKIKLPAKLTVNHHLLFTFYHISCQQKQGASVETLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSMH
  SAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVTFPIRVLDQKISEMALE
  HELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLSKD
  QHGRNCLLASYVHYVFRLPEVQRDVPKSGAPTALLDPRSYHTYGRTSAAAVSSKLLQARVMSSSNPDLAG
  THSAADEEVKNIMSSKIADRNCSRMSYYCSGSSDAPSSPAAPRPASKKHFHEELALQMVVSTGMVRETVF
  KYAWFFFELLVKSMAQHVHNMDKRDSFRRTRFSDRFMDDITTIVNVVTSEIAALLVKPQKENEQAEKMNI
  SLAFFLYDLLSLMDRGFVFNLIRHYCSQLSAKLSNLPTLISMRLEFLRILCSHEHYLNLNLFFMNADTAP
  TSPCPSISSQNSSSCSSFQDQKIASMFDLTSEYRQQHFLTGLLFTELAAALDAEGEGISKVQRKAVSAIH
  SLLSSHDLDPRCVKPEVKVKIAALYLPLVGIILDALPQLCDFTVADTRRYRTSGSDEEQEGAGAINQNVA
  LAIAGNNFNLKTSGIVLSSLPYKQYNMLNADTTRNLMICFLWIMKNADQSLIRKWIADLPSTQLNRILDL
  LFICVLCFEYKGKQSSDKVSTQVLQKSRDVKARLEEALLRGEGARGEMMRRRAPGNDRFPGLNENLRWKK
  EQTHWRQANEKLDKTKAELDQEALISGNLATEAHLIILDMQENIIQASSALDCKDSLLGGVLRVLVNSLN
  CDQSTTYLTHCFATLRALIAKFGDLLFEEEVEQCFDLCHQVLHHCSSSMDVTRSQACATLYLLMRFSFGA
  TSNFARVKMQVTMSLASLVGRAPDFNEEHLRRSLRTILAYSEEDTAMQMTPFPTQVEELLCNLNSILYDT
  VKMREFQEDPEMLMDLMYRIAKSYQASPDLRLTWLQNMAEKHTKKKCYTEAAMCLVHAAALVAEYLSMLE
  DHSYLPVGSVSFQNISSNVLEESVVSEDTLSPDEDGVCAGQYFTESGLVGLLEQAAELFSTGGLYETVNE
  VYKLVIPILEAHREFRKLTLTHSKLQRAFDSIVNKDHKRMFGTYFRVGFFGSKFGDLDEQEFVYKEPAIT
  KLPEISHRLEAFYGQCFGAEFVEVIKDSTPVDKTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNFNL
  RRFMYTTPFTLEGRPRGELHEQYRRNTVLTTMHAFPYIKTRISVIQKEEFVLTPIEVAIEDMKKKTLQLA
  VAINQEPPDAKMLQMVLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVEK
  NKRLITADQREYQQELKKNYNKLKENLRPMIERKIPELYKPIFRVESQKRDSFHRSSFRKCETQLSQGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_203447
• ORF Size: 6297 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203447.3, NP_982272.2
• RefSeq ORF: 6300 bp
• Locus ID: 81704
• UniProt ID: Q8NF50
• Cytogenetics: 9p24.3
• MW: 238.3 kDa
• Gene Summary: This gene encodes a member of the DOCK180 family of guanine nucleotide exchange factors. Guanine nucleotide exchange factors interact with Rho GTPases and are components of intracellular signaling networks. Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jun 2010]