DOCK8 (NM_203447) Human Tagged ORF Clone

CAT#: RC222789

DOCK8 (Myc-DDK-tagged)-Human dedicator of cytokinesis 8 (DOCK8), transcript variant 1

Product Images

Circular map for RC222789

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DOCK8 (Cat# RC222789, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOCK8(Cat# TA803002). Positive lysates LY404295 (100ug) and LC404295 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY404295) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222789 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DOCK8 protein (Cat# TP322789). The protein was produced from HEK293T cells transfected with DOCK8 cDNA clone (Cat# RC222789) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DOCK8
• Synonyms: HEL-205; MRD2; ZIR8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222789 representing NM_203447
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACACACCTGAACAGCCTGGATGTGCAGCTTGCCCAGGAGCTCGGGGACTTCACTGATGACGACTTGG
  ACGTGGTGTTCACGCCAAAGGAATGTAGGACTTTGCAGCCCTCTTTGCCGGAGGAAGGGGTTGAACTGGA
  CCCTCATGTCAGGGACTGTGTTCAGACCTACATCCGTGAGTGGCTAATCGTGAACCGGAAAAACCAAGGA
  AGTCCAGAAATCTGTGGCTTTAAAAAGACTGGATCTCGAAAAGATTTTCACAAGACGCTTCCGAAACAGA
  CGTTTGAGTCGGAAACCTTGGAGTGCAGTGAACCCGCTGCTCAGGCAGGCCCCCGCCACTTAAACGTGCT
  GTGCGACGTGTCTGGGAAAGGCCCCGTCACTGCCTGTGACTTTGACCTCCGCAGCCTGCAGCCTGACAAG
  CGGCTAGAAAACCTCCTGCAGCAAGTGAGTGCCGAGGACTTTGAGAAGCAGAACGAGGAGGCCCGGAGGA
  CCAATAGGCAGGCCGAGCTCTTTGCCCTTTACCCATCAGTGGACGAGGAGGATGCTGTGGAAATACGTCC
  AGTACCAGAATGTCCCAAGGAACACCTGGGCAACAGAATATTGGTCAAGTTGCTGACCTTGAAGTTCGAG
  ATTGAAATTGAGCCCCTGTTTGCCAGCATTGCCCTCTACGATGTTAAAGAAAGGAAAAAGATCTCAGAAA
  ATTTTCACTGTGACCTGAACTCTGACCAGTTCAAAGGATTTCTGCGAGCTCACACGCCTTCAGTGGCCGC
  ATCAAGTCAGGCGAGATCTGCAGTCTTCTCAGTCACCTACCCGTCCTCAGACATCTACCTGGTAGTCAAG
  ATTGAAAAAGTCCTGCAGCAGGGAGAGATTGGAGACTGTGCAGAGCCCTACACGGTTATCAAAGAAAGTG
  ATGGTGGAAAGAGTAAAGAAAAGATTGAAAAACTAAAACTCCAAGCTGAATCCTTCTGCCAGCGTTTGGG
  GAAATACCGGATGCCCTTTGCCTGGGCACCCATAAGCTTATCAAGCTTCTTCAGTGTCTCCACCCTTGAG
