NMDAR2B (GRIN2B) (NM_000834) Human Tagged ORF Clone

CAT#: RG223623

GRIN2B (tGFP-tagged) - Human glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B)

Product Images

Circular map for RG223623

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NMDAR2B
• Synonyms: DEE27; EIEE27; GluN2B; hNR3; MRD6; NMDAR2B; NR2B; NR3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223623 representing NM_000834
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCCCAGAGCGGAGTGCTGTTCTCCCAAGTTCTGGTTGGTGTTGGCCGTCCTGGCCGTGTCAGGCA
  GCAGAGCTCGTTCTCAGAAGAGCCCCCCCAGCATTGGCATTGCTGTCATCCTCGTGGGCACTTCCGACGA
  GGTGGCCATCAAGGATGCCCACGAGAAAGATGATTTCCACCATCTCTCCGTGGTACCCCGGGTGGAACTG
  GTAGCCATGAATGAGACCGACCCAAAGAGCATCATCACCCGCATCTGTGATCTCATGTCTGACCGGAAGA
  TCCAGGGGGTGGTGTTTGCTGATGACACAGACCAGGAAGCCATCGCCCAGATCCTCGATTTCATTTCAGC
  ACAGACTCTCACCCCGATCCTGGGCATCCACGGGGGCTCCTCTATGATAATGGCAGATAAGGATGAATCC
  TCCATGTTCTTCCAGTTTGGCCCATCAATTGAACAGCAAGCTTCCGTAATGCTCAACATCATGGAAGAAT
  ATGACTGGTACATCTTTTCTATCGTCACCACCTATTTCCCTGGCTACCAGGACTTTGTAAACAAGATCCG
  CAGCACCATTGAGAATAGCTTTGTGGGCTGGGAGCTAGAGGAGGTCCTCCTACTGGACATGTCCCTGGAC
  GATGGAGATTCTAAGATCCAGAATCAGCTCAAGAAACTTCAAAGCCCCATCATTCTTCTTTACTGTACCA
  AGGAAGAAGCCACCTACATCTTTGAAGTGGCCAACTCAGTAGGGCTGACTGGCTATGGCTACACGTGGAT
  CGTGCCCAGTCTGGTGGCAGGGGATACAGACACAGTGCCTGCGGAGTTCCCCACTGGGCTCATCTCTGTA
  TCATATGATGAATGGGACTATGGCCTCCCCGCCAGAGTGAGAGATGGAATTGCCATAATCACCACTGCTG
  CTTCTGACATGCTGTCTGAGCACAGCTTCATCCCTGAGCCCAAAAGCAGTTGTTACAACACCCACGAGAA
  GAGAATCTACCAGTCCAATATGCTAAATAGGTATCTGATCAATGTCACTTTTGAGGGGAGGAATTTGTCC
  TTCAGTGAAGATGGCTACCAGATGCACCCGAAACTGGTGATAATTCTTCTGAACAAGGAGAGGAAGTGGG
  AAAGGGTGGGGAAGTGGAAAGACAAGTCCCTGCAGATGAAGTACTATGTGTGGCCCCGAATGTGTCCAGA
  GACTGAAGAGCAGGAGGATGACCATCTGAGCATTGTGACCCTGGAGGAGGCACCATTTGTCATTGTGGAA
  AGTGTGGACCCTCTGAGTGGAACCTGCATGAGGAACACAGTCCCCTGCCAAAAACGCATAGTCACTGAGA
  ATAAAACAGACGAGGAGCCGGGTTACATCAAAAAATGCTGCAAGGGGTTCTGTATTGACATCCTTAAGAA
  AATTTCTAAATCTGTGAAGTTCACCTATGACCTTTACCTGGTTACCAATGGCAAGCATGGGAAGAAAATC
  AATGGAACCTGGAATGGTATGATTGGAGAGGTGGTCATGAAGAGGGCCTACATGGCAGTGGGCTCACTCA
  CCATCAATGAGGAACGATCGGAGGTGGTCGACTTCTCTGTGCCCTTCATAGAGACAGGCATCAGTGTCAT
  GGTGTCACGCAGCAATGGGACTGTCTCACCTTCTGCCTTCTTAGAGCCATTCAGCGCTGACGTATGGGTG
  ATGATGTTTGTGATGCTGCTCATCGTCTCAGCCGTGGCTGTCTTTGTCTTTGAGTACTTCAGCCCTGTGG
  GTTATAACAGGTGCCTCGCTGATGGCAGAGAGCCTGGTGGACCCTCTTTCACCATCGGCAAAGCTATTTG
  GTTGCTCTGGGGTCTGGTGTTTAACAACTCCGTACCTGTGCAGAACCCAAAGGGGACCACCTCCAAGATC
  ATGGTGTCAGTGTGGGCCTTCTTTGCTGTCATCTTCCTGGCCAGCTACACTGCCAACTTAGCTGCCTTCA
  TGATCCAAGAGGAATATGTGGACCAGGTTTCTGGCCTGAGCGACAAAAAGTTCCAGAGACCTAATGACTT
