NMDAR2B (GRIN2B) (NM_000834) Human Tagged ORF Clone

CAT#: RC223623

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GRIN2B (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B)



  "NM_000834" in other vectors (6)

Reconstitution Protocol

USD 1,904.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol GRIN2B
Synonyms DEE27; EIEE27; GluN2B; hNR3; MRD6; NMDAR2B; NR2B; NR3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC223623 representing NM_000834
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCCCAGAGCGGAGTGCTGTTCTCCCAAGTTCTGGTTGGTGTTGGCCGTCCTGGCCGTGTCAGGCA
GCAGAGCTCGTTCTCAGAAGAGCCCCCCCAGCATTGGCATTGCTGTCATCCTCGTGGGCACTTCCGACGA
GGTGGCCATCAAGGATGCCCACGAGAAAGATGATTTCCACCATCTCTCCGTGGTACCCCGGGTGGAACTG
GTAGCCATGAATGAGACCGACCCAAAGAGCATCATCACCCGCATCTGTGATCTCATGTCTGACCGGAAGA
TCCAGGGGGTGGTGTTTGCTGATGACACAGACCAGGAAGCCATCGCCCAGATCCTCGATTTCATTTCAGC
ACAGACTCTCACCCCGATCCTGGGCATCCACGGGGGCTCCTCTATGATAATGGCAGATAAGGATGAATCC
TCCATGTTCTTCCAGTTTGGCCCATCAATTGAACAGCAAGCTTCCGTAATGCTCAACATCATGGAAGAAT
ATGACTGGTACATCTTTTCTATCGTCACCACCTATTTCCCTGGCTACCAGGACTTTGTAAACAAGATCCG
CAGCACCATTGAGAATAGCTTTGTGGGCTGGGAGCTAGAGGAGGTCCTCCTACTGGACATGTCCCTGGAC
GATGGAGATTCTAAGATCCAGAATCAGCTCAAGAAACTTCAAAGCCCCATCATTCTTCTTTACTGTACCA
AGGAAGAAGCCACCTACATCTTTGAAGTGGCCAACTCAGTAGGGCTGACTGGCTATGGCTACACGTGGAT
CGTGCCCAGTCTGGTGGCAGGGGATACAGACACAGTGCCTGCGGAGTTCCCCACTGGGCTCATCTCTGTA
TCATATGATGAATGGGACTATGGCCTCCCCGCCAGAGTGAGAGATGGAATTGCCATAATCACCACTGCTG
CTTCTGACATGCTGTCTGAGCACAGCTTCATCCCTGAGCCCAAAAGCAGTTGTTACAACACCCACGAGAA
GAGAATCTACCAGTCCAATATGCTAAATAGGTATCTGATCAATGTCACTTTTGAGGGGAGGAATTTGTCC
TTCAGTGAAGATGGCTACCAGATGCACCCGAAACTGGTGATAATTCTTCTGAACAAGGAGAGGAAGTGGG
AAAGGGTGGGGAAGTGGAAAGACAAGTCCCTGCAGATGAAGTACTATGTGTGGCCCCGAATGTGTCCAGA
GACTGAAGAGCAGGAGGATGACCATCTGAGCATTGTGACCCTGGAGGAGGCACCATTTGTCATTGTGGAA
AGTGTGGACCCTCTGAGTGGAACCTGCATGAGGAACACAGTCCCCTGCCAAAAACGCATAGTCACTGAGA
ATAAAACAGACGAGGAGCCGGGTTACATCAAAAAATGCTGCAAGGGGTTCTGTATTGACATCCTTAAGAA
AATTTCTAAATCTGTGAAGTTCACCTATGACCTTTACCTGGTTACCAATGGCAAGCATGGGAAGAAAATC
AATGGAACCTGGAATGGTATGATTGGAGAGGTGGTCATGAAGAGGGCCTACATGGCAGTGGGCTCACTCA
CCATCAATGAGGAACGATCGGAGGTGGTCGACTTCTCTGTGCCCTTCATAGAGACAGGCATCAGTGTCAT
GGTGTCACGCAGCAATGGGACTGTCTCACCTTCTGCCTTCTTAGAGCCATTCAGCGCTGACGTATGGGTG
ATGATGTTTGTGATGCTGCTCATCGTCTCAGCCGTGGCTGTCTTTGTCTTTGAGTACTTCAGCCCTGTGG
GTTATAACAGGTGCCTCGCTGATGGCAGAGAGCCTGGTGGACCCTCTTTCACCATCGGCAAAGCTATTTG
GTTGCTCTGGGGTCTGGTGTTTAACAACTCCGTACCTGTGCAGAACCCAAAGGGGACCACCTCCAAGATC
ATGGTGTCAGTGTGGGCCTTCTTTGCTGTCATCTTCCTGGCCAGCTACACTGCCAACTTAGCTGCCTTCA
TGATCCAAGAGGAATATGTGGACCAGGTTTCTGGCCTGAGCGACAAAAAGTTCCAGAGACCTAATGACTT