  AGGGAGGTAACTGATGTGGACTCTGTGGTTGGGAGAAGCTCAGTGGGTGAACGGAGGACATTGGCCCAAT
  CTAGAAGGCTTTCTGAAAGAGCCCTCTCCTTGGAGGAAAATGGGGTTGGATCCAACTTCAAAACCTCCAC
  TCTGAGCGTTAGCATCTTTTTCAAGCAGGAAGGAGATCGCCTTAGCGATGAAGACTTATTCAAGTTTTTA
  GCTGACTACAAAAGATCATCATCCTTACAGAGACGAGTCAAGTCAATTCCAGGCTTGCTAAGACTGGAGA
  TTTCTACAGCTCCAGAGATCATCAATTGCTGTCTGACTCCTGAAATGCTGCCCGTGAAACCCTTTCCTGA
  AAACCGGACACGCCCGCACAAAGAGATTTTGGAATTTCCAACACGAGAAGTATATGTCCCTCACACTGTG
  TACAGAAACCTTCTCTATGTCTACCCACAGAGGCTGAACTTTGTAAACAAACTAGCATCAGCCCGGAACA
  TTACAATAAAGATCCAGTTTATGTGTGGAGAAGATGCTAGCAATGCGATGCCGGTCATCTTTGGAAAGTC
  CAGCGGGCCTGAATTTCTGCAGGAAGTGTACACAGCTGTTACATACCATAATAAGTCTCCTGACTTTTAT
  GAAGAAGTGAAAATTAAGCTCCCCGCTAAGCTCACAGTAAATCACCACCTCCTGTTCACCTTCTACCATA
  TCAGCTGTCAGCAGAAGCAAGGAGCCTCCGTGGAAACTCTCCTGGGATATTCATGGCTGCCAATTCTCTT
  AAATGAACGTCTTCAAACTGGATCCTACTGTCTCCCAGTTGCCTTGGAAAAATTGCCACCCAACTACTCC
  ATGCATTCTGCTGAGAAAGTCCCATTACAGAATCCTCCCATTAAGTGGGCTGAAGGACATAAGGGAGTAT
  TTAATATTGAAGTGCAAGCTGTTTCTTCTGTACACACCCAGGACAACCACCTGGAGAAGTTCTTCACCCT
  CTGCCACTCCCTGGAGAGCCAGGTGACCTTCCCCATCCGCGTGCTGGATCAGAAAATCAGCGAGATGGCG
  CTGGAGCATGAGCTGAAGCTCAGCATCATCTGCCTGAACTCCTCCCGCCTGGAGCCGCTCGTGCTCTTCC
  TGCACCTGGTGCTGGACAAGCTCTTCCAGCTGTCCGTGCAGCCCATGGTCATCGCTGGCCAGACAGCCAA
  CTTCTCCCAGTTTGCCTTCGAGTCCGTGGTGGCCATCGCCAACAGTCTGCACAACAGCAAGGACCTGAGC
  AAGGACCAGCATGGGAGGAACTGCCTGCTGGCTTCCTACGTGCACTACGTCTTCCGCCTGCCAGAGGTGC
  AAAGGGATGTGCCCAAGTCAGACGCTCCCACTGCCCTCCTAGACCCTCGGAGCTACCACACGTATGGCCG
  CACATCAGCTGCTGCTGTGAGTTCAAAGCTGCTGCAGGCCCGGGTGATGAGCAGCAGTAACCCAGACCTC
  GCGGGGACACACTCCGCAGCAGACGAGGAAGTGAAGAACATCATGTCTTCAAAGATCGCCGATCGCAACT
  GCAGCCGAATGTCTTACTATTGCTCTGGCAGTAGTGATGCTCCAAGTTCACCTGCAGCCCCAAGGCCAGC
  CAGCAAAAAGCATTTCCATGAGGAGCTTGCCCTTCAGATGGTGGTCAGCACCGGAATGGTGAGAGAAACA
  GTCTTCAAGTATGCCTGGTTCTTCTTTGAGCTTCTGGTGAAAAGCATGGCCCAGCACGTACATAACATGG
  ACAAACGGGACAGTTTTCGGAGGACTCGTTTTTCTGACCGTTTCATGGATGACATAACTACTATTGTTAA
  TGTGGTCACCTCGGAAATTGCAGCCCTTTTAGTAAAACCACAGAAGGAAAATGAACAGGCGGAAAAGATG
  AACATCAGCCTGGCTTTCTTCTTGTATGACCTTCTCTCCCTCATGGATCGGGGCTTTGTGTTTAACCTCA