  CTCACCCCCTTTCCGCTTTGGGACCGTGCCCAACGGCAGCACAGAGAGAAATATTCGCAATAACTATGCA
  GAAATGCATGCCTACATGGGAAAGTTCAACCAGAGGGGTGTAGATGATGCATTGCTCTCCCTGAAAACAG
  GGAAACTGGATGCCTTCATCTATGATGCAGCAGTGCTGAACTATATGGCAGGCAGAGATGAAGGCTGCAA
  GCTGGTGACCATTGGCAGTGGGAAGGTCTTTGCTTCCACTGGCTATGGCATTGCCATCCAAAAAGATTCT
  GGGTGGAAGCGCCAGGTGGACCTTGCTATCCTGCAGCTCTTTGGAGATGGGGAGATGGAAGAACTGGAAG
  CTCTCTGGCTCACTGGCATTTGTCACAATGAGAAGAATGAGGTCATGAGCAGCCAGCTGGACATTGACAA
  CATGGCAGGGGTCTTCTACATGTTGGGGGCGGCCATGGCTCTCAGCCTCATCACCTTCATCTGCGAACAC
  CTTTTCTATTGGCAGTTCCGACATTGCTTTATGGGTGTCTGTTCTGGCAAGCCTGGCATGGTCTTCTCCA
  TCAGCAGAGGTATCTACAGCTGCATCCATGGGGTGGCGATCGAGGAGCGCCAGTCTGTAATGAACTCCCC
  CACCGCAACCATGAACAACACACACTCCAACATCCTGCGCCTGCTGCGCACGGCCAAGAACATGGCTAAC
  CTGTCTGGTGTGAATGGCTCACCGCAGAGCGCCCTGGACTTCATCCGACGGGAGTCATCCGTCTATGACA
  TCTCAGAGCACCGCCGCAGCTTCACGCATTCTGACTGCAAATCCTACAACAACCCGCCCTGTGAGGAGAA
  CCTCTTCAGTGACTACATCAGTGAGGTAGAGAGAACGTTCGGGAACCTGCAGCTGAAGGACAGCAACGTG
  TACCAAGATCACTACCACCATCACCACCGGCCCCATAGTATTGGCAGTGCCAGCTCCATCGATGGGCTCT
  ACGACTGTGACAACCCACCCTTCACCACCCAGTCCAGGTCCATCAGCAAGAAGCCCCTGGACATCGGCCT
  CCCCTCCTCCAAGCACAGCCAGCTCAGTGACCTGTACGGCAAATTCTCCTTCAAGAGCGACCGCTACAGT
  GGCCACGACGACTTGATCCGCTCCGATGTCTCTGACATCTCAACCCACACCGTCACCTATGGGAACATCG
  AGGGCAATGCCGCCAAGAGGCGTAAGCAGCAATATAAGGACAGCCTGAAGAAGCGGCCTGCCTCGGCCAA
  GTCCCGCAGGGAGTTTGACGAGATCGAGCTGGCCTACCGTCGCCGACCGCCCCGCTCCCCTGACCACAAG
  CGCTACTTCAGGGACAAGGAAGGGCTACGGGACTTCTACCTGGACCAGTTCCGAACAAAGGAGAACTCAC
  CCCACTGGGAGCACGTAGACCTGACCGACATCTACAAGGAGCGGAGTGATGACTTTAAGCGCGACTCCAT
  CAGCGGAGGAGGGCCCTGTACCAACAGGTCTCACATCAAGCACGGGACGGGCGACAAACACGGCGTGGTC
  AGCGGGGTACCTGCACCTTGGGAGAAGAACCTGACCAACGTGGAGTGGGAGGACCGGTCCGGGGGCAACT
  TCTGCCGCAGCTGTCCCTCCAAGCTGCACAACTACTCCACGACGGTGACGGGTCAGAACTCGGGCAGGCA
  GGCGTGCATCCGGTGTGAGGCTTGCAAGAAAGCAGGCAACCTGTATGACATCAGTGAGGACAACTCCCTG
  CAGGAACTGGACCAGCCGGCTGCCCCAGTGGCGGTGACGTCAAACGCCTCCACCACTAAGTACCCTCAGA
  GCCCGACTAATTCCAAGGCCCAGAAGAAGAACCGGAACAAACTGCGCCGGCAGCACTCCTACGACACCTT
  CGTGGACCTGCAGAAGGAAGAAGCCGCCCTGGCCCCGCGCAGCGTAAGCCTGAAAGACAAGGGCCGATTC
  ATGGATGGGAGCCCCTACGCCCACATGTTTGAGATGTCAGCTGGCGAGAGCACCTTTGCCAACAACAAGT
  CCTCAGTGCCCACTGCCGGACATCACCACCACAACAACCCCGGCGGCGGGTACATGCTCAGCAAGTCGCT
  CTACCCTGACCGGGTCACGCAAAACCCTTTCATCCCCACTTTTGGGGACGACCAGTGCTTGCTCCATGGC
  AGCAAATCCTACTTCTTCAGGCAGCCCACGGTGGCGGGGGCGTCGAAAGCCAGGCCGGACTTCCGGGCCC
  TTGTCACCAACAAGCCGGTGGTCTCGGCCCTTCATGGGGCCGTGCCAGCCCGTTTCCAGAAGGACATCTG
  TATAGGGAACCAGTCCAACCCCTGTGTGCCTAACAACAAAAACCCCAGGGCTTTCAATGGCTCCAGCAAT
  GGGCATGTTTATGAGAAACTTTCTAGTATTGAGTCTGATGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223623 representing NM_000834
  Red=Cloning site Green=Tags(s)
  