CTCACCCCCTTTCCGCTTTGGGACCGTGCCCAACGGCAGCACAGAGAGAAATATTCGCAATAACTATGCA
GAAATGCATGCCTACATGGGAAAGTTCAACCAGAGGGGTGTAGATGATGCATTGCTCTCCCTGAAAACAG
GGAAACTGGATGCCTTCATCTATGATGCAGCAGTGCTGAACTATATGGCAGGCAGAGATGAAGGCTGCAA
GCTGGTGACCATTGGCAGTGGGAAGGTCTTTGCTTCCACTGGCTATGGCATTGCCATCCAAAAAGATTCT
GGGTGGAAGCGCCAGGTGGACCTTGCTATCCTGCAGCTCTTTGGAGATGGGGAGATGGAAGAACTGGAAG
CTCTCTGGCTCACTGGCATTTGTCACAATGAGAAGAATGAGGTCATGAGCAGCCAGCTGGACATTGACAA
CATGGCAGGGGTCTTCTACATGTTGGGGGCGGCCATGGCTCTCAGCCTCATCACCTTCATCTGCGAACAC
CTTTTCTATTGGCAGTTCCGACATTGCTTTATGGGTGTCTGTTCTGGCAAGCCTGGCATGGTCTTCTCCA
TCAGCAGAGGTATCTACAGCTGCATCCATGGGGTGGCGATCGAGGAGCGCCAGTCTGTAATGAACTCCCC
CACTGCAACCATGAACAACACACACTCCAACATCCTGCGCCTGCTGCGCACGGCCAAGAACATGGCTAAC
CTGTCTGGTGTGAATGGCTCACCGCAGAGCGCCCTGGACTTCATCCGACGGGAGTCATCCGTCTATGACA
TCTCAGAGCACCGCCGCAGCTTCACGCATTCTGACTGCAAATCCTACAACAACCCGCCCTGTGAGGAGAA
CCTCTTCAGTGACTACATCAGTGAGGTAGAGAGAACGTTCGGGAACCTGCAGCTGAAGGACAGCAACGTG
TACCAAGATCACTACCACCATCACCACCGGCCCCATAGTATTGGCAGTGCCAGCTCCATCGATGGGCTCT
ACGACTGTGACAACCCACCCTTCACCACCCAGTCCAGGTCCATCAGCAAGAAGCCCCTGGACATCGGCCT
CCCCTCCTCCAAGCACAGCCAGCTCAGTGACCTGTACGGCAAATTCTCCTTCAAGAGCGACCGCTACAGT
GGCCACGACGACTTGATCCGCTCCGATGTCTCTGACATCTCAACCCACACCGTCACCTATGGGAACATCG
AGGGCAATGCCGCCAAGAGGCGTAAGCAGCAATATAAGGACAGCCTGAAGAAGCGGCCTGCCTCGGCCAA
GTCCCGCAGGGAGTTTGACGAGATCGAGCTGGCCTACCGTCGCCGACCGCCCCGCTCCCCTGACCACAAG
CGCTACTTCAGGGACAAGGAAGGGCTACGGGACTTCTACCTGGACCAGTTCCGAACAAAGGAGAACTCAC
CCCACTGGGAGCACGTAGACCTGACCGACATCTACAAGGAGCGGAGTGATGACTTTAAGCGCGACTCCGT
CAGCGGAGGAGGGCCCTGTACCAACAGGTCTCACATCAAGCACGGGACGGGCGACAAACACGGCGTGGTC
AGCGGGGTACCTGCACCTTGGGAGAAGAACCTGACCAACGTGGAGTGGGAGGACCGGTCCGGGGGCAACT
TCTGCCGCAGCTGTCCCTCCAAGCTGCACAACTACTCCACGACGGTGACGGGTCAGAACTCGGGCAGGCA
GGCGTGCATCCGGTGTGAGGCTTGCAAGAAAGCAGGCAACCTGTATGACATCAGTGAGGACAACTCCCTG
CAGGAACTGGACCAGCCGGCTGCCCCAGTGGCGGTGACGTCAAACGCCTCCACCACTAAGTACCCTCAGA
GCCCGACTAATTCCAAGGCCCAGAAGAAGAACCGGAACAAACTGCGCCGGCAGCACTCCTACGACACCTT
CGTGGACCTGCAGAAGGAAGAAGCCGCCCTGGCCCCGCGCAGCGTAAGCCTGAAAGACAAGGGCCGATTC
ATGGATGGGAGCCCCTACGCCCACATGTTTGAGATGTCAGCTGGCGAGAGCACCTTTGCCAACAACAAGT
CCTCAGTGCCCACTGCCGGACATCACCACCACAACAACCCCGGCGGCGGGTACATGCTCAGCAAGTCGCT
CTACCCTGACCGGGTCACGCAAAACCCTTTCATCCCCACTTTTGGGGACGACCAGTGCTTGCTCCATGGC
AGCAAATCCTACTTCTTCAGGCAGCCCACGGTGGCGGGGGCGTCGAAAGCCAGGCCGGACTTCCGGGCCC
TTGTCACCAACAAGCCGGTGGTCTCGGCCCTTCATGGGGCCGTGCCAGCCCGTTTCCAGAAGGACATCTG
TATAGGGAACCAGTCCAACCCCTGTGTGCCTAACAACAAAAACCCCAGGGCTTTCAATGGCTCCAGCAAT
GGGCATGTTTATGAGAAACTTTCTAGTATTGAGTCTGATGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC223623 representing NM_000834
Red=Cloning site Green=Tags(s)