  TCAGACATTATTGCAGCCAGCTGTCAGCCAAGCTCAGTAACCTTCCAACGCTCATTTCCATGAGGCTAGA
  GTTCCTGAGAATCCTCTGTAGCCATGAGCATTACCTCAATCTGAACCTTTTTTTTATGAATGCTGATACT
  GCTCCAACATCTCCTTGTCCTTCCATATCTTCCCAGAACTCAAGCTCCTGCTCCAGCTTCCAGGACCAGA
  AGATCGCCAGCATGTTCGATCTGACTTCCGAGTACCGCCAGCAGCACTTCCTCACCGGGCTCCTCTTCAC
  AGAACTGGCTGCTGCCCTGGATGCCGAAGGGGAAGGAATCAGCAAAGTACAAAGGAAAGCTGTCAGTGCA
  ATTCACAGCCTGCTAAGTTCTCACGACCTGGACCCACGCTGTGTCAAACCAGAGGTGAAGGTCAAAATCG
  CCGCCCTTTACCTACCTTTAGTTGGCATCATTTTGGATGCTTTGCCACAGCTCTGTGACTTTACAGTTGC
  AGATACTCGCAGATACCGCACCAGTGGCTCGGATGAAGAACAAGAAGGAGCCGGTGCCATTAACCAGAAT
  GTGGCTCTGGCCATAGCAGGGAATAATTTCAATTTGAAAACAAGTGGAATAGTGCTGTCTTCCTTGCCCT
  ATAAGCAGTACAACATGCTGAACGCGGACACTACTCGCAACCTCATGATCTGCTTCCTCTGGATCATGAA
  AAATGCTGATCAGAGCCTCATTAGGAAGTGGATTGCTGACCTGCCATCAACGCAGCTCAACAGGATTTTA
  GATCTACTTTTCATCTGTGTGTTATGTTTTGAGTATAAGGGAAAACAGAGTTCTGACAAAGTCAGTACCC
  AAGTCCTGCAGAAGTCAAGGGATGTCAAGGCCCGGCTGGAAGAGGCTTTGCTCCGTGGGGAAGGGGCCAG
  AGGGGAGATGATGCGCCGCCGGGCTCCAGGGAACGACCGATTTCCAGGCCTAAATGAAAATTTGAGATGG
  AAGAAAGAGCAGACACATTGGCGGCAAGCTAATGAGAAGCTAGATAAAACAAAGGCCGAGTTAGATCAAG
  AAGCCTTGATCAGTGGCAATCTGGCTACAGAAGCACATTTAATCATCCTGGATATGCAGGAAAACATTAT
  CCAGGCGAGCTCGGCTCTGGACTGTAAAGACAGCCTGCTGGGAGGTGTTCTGAGGGTGCTGGTGAATTCT
  CTGAACTGTGATCAGAGTACCACCTACCTGACTCACTGCTTTGCAACACTCCGTGCTCTCATCGCCAAGT
  TTGGAGACTTACTCTTCGAAGAGGAGGTGGAACAGTGTTTCGACCTATGTCACCAAGTCCTGCACCACTG
  CAGCAGCAGCATGGATGTCACCCGGAGCCAAGCCTGTGCCACCCTTTACCTCCTCATGAGGTTCAGTTTT
  GGAGCCACCAGTAATTTTGCAAGAGTAAAGATGCAAGTAACCATGTCCCTGGCATCTTTGGTGGGAAGAG
  CACCAGACTTTAATGAAGAGCACCTGAGAAGATCCTTGAGGACAATTTTGGCCTATTCAGAAGAGGACAC
  AGCCATGCAGATGACTCCTTTTCCCACCCAGGTGGAGGAACTTCTCTGTAATCTGAATAGCATCTTATAT
  GACACAGTGAAAATGAGGGAATTTCAGGAAGATCCTGAGATGCTTATGGATCTCATGTACAGAATTGCCA
  AGAGTTACCAGGCATCTCCTGATCTGCGGCTGACCTGGCTCCAGAACATGGCAGAGAAACACACCAAGAA
  GAAGTGCTACACGGAGGCTGCCATGTGCCTGGTGCACGCCGCTGCGTTAGTGGCTGAGTATCTGAGCATG
  CTGGAGGACCACAGCTACCTGCCCGTGGGCAGTGTCAGCTTCCAGAATATTTCTTCCAATGTGCTGGAGG
  AGTCTGTGGTCTCTGAGGACACCCTGTCACCTGACGAGGATGGGGTGTGCGCAGGCCAGTACTTCACCGA
