NMDAR2B (GRIN2B) (NM_000834) Human Untagged Clone

CAT#: SC119642

GRIN2B (untagged)-Human glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NMDAR2B
• Synonyms: DEE27; EIEE27; GluN2B; hNR3; MRD6; NMDAR2B; NR2B; NR3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000834, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGCCCAGAGCGGAGTGCTGTTCTCCCAAGTTCTGGTTGGTGTTGGCCGTCCTGGCCGTGTCAGGCA
  GCAGAGCTCGTTCTCAGAAGAGCCCCCCCAGCATTGGCATTGCTGTCATCCTCGTGGGCACTTCCGACGA
  GGTGGCCATCAAGGATGCCCACGAGAAAGATGATTTCCACCATCTCTCCGTGGTACCCCGGGTGGAACTG
  GTAGCCATGAATGAGACCGACCCAAAGAGCATCATCACCCGCATCTGTGATCTCATGTCTGACCGGAAGA
  TCCAGGGGGTGGTGTTTGCTGATGACACAGACCAGGAAGCCATCGCCCAGATCCTCGATTTCATTTCAGC
  ACAGACTCTCACCCCCATCCTGGGCATCCACGGGGGCTCCTCTATGATAATGGCAGATAAGGATGAATCC
  TCCATGTTCTTCCAGTTTGGCCCATCAATTGAACAGCAAGCTTCCGTAATGCTCAACATCATGGAAGAAT
  ATGACTGGTACATCTTTTCTATCGTCACCACCTATTTCCCTGGCTACCAGGACTTTGTAAACAAGATCCG
  CAGCACCATTGAGAATAGCTTTGTGGGCTGGGAGCTAGAGGAGGTCCTCCTACTGGACATGTCCCTGGAC
  GATGGAGATTCTAAGATCCAGAATCAGCTCAAGAAACTTCAAAGCCCCATCATTCTTCTTTACTGTACCA
  AGGAAGAAGCCACCTACATCTTTGAAGTGGCCAACTCAGTAGGGCTGACTGGCTATGGCTACACGTGGAT
  CGTGCCCAGTCTGGTGGCAGGGGATACAGACACAGTGCCTGCGGAGTTCCCCACTGGGCTCATCTCTGTA
  TCATATGATGAATGGGACTATGGCCTCCCCGCCAGAGTGAGAGATGGAATTGCCATAATCACCACTGCTG
  CTTCTGACATGCTGTCTGAGCACAGCTTCATCCCTGAGCCCAAAAGCAGTTGTTACAACACCCACGAGAA
  GAGAATCTACCAGTCCAATATGCTAAATAGGTATCTGATCAATGTCACTTTTGAGGGGAGGAATTTGTCC
  TTCAGTGAAGATGGCTACCAGATGCACCCGAAACTGGTGATAATTCTTCTGAACAAGGAGAGGAAGTGGG
  AAAGGGTGGGGAAGTGGAAAGACAAGTCCCTGCAGATGAAGTACTATGTGTGGCCCCGAATGTGTCCAGA
  GACTGAAGAGCAGGAGGATGACCATCTGAGCATTGTGACCCTGGAGGAGGCACCATTTGTCATTGTGGAA
  AGTGTGGACCCTCTGAGTGGAACCTGCATGAGGAACACAGTCCCCTGCCAAAAACGCATAGTCACTGAGA
  ATAAAACAGACGAGGAGCCGGGTTACATCAAAAAATGCTGCAAGGGGTTCTGTATTGACATCCTTAAGAA
  AATTTCTAAATCTGTGAAGTTCACCTATGACCTTTACCTGGTTACCAATGGCAAGCATGGGAAGAAAATC
  AATGGAACCTGGAATGGTATGATTGGAGAGGTGGTCATGAAGAGGGCCTACATGGCAGTGGGCTCACTCA
  CCATCAATGAGGAACGATCGGAGGTGGTCGACTTCTCTGTGCCCTTCATAGAGACAGGCATCAGTGTCAT
  GGTGTCACGCAGCAATGGGACTGTCTCACCTTCTGCCTTCTTAGAGCCATTCAGCGCTGACGTATGGGTG
  ATGATGTTTGTGATGCTGCTCATCGTCTCAGCCGTGGCTGTCTTTGTCTTTGAGTACTTCAGCCCTGTGG
  GTTATAACAGGTGCCTCGCTGATGGCAGAGAGCCTGGTGGACCCTCTTTCACCATCGGCAAAGCTATTTG
  GTTGCTCTGGGGTCTGGTGTTTAACAACTCCGTACCTGTGCAGAACCCAAAGGGGACCACCTCCAAGATC
  ATGGTGTCAGTGTGGGCCTTCTTTGCTGTCATCTTCCTGGCCAGCTACACTGCCAACTTAGCTGCCTTCA
  TGATCCAAGAGGAATATGTGGACCAGGTTTCTGGCCTGAGCGACAAAAAGTTCCAGAGACCTAATGACTT