MKPRAECCSPKFWLVLAVLAVSGSRARSQKSPPSIGIAVILVGTSDEVAIKDAHEKDDFHHLSVVPRVEL
VAMNETDPKSIITRICDLMSDRKIQGVVFADDTDQEAIAQILDFISAQTLTPILGIHGGSSMIMADKDES
SMFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYFPGYQDFVNKIRSTIENSFVGWELEEVLLLDMSLD
DGDSKIQNQLKKLQSPIILLYCTKEEATYIFEVANSVGLTGYGYTWIVPSLVAGDTDTVPAEFPTGLISV
SYDEWDYGLPARVRDGIAIITTAASDMLSEHSFIPEPKSSCYNTHEKRIYQSNMLNRYLINVTFEGRNLS
FSEDGYQMHPKLVIILLNKERKWERVGKWKDKSLQMKYYVWPRMCPETEEQEDDHLSIVTLEEAPFVIVE
SVDPLSGTCMRNTVPCQKRIVTENKTDEEPGYIKKCCKGFCIDILKKISKSVKFTYDLYLVTNGKHGKKI
NGTWNGMIGEVVMKRAYMAVGSLTINEERSEVVDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWV
MMFVMLLIVSAVAVFVFEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKI
MVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVSGLSDKKFQRPNDFSPPFRFGTVPNGSTERNIRNNYA
EMHAYMGKFNQRGVDDALLSLKTGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGKVFASTGYGIAIQKDS
GWKRQVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQLDIDNMAGVFYMLGAAMALSLITFICEH
LFYWQFRHCFMGVCSGKPGMVFSISRGIYSCIHGVAIEERQSVMNSPTATMNNTHSNILRLLRTAKNMAN
LSGVNGSPQSALDFIRRESSVYDISEHRRSFTHSDCKSYNNPPCEENLFSDYISEVERTFGNLQLKDSNV
YQDHYHHHHRPHSIGSASSIDGLYDCDNPPFTTQSRSISKKPLDIGLPSSKHSQLSDLYGKFSFKSDRYS
GHDDLIRSDVSDISTHTVTYGNIEGNAAKRRKQQYKDSLKKRPASAKSRREFDEIELAYRRRPPRSPDHK
RYFRDKEGLRDFYLDQFRTKENSPHWEHVDLTDIYKERSDDFKRDSVSGGGPCTNRSHIKHGTGDKHGVV
SGVPAPWEKNLTNVEWEDRSGGNFCRSCPSKLHNYSTTVTGQNSGRQACIRCEACKKAGNLYDISEDNSL
QELDQPAAPVAVTSNASTTKYPQSPTNSKAQKKNRNKLRRQHSYDTFVDLQKEEAALAPRSVSLKDKGRF
MDGSPYAHMFEMSAGESTFANNKSSVPTAGHHHHNNPGGGYMLSKSLYPDRVTQNPFIPTFGDDQCLLHG
SKSYFFRQPTVAGASKARPDFRALVTNKPVVSALHGAVPARFQKDICIGNQSNPCVPNNKNPRAFNGSSN
GHVYEKLSSIESDV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000834
ORF Size 4452 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000834.5
RefSeq Size 6240 bp
RefSeq ORF 4455 bp
Locus ID 2904
UniProt ID Q13224
Cytogenetics 12p13.1
Domains lig_chan
Protein Families Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
Protein Pathways Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Huntington's disease, Long-term potentiation, Neuroactive ligand-receptor interaction, Systemic lupus erythematosus
MW 166.37 kDa
Gene Summary This gene encodes a member of the N-methyl-D-aspartate (NMDA) receptor family within the ionotropic glutamate receptor superfamily. The encoded protein is a subunit of the NMDA receptor ion channel which acts as an agonist binding site for glutamate. The NMDA receptors mediate a slow calcium-permeable component of excitatory synaptic transmission in the central nervous system. The NMDA receptors are heterotetramers of seven genetically encoded, differentially expressed subunits including NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The early expression of this gene in development suggests a role in brain development, circuit formation, synaptic plasticity, and cellular migration and differentiation. Naturally occurring mutations within this gene are associated with neurodevelopmental disorders including autism spectrum disorder, attention deficit hyperactivity disorder, epilepsy, and schizophrenia. [provided by RefSeq, Aug 2017]

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