  MKPRAECCSPKFWLVLAVLAVSGSRARSQKSPPSIGIAVILVGTSDEVAIKDAHEKDDFHHLSVVPRVEL
  VAMNETDPKSIITRICDLMSDRKIQGVVFADDTDQEAIAQILDFISAQTLTPILGIHGGSSMIMADKDES
  SMFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYFPGYQDFVNKIRSTIENSFVGWELEEVLLLDMSLD
  DGDSKIQNQLKKLQSPIILLYCTKEEATYIFEVANSVGLTGYGYTWIVPSLVAGDTDTVPAEFPTGLISV
  SYDEWDYGLPARVRDGIAIITTAASDMLSEHSFIPEPKSSCYNTHEKRIYQSNMLNRYLINVTFEGRNLS
  FSEDGYQMHPKLVIILLNKERKWERVGKWKDKSLQMKYYVWPRMCPETEEQEDDHLSIVTLEEAPFVIVE
  SVDPLSGTCMRNTVPCQKRIVTENKTDEEPGYIKKCCKGFCIDILKKISKSVKFTYDLYLVTNGKHGKKI
  NGTWNGMIGEVVMKRAYMAVGSLTINEERSEVVDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWV
  MMFVMLLIVSAVAVFVFEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKI
  MVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVSGLSDKKFQRPNDFSPPFRFGTVPNGSTERNIRNNYA
  EMHAYMGKFNQRGVDDALLSLKTGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGKVFASTGYGIAIQKDS
  GWKRQVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQLDIDNMAGVFYMLGAAMALSLITFICEH
  LFYWQFRHCFMGVCSGKPGMVFSISRGIYSCIHGVAIEERQSVMNSPTATMNNTHSNILRLLRTAKNMAN
  LSGVNGSPQSALDFIRRESSVYDISEHRRSFTHSDCKSYNNPPCEENLFSDYISEVERTFGNLQLKDSNV
  YQDHYHHHHRPHSIGSASSIDGLYDCDNPPFTTQSRSISKKPLDIGLPSSKHSQLSDLYGKFSFKSDRYS
  GHDDLIRSDVSDISTHTVTYGNIEGNAAKRRKQQYKDSLKKRPASAKSRREFDEIELAYRRRPPRSPDHK
  RYFRDKEGLRDFYLDQFRTKENSPHWEHVDLTDIYKERSDDFKRDSISGGGPCTNRSHIKHGTGDKHGVV
  SGVPAPWEKNLTNVEWEDRSGGNFCRSCPSKLHNYSTTVTGQNSGRQACIRCEACKKAGNLYDISEDNSL
  QELDQPAAPVAVTSNASTTKYPQSPTNSKAQKKNRNKLRRQHSYDTFVDLQKEEAALAPRSVSLKDKGRF
  MDGSPYAHMFEMSAGESTFANNKSSVPTAGHHHHNNPGGGYMLSKSLYPDRVTQNPFIPTFGDDQCLLHG
  SKSYFFRQPTVAGASKARPDFRALVTNKPVVSALHGAVPARFQKDICIGNQSNPCVPNNKNPRAFNGSSN
  GHVYEKLSSIESDV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000834
• ORF Size: 4452 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000834.2, NP_000825.1
• RefSeq Size: 6240 bp
• RefSeq ORF: 4455 bp
• Locus ID: 2904
• UniProt ID: Q13224
• Cytogenetics: 12p13.1
• Domains: lig_chan
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Huntington's disease, Long-term potentiation, Neuroactive ligand-receptor interaction, Systemic lupus erythematosus
• Gene Summary: This gene encodes a member of the N-methyl-D-aspartate (NMDA) receptor family within the ionotropic glutamate receptor superfamily. The encoded protein is a subunit of the NMDA receptor ion channel which acts as an agonist binding site for glutamate. The NMDA receptors mediate a slow calcium-permeable component of excitatory synaptic transmission in the central nervous system. The NMDA receptors are heterotetramers of seven genetically encoded, differentially expressed subunits including NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The early expression of this gene in development suggests a role in brain development, circuit formation, synaptic plasticity, and cellular migration and differentiation. Naturally occurring mutations within this gene are associated with neurodevelopmental disorders including autism spectrum disorder, attention deficit hyperactivity disorder, epilepsy, and schizophrenia. [provided by RefSeq, Aug 2017]