  CTCACCCCCTTTCCGCTTTGGGACCGTGCCCAACGGCAGCACAGAGAGAAATATTCGCAATAACTATGCA
  GAAATGCATGCCTACATGGGAAAGTTCAACCAGAGGGGTGTAGATGATGCATTGCTCTCCCTGAAAACAG
  GGAAACTGGATGCCTTCATCTATGATGCAGCAGTGCTGAACTATATGGCAGGCAGAGATGAAGGCTGCAA
  GCTGGTGACCATTGGCAGTGGGAAGGTCTTTGCTTCCACTGGCTATGGCATTGCCATCCAAAAAGATTCT
  GGGTGGAAGCGCCAGGTGGACCTTGCTATCCTGCAGCTCTTTGGAGATGGGGAGATGGAAGAACTGGAAG
  CTCTCTGGCTCACTGGCATTTGTCACAATGAGAAGAATGAGGTCATGAGCAGCCAGCTGGACATTGACAA
  CATGGCAGGGGTCTTCTACATGTTGGGGGCGGCCATGGCTCTCAGCCTCATCACCTTCATCTGCGAACAC
  CTTTTCTATTGGCAGTTCCGACATTGCTTTATGGGTGTCTGTTCTGGCAAGCCTGGCATGGTCTTCTCCA
  TCAGCAGAGGTATCTACAGCTGCATCCATGGGGTGGCGATCGAGGAGCGCCAGTCTGTAATGAACTCCCC
  CACCGCAACCATGAACAACACACACTCCAACATCCTGCGCCTGCTGCGCACGGCCAAGAACATGGCTAAC
  CTGTCTGGTGTGAATGGCTCACCGCAGAGCGCCCTGGACTTCATCCGACGGGAGTCATCCGTCTATGACA
  TCTCAGAGCACCGCCGCAGCTTCACGCATTCTGACTGCAAATCCTACAACAACCCGCCCTGTGAGGAGAA
  CCTCTTCAGTGACTACATCAGTGAGGTAGAGAGAACGTTCGGGAACCTGCAGCTGAAGGACAGCAACGTG
  TACCAAGATCACTACCACCATCACCACCGGCCCCATAGTATTGGCAGTGCCAGCTCCATCGATGGGCTCT
  ACGACTGTGACAACCCACCCTTCACCACCCAGTCCAGGTCCATCAGCAAGAAGCCCCTGGACATCGGCCT
  CCCCTCCTCCAAGCACAGCCAGCTCAGTGACCTGTACGGCAAATTCTCCTTCAAGAGCGACCGCTACAGT
  GGCCACGACGACTTGATCCGCTCCGATGTCTCTGACATCTCAACCCACACCGTCACCTATGGGAACATCG
  AGGGCAATGCCGCCAAGAGGCGTAAGCAGCAATATAAGGACAGCCTGAAGAAGCGGCCTGCCTCGGCCAA
  GTCCCGCAGGGAGTTTGACGAGATCGAGCTGGCCTACCGTCGCCGACCGCCCCGCTCCCCTGACCACAAG
  CGCTACTTCAGGGACAAGGAAGGGCTACGGGACTTCTACCTGGACCAGTTCCGAACAAAGGAGAACTCAC
  CCCACTGGGAGCACGTAGACCTGACCGACATCTACAAGGAGCGGAGTGATGACTTTAAGCGCGACTCCGT
  CAGCGGAGGAGGGCCCTGTACCAACAGGTCTCACATCAAGCACGGGACGGGCGACAAACACGGCGTGGTC
  AGCGGGGTACCTGCACCTTGGGAGAAGAACCTGACCAACGTGGAGTGGGAGGACCGGTCCGGGGGCAACT
  TCTGCCGCAGCTGTCCCTCCAAGCTGCACAACTACTCCACGACGGTGACGGGTCAGAACTCGGGCAGGCA
  GGCGTGCATCCGGTGTGAGGCTTGCAAGAAAGCAGGCAACCTGTATGACATCAGTGAGGACAACTCCCTG
  CAGGAACTGGACCAGCCGGCTGCCCCAGTGGCGGTGACGTCAAACGCCTCCACCACTAAGTACCCTCAGA
  GCCCGACTAATTCCAAGGCCCAGAAGAAGAACCGGAACAAACTGCGCCGGCAGCACTCCTACGACACCTT
  CGTGGACCTGCAGAAGGAAGAAGCCGCCCTGGCCCCGCGCAGCGTAAGCCTGAAAGACAAGGGCCGATTC
  ATGGATGGGAGCCCCTACGCCCACATGTTTGAGATGTCAGCTGGCGAGAGCACCTTTGCCAACAACAAGT
  CCTCAGTGCCCACTGCCGGACATCACCACCACAACAACCCCGGCGGCGGGTACATGCTCAGCAAGTCGCT
  CTACCCTGACCGGGTCACGCAAAACCCTTTCATCCCCACTTTTGGGGACGACCAGTGCTTGCTCCATGGC
  AGCAAATCCTACTTCTTCAGGCAGCCCACGGTGGCGGGGGCGTCGAAAGCCAGGCCGGACTTCCGGGCCC
  TTGTCACCAACAAGCCGGTGGTCTCGGCCCTTCATGGGGCCGTGCCAGCCCGTTTCCAGAAGGACATCTG
  TATAGGGