DOCK8 (NM_203447) Human Tagged ORF Clone

CAT#: RC222789

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DOCK8 (Myc-DDK-tagged)-Human dedicator of cytokinesis 8 (DOCK8), transcript variant 1



  "NM_203447" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 2,265.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol DOCK8
Synonyms HEL-205; MRD2; ZIR8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC222789 representing NM_203447
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACACACCTGAACAGCCTGGATGTGCAGCTTGCCCAGGAGCTCGGGGACTTCACTGATGACGACTTGG
ACGTGGTGTTCACGCCAAAGGAATGTAGGACTTTGCAGCCCTCTTTGCCGGAGGAAGGGGTTGAACTGGA
CCCTCATGTCAGGGACTGTGTTCAGACCTACATCCGTGAGTGGCTAATCGTGAACCGGAAAAACCAAGGA
AGTCCAGAAATCTGTGGCTTTAAAAAGACTGGATCTCGAAAAGATTTTCACAAGACGCTTCCGAAACAGA
CGTTTGAGTCGGAAACCTTGGAGTGCAGTGAACCCGCTGCTCAGGCAGGCCCCCGCCACTTAAACGTGCT
GTGCGACGTGTCTGGGAAAGGCCCCGTCACTGCCTGTGACTTTGACCTCCGCAGCCTGCAGCCTGACAAG
CGGCTAGAAAACCTCCTGCAGCAAGTGAGTGCCGAGGACTTTGAGAAGCAGAACGAGGAGGCCCGGAGGA
CCAATAGGCAGGCCGAGCTCTTTGCCCTTTACCCATCAGTGGACGAGGAGGATGCTGTGGAAATACGTCC
AGTACCAGAATGTCCCAAGGAACACCTGGGCAACAGAATATTGGTCAAGTTGCTGACCTTGAAGTTCGAG
ATTGAAATTGAGCCCCTGTTTGCCAGCATTGCCCTCTACGATGTTAAAGAAAGGAAAAAGATCTCAGAAA
ATTTTCACTGTGACCTGAACTCTGACCAGTTCAAAGGATTTCTGCGAGCTCACACGCCTTCAGTGGCCGC
ATCAAGTCAGGCGAGATCTGCAGTCTTCTCAGTCACCTACCCGTCCTCAGACATCTACCTGGTAGTCAAG
ATTGAAAAAGTCCTGCAGCAGGGAGAGATTGGAGACTGTGCAGAGCCCTACACGGTTATCAAAGAAAGTG
ATGGTGGAAAGAGTAAAGAAAAGATTGAAAAACTAAAACTCCAAGCTGAATCCTTCTGCCAGCGTTTGGG
GAAATACCGGATGCCCTTTGCCTGGGCACCCATAAGCTTATCAAGCTTCTTCAGTGTCTCCACCCTTGAG
AGGGAGGTAACTGATGTGGACTCTGTGGTTGGGAGAAGCTCAGTGGGTGAACGGAGGACATTGGCCCAAT
CTAGAAGGCTTTCTGAAAGAGCCCTCTCCTTGGAGGAAAATGGGGTTGGATCCAACTTCAAAACCTCCAC
TCTGAGCGTTAGCATCTTTTTCAAGCAGGAAGGAGATCGCCTTAGCGATGAAGACTTATTCAAGTTTTTA
GCTGACTACAAAAGATCATCATCCTTACAGAGACGAGTCAAGTCAATTCCAGGCTTGCTAAGACTGGAGA
TTTCTACAGCTCCAGAGATCATCAATTGCTGTCTGACTCCTGAAATGCTGCCCGTGAAACCCTTTCCTGA
AAACCGGACACGCCCGCACAAAGAGATTTTGGAATTTCCAACACGAGAAGTATATGTCCCTCACACTGTG
TACAGAAACCTTCTCTATGTCTACCCACAGAGGCTGAACTTTGTAAACAAACTAGCATCAGCCCGGAACA
TTACAATAAAGATCCAGTTTATGTGTGGAGAAGATGCTAGCAATGCGATGCCGGTCATCTTTGGAAAGTC
CAGCGGGCCTGAATTTCTGCAGGAAGTGTACACAGCTGTTACATACCATAATAAGTCTCCTGACTTTTAT
GAAGAAGTGAAAATTAAGCTCCCCGCTAAGCTCACAGTAAATCACCACCTCCTGTTCACCTTCTACCATA
TCAGCTGTCAGCAGAAGCAAGGAGCCTCCGTGGAAACTCTCCTGGGATATTCATGGCTGCCAATTCTCTT
AAATGAACGTCTTCAAACTGGATCCTACTGTCTCCCAGTTGCCTTGGAAAAATTGCCACCCAACTACTCC
ATGCATTCTGCTGAGAAAGTCCCATTACAGAATCCTCCCATTAAGTGGGCTGAAGGACATAAGGGAGTAT
TTAATATTGAAGTGCAAGCTGTTTCTTCTGTACACACCCAGGACAACCACCTGGAGAAGTTCTTCACCCT
CTGCCACTCCCTGGAGAGCCAGGTGACCTTCCCCATCCGCGTGCTGGATCAGAAAATCAGCGAGATGGCG
CTGGAGCATGAGCTGAAGCTCAGCATCATCTGCCTGAACTCCTCCCGCCTGGAGCCGCTCGTGCTCTTCC
TGCACCTGGTGCTGGACAAGCTCTTCCAGCTGTCCGTGCAGCCCATGGTCATCGCTGGCCAGACAGCCAA
CTTCTCCCAGTTTGCCTTCGAGTCCGTGGTGGCCATCGCCAACAGTCTGCACAACAGCAAGGACCTGAGC
AAGGACCAGCATGGGAGGAACTGCCTGCTGGCTTCCTACGTGCACTACGTCTTCCGCCTGCCAGAGGTGC
AAAGGGATGTGCCCAAGTCAGACGCTCCCACTGCCCTCCTAGACCCTCGGAGCTACCACACGTATGGCCG
CACATCAGCTGCTGCTGTGAGTTCAAAGCTGCTGCAGGCCCGGGTGATGAGCAGCAGTAACCCAGACCTC
GCGGGGACACACTCCGCAGCAGACGAGGAAGTGAAGAACATCATGTCTTCAAAGATCGCCGATCGCAACT
GCAGCCGAATGTCTTACTATTGCTCTGGCAGTAGTGATGCTCCAAGTTCACCTGCAGCCCCAAGGCCAGC
CAGCAAAAAGCATTTCCATGAGGAGCTTGCCCTTCAGATGGTGGTCAGCACCGGAATGGTGAGAGAAACA
GTCTTCAAGTATGCCTGGTTCTTCTTTGAGCTTCTGGTGAAAAGCATGGCCCAGCACGTACATAACATGG
ACAAACGGGACAGTTTTCGGAGGACTCGTTTTTCTGACCGTTTCATGGATGACATAACTACTATTGTTAA
TGTGGTCACCTCGGAAATTGCAGCCCTTTTAGTAAAACCACAGAAGGAAAATGAACAGGCGGAAAAGATG
AACATCAGCCTGGCTTTCTTCTTGTATGACCTTCTCTCCCTCATGGATCGGGGCTTTGTGTTTAACCTCA
TCAGACATTATTGCAGCCAGCTGTCAGCCAAGCTCAGTAACCTTCCAACGCTCATTTCCATGAGGCTAGA
GTTCCTGAGAATCCTCTGTAGCCATGAGCATTACCTCAATCTGAACCTTTTTTTTATGAATGCTGATACT
GCTCCAACATCTCCTTGTCCTTCCATATCTTCCCAGAACTCAAGCTCCTGCTCCAGCTTCCAGGACCAGA
AGATCGCCAGCATGTTCGATCTGACTTCCGAGTACCGCCAGCAGCACTTCCTCACCGGGCTCCTCTTCAC
AGAACTGGCTGCTGCCCTGGATGCCGAAGGGGAAGGAATCAGCAAAGTACAAAGGAAAGCTGTCAGTGCA
ATTCACAGCCTGCTAAGTTCTCACGACCTGGACCCACGCTGTGTCAAACCAGAGGTGAAGGTCAAAATCG
CCGCCCTTTACCTACCTTTAGTTGGCATCATTTTGGATGCTTTGCCACAGCTCTGTGACTTTACAGTTGC
AGATACTCGCAGATACCGCACCAGTGGCTCGGATGAAGAACAAGAAGGAGCCGGTGCCATTAACCAGAAT
GTGGCTCTGGCCATAGCAGGGAATAATTTCAATTTGAAAACAAGTGGAATAGTGCTGTCTTCCTTGCCCT
ATAAGCAGTACAACATGCTGAACGCGGACACTACTCGCAACCTCATGATCTGCTTCCTCTGGATCATGAA
AAATGCTGATCAGAGCCTCATTAGGAAGTGGATTGCTGACCTGCCATCAACGCAGCTCAACAGGATTTTA
GATCTACTTTTCATCTGTGTGTTATGTTTTGAGTATAAGGGAAAACAGAGTTCTGACAAAGTCAGTACCC
AAGTCCTGCAGAAGTCAAGGGATGTCAAGGCCCGGCTGGAAGAGGCTTTGCTCCGTGGGGAAGGGGCCAG
AGGGGAGATGATGCGCCGCCGGGCTCCAGGGAACGACCGATTTCCAGGCCTAAATGAAAATTTGAGATGG
AAGAAAGAGCAGACACATTGGCGGCAAGCTAATGAGAAGCTAGATAAAACAAAGGCCGAGTTAGATCAAG
AAGCCTTGATCAGTGGCAATCTGGCTACAGAAGCACATTTAATCATCCTGGATATGCAGGAAAACATTAT
CCAGGCGAGCTCGGCTCTGGACTGTAAAGACAGCCTGCTGGGAGGTGTTCTGAGGGTGCTGGTGAATTCT
CTGAACTGTGATCAGAGTACCACCTACCTGACTCACTGCTTTGCAACACTCCGTGCTCTCATCGCCAAGT
TTGGAGACTTACTCTTCGAAGAGGAGGTGGAACAGTGTTTCGACCTATGTCACCAAGTCCTGCACCACTG
CAGCAGCAGCATGGATGTCACCCGGAGCCAAGCCTGTGCCACCCTTTACCTCCTCATGAGGTTCAGTTTT
GGAGCCACCAGTAATTTTGCAAGAGTAAAGATGCAAGTAACCATGTCCCTGGCATCTTTGGTGGGAAGAG
CACCAGACTTTAATGAAGAGCACCTGAGAAGATCCTTGAGGACAATTTTGGCCTATTCAGAAGAGGACAC
AGCCATGCAGATGACTCCTTTTCCCACCCAGGTGGAGGAACTTCTCTGTAATCTGAATAGCATCTTATAT
GACACAGTGAAAATGAGGGAATTTCAGGAAGATCCTGAGATGCTTATGGATCTCATGTACAGAATTGCCA
AGAGTTACCAGGCATCTCCTGATCTGCGGCTGACCTGGCTCCAGAACATGGCAGAGAAACACACCAAGAA
GAAGTGCTACACGGAGGCTGCCATGTGCCTGGTGCACGCCGCTGCGTTAGTGGCTGAGTATCTGAGCATG
CTGGAGGACCACAGCTACCTGCCCGTGGGCAGTGTCAGCTTCCAGAATATTTCTTCCAATGTGCTGGAGG
AGTCTGTGGTCTCTGAGGACACCCTGTCACCTGACGAGGATGGGGTGTGCGCAGGCCAGTACTTCACCGA
GAGTGGCCTGGTAGGCCTCCTGGAGCAGGCCGCGGAGCTCTTCAGCACGGGAGGCTTATATGAGACAGTT
AATGAGGTCTACAAGCTGGTCATCCCCATCCTAGAAGCGCATCGAGAATTCCGGAAGCTGACACTCACTC
ACAGCAAGCTGCAGAGAGCCTTCGACAGCATCGTTAACAAGGATCATAAGAGAATGTTTGGAACCTACTT
CCGAGTTGGTTTCTTTGGATCCAAATTTGGGGATTTGGATGAACAGGAATTTGTCTACAAAGAGCCTGCA
ATTACCAAGCTTCCTGAGATCTCACATAGACTAGAGGCATTTTATGGTCAATGTTTTGGTGCAGAATTTG
TGGAAGTGATTAAAGACTCCACTCCTGTGGACAAAACCAAGTTGGATCCTAACAAGGCCTACATACAGAT
CACTTTTGTGGAGCCCTACTTTGATGAGTATGAGATGAAAGACAGGGTCACATACTTTGAGAAGAATTTC
AACCTCCGGAGGTTCATGTACACCACCCCGTTCACCCTGGAGGGGCGGCCTCGGGGAGAGCTGCATGAGC
AGTACAGAAGGAACACAGTCCTGACCACTATGCACGCCTTCCCCTTCATCAAGACCAGGATCAGCGTCAT
CCAGAAGGAGGAGTTTGTTTTGACACCGATTGAAGTTGCCATTGAAGACATGAAGAAGAAGACCCTGCAG
TTAGCAGTTGCCATTAACCAGGAGCCGCCTGATGCAAAGATGCTTCAGATGGTGCTGCAAGGCTCTGTGG
GAGCTACTGTAAATCAGGGACCACTGGAAGTAGCCCAAGTGTTTTTGGCTGAAATTCCTGCTGATCCAAA
ACTCTATCGACATCACAACAAGTTGAGGTTATGCTTTAAGGAATTCATCATGAGATGTGGTGAAGCTGTA
GAGAAAAACAAGCGTCTCATCACGGCAGACCAGAGGGAATATCAGCAGGAACTCAAAAAGAACTATAACA
AGCTAAAAGAGAACCTCAGGCCAATGATCGAGCGGAAAATTCCAGAACTGTACAAGCCAATATTCAGAGT
TGAGAGTCAAAAGAGGGACTCCTTCCACAGATCTAGTTTCAGGAAATGTGAAACCCAGTTGTCACAGGGC
AGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC222789 representing NM_203447
Red=Cloning site Green=Tags(s)

MTHLNSLDVQLAQELGDFTDDDLDVVFTPKECRTLQPSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQG
SPEICGFKKTGSRKDFHKTLPKQTFESETLECSEPAAQAGPRHLNVLCDVSGKGPVTACDFDLRSLQPDK
RLENLLQQVSAEDFEKQNEEARRTNRQAELFALYPSVDEEDAVEIRPVPECPKEHLGNRILVKLLTLKFE
IEIEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSVAASSQARSAVFSVTYPSSDIYLVVK
IEKVLQQGEIGDCAEPYTVIKESDGGKSKEKIEKLKLQAESFCQRLGKYRMPFAWAPISLSSFFSVSTLE
REVTDVDSVVGRSSVGERRTLAQSRRLSERALSLEENGVGSNFKTSTLSVSIFFKQEGDRLSDEDLFKFL
ADYKRSSSLQRRVKSIPGLLRLEISTAPEIINCCLTPEMLPVKPFPENRTRPHKEILEFPTREVYVPHTV
YRNLLYVYPQRLNFVNKLASARNITIKIQFMCGEDASNAMPVIFGKSSGPEFLQEVYTAVTYHNKSPDFY
EEVKIKLPAKLTVNHHLLFTFYHISCQQKQGASVETLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYS
MHSAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVTFPIRVLDQKISEMA
LEHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLS
KDQHGRNCLLASYVHYVFRLPEVQRDVPKSDAPTALLDPRSYHTYGRTSAAAVSSKLLQARVMSSSNPDL
AGTHSAADEEVKNIMSSKIADRNCSRMSYYCSGSSDAPSSPAAPRPASKKHFHEELALQMVVSTGMVRET
VFKYAWFFFELLVKSMAQHVHNMDKRDSFRRTRFSDRFMDDITTIVNVVTSEIAALLVKPQKENEQAEKM
NISLAFFLYDLLSLMDRGFVFNLIRHYCSQLSAKLSNLPTLISMRLEFLRILCSHEHYLNLNLFFMNADT
APTSPCPSISSQNSSSCSSFQDQKIASMFDLTSEYRQQHFLTGLLFTELAAALDAEGEGISKVQRKAVSA
IHSLLSSHDLDPRCVKPEVKVKIAALYLPLVGIILDALPQLCDFTVADTRRYRTSGSDEEQEGAGAINQN
VALAIAGNNFNLKTSGIVLSSLPYKQYNMLNADTTRNLMICFLWIMKNADQSLIRKWIADLPSTQLNRIL
DLLFICVLCFEYKGKQSSDKVSTQVLQKSRDVKARLEEALLRGEGARGEMMRRRAPGNDRFPGLNENLRW
KKEQTHWRQANEKLDKTKAELDQEALISGNLATEAHLIILDMQENIIQASSALDCKDSLLGGVLRVLVNS
LNCDQSTTYLTHCFATLRALIAKFGDLLFEEEVEQCFDLCHQVLHHCSSSMDVTRSQACATLYLLMRFSF
GATSNFARVKMQVTMSLASLVGRAPDFNEEHLRRSLRTILAYSEEDTAMQMTPFPTQVEELLCNLNSILY
DTVKMREFQEDPEMLMDLMYRIAKSYQASPDLRLTWLQNMAEKHTKKKCYTEAAMCLVHAAALVAEYLSM
LEDHSYLPVGSVSFQNISSNVLEESVVSEDTLSPDEDGVCAGQYFTESGLVGLLEQAAELFSTGGLYETV
NEVYKLVIPILEAHREFRKLTLTHSKLQRAFDSIVNKDHKRMFGTYFRVGFFGSKFGDLDEQEFVYKEPA
ITKLPEISHRLEAFYGQCFGAEFVEVIKDSTPVDKTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNF
NLRRFMYTTPFTLEGRPRGELHEQYRRNTVLTTMHAFPFIKTRISVIQKEEFVLTPIEVAIEDMKKKTLQ
LAVAINQEPPDAKMLQMVLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAV
EKNKRLITADQREYQQELKKNYNKLKENLRPMIERKIPELYKPIFRVESQKRDSFHRSSFRKCETQLSQG
S

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_203447
ORF Size 6093 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_203447.1, NP_982272.1
RefSeq Size 7257 bp
RefSeq ORF 6300 bp
Locus ID 81704
UniProt ID Q8NF50
Cytogenetics 9p24.3
MW 238.3 kDa
Gene Summary This gene encodes a member of the DOCK180 family of guanine nucleotide exchange factors. Guanine nucleotide exchange factors interact with Rho GTPases and are components of intracellular signaling networks. Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jun 2010]

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