  GAGTGGCCTGGTAGGCCTCCTGGAGCAGGCCGCGGAGCTCTTCAGCACGGGAGGCTTATATGAGACAGTT
  AATGAGGTCTACAAGCTGGTCATCCCCATCCTAGAAGCGCATCGAGAATTCCGGAAGCTGACACTCACTC
  ACAGCAAGCTGCAGAGAGCCTTCGACAGCATCGTTAACAAGGATCATAAGAGAATGTTTGGAACCTACTT
  CCGAGTTGGTTTCTTTGGATCCAAATTTGGGGATTTGGATGAACAGGAATTTGTCTACAAAGAGCCTGCA
  ATTACCAAGCTTCCTGAGATCTCACATAGACTAGAGGCATTTTATGGTCAATGTTTTGGTGCAGAATTTG
  TGGAAGTGATTAAAGACTCCACTCCTGTGGACAAAACCAAGTTGGATCCTAACAAGGCCTACATACAGAT
  CACTTTTGTGGAGCCCTACTTTGATGAGTATGAGATGAAAGACAGGGTCACATACTTTGAGAAGAATTTC
  AACCTCCGGAGGTTCATGTACACCACCCCGTTCACCCTGGAGGGGCGGCCTCGGGGAGAGCTGCATGAGC
  AGTACAGAAGGAACACAGTCCTGACCACTATGCACGCCTTCCCCTTCATCAAGACCAGGATCAGCGTCAT
  CCAGAAGGAGGAGTTTGTTTTGACACCGATTGAAGTTGCCATTGAAGACATGAAGAAGAAGACCCTGCAG
  TTAGCAGTTGCCATTAACCAGGAGCCGCCTGATGCAAAGATGCTTCAGATGGTGCTGCAAGGCTCTGTGG
  GAGCTACTGTAAATCAGGGACCACTGGAAGTAGCCCAAGTGTTTTTGGCTGAAATTCCTGCTGATCCAAA
  ACTCTATCGACATCACAACAAGTTGAGGTTATGCTTTAAGGAATTCATCATGAGATGTGGTGAAGCTGTA
  GAGAAAAACAAGCGTCTCATCACGGCAGACCAGAGGGAATATCAGCAGGAACTCAAAAAGAACTATAACA
  AGCTAAAAGAGAACCTCAGGCCAATGATCGAGCGGAAAATTCCAGAACTGTACAAGCCAATATTCAGAGT
  TGAGAGTCAAAAGAGGGACTCCTTCCACAGATCTAGTTTCAGGAAATGTGAAACCCAGTTGTCACAGGGC
  AGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222789 representing NM_203447
  Red=Cloning site Green=Tags(s)
  
  MTHLNSLDVQLAQELGDFTDDDLDVVFTPKECRTLQPSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQG
  SPEICGFKKTGSRKDFHKTLPKQTFESETLECSEPAAQAGPRHLNVLCDVSGKGPVTACDFDLRSLQPDK
  RLENLLQQVSAEDFEKQNEEARRTNRQAELFALYPSVDEEDAVEIRPVPECPKEHLGNRILVKLLTLKFE
  IEIEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSVAASSQARSAVFSVTYPSSDIYLVVK
  IEKVLQQGEIGDCAEPYTVIKESDGGKSKEKIEKLKLQAESFCQRLGKYRMPFAWAPISLSSFFSVSTLE
  REVTDVDSVVGRSSVGERRTLAQSRRLSERALSLEENGVGSNFKTSTLSVSIFFKQEGDRLSDEDLFKFL
  ADYKRSSSLQRRVKSIPGLLRLEISTAPEIINCCLTPEMLPVKPFPENRTRPHKEILEFPTREVYVPHTV
  YRNLLYVYPQRLNFVNKLASARNITIKIQFMCGEDASNAMPVIFGKSSGPEFLQEVYTAVTYHNKSPDFY
  EEVKIKLPAKLTVNHHLLFTFYHISCQQKQGASVETLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYS
  MHSAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVTFPIRVLDQKISEMA
  LEHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLS
  KDQHGRNCLLASYVHYVFRLPEVQRDVPKSDAPTALLDPRSYHTYGRTSAAAVSSKLLQARVMSSSNPDL
  AGTHSAADEEVKNIMSSKIADRNCSRMSYYCSGSSDAPSSPAAPRPASKKHFHEELALQMVVSTGMVRET
  VFKYAWFFFELLVKSMAQHVHNMDKRDSFRRTRFSDRFMDDITTIVNVVTSEIAALLVKPQKENEQAEKM
  NISLAFFLYDLLSLMDRGFVFNLIRHYCSQLSAKLSNLPTLISMRLEFLRILCSHEHYLNLNLFFMNADT
  APTSPCPSISSQNSSSCSSFQDQKIASMFDLTSEYRQQHFLTGLLFTELAAALDAEGEGISKVQRKAVSA
  IHSLLSSHDLDPRCVKPEVKVKIAALYLPLVGIILDALPQLCDFTVADTRRYRTSGSDEEQEGAGAINQN
  VALAIAGNNFNLKTSGIVLSSLPYKQYNMLNADTTRNLMICFLWIMKNADQSLIRKWIADLPSTQLNRIL
  DLLFICVLCFEYKGKQSSDKVSTQVLQKSRDVKARLEEALLRGEGARGEMMRRRAPGNDRFPGLNENLRW
  KKEQTHWRQANEKLDKTKAELDQEALISGNLATEAHLIILDMQENIIQASSALDCKDSLLGGVLRVLVNS
  LNCDQSTTYLTHCFATLRALIAKFGDLLFEEEVEQCFDLCHQVLHHCSSSMDVTRSQACATLYLLMRFSF
  GATSNFARVKMQVTMSLASLVGRAPDFNEEHLRRSLRTILAYSEEDTAMQMTPFPTQVEELLCNLNSILY
  DTVKMREFQEDPEMLMDLMYRIAKSYQASPDLRLTWLQNMAEKHTKKKCYTEAAMCLVHAAALVAEYLSM
  LEDHSYLPVGSVSFQNISSNVLEESVVSEDTLSPDEDGVCAGQYFTESGLVGLLEQAAELFSTGGLYETV
  NEVYKLVIPILEAHREFRKLTLTHSKLQRAFDSIVNKDHKRMFGTYFRVGFFGSKFGDLDEQEFVYKEPA
  ITKLPEISHRLEAFYGQCFGAEFVEVIKDSTPVDKTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNF
  NLRRFMYTTPFTLEGRPRGELHEQYRRNTVLTTMHAFPFIKTRISVIQKEEFVLTPIEVAIEDMKKKTLQ
  LAVAINQEPPDAKMLQMVLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAV
  EKNKRLITADQREYQQELKKNYNKLKENLRPMIERKIPELYKPIFRVESQKRDSFHRSSFRKCETQLSQG
  S
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_203447
• ORF Size: 6093 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203447.1, NP_982272.1
• RefSeq Size: 7257 bp
• RefSeq ORF: 6300 bp
• Locus ID: 81704
• UniProt ID: Q8NF50
• Cytogenetics: 9p24.3
• MW: 238.3 kDa
• Gene Summary: This gene encodes a member of the DOCK180 family of guanine nucleotide exchange factors. Guanine nucleotide exchange factors interact with Rho GTPases and are components of intracellular signaling networks. Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jun 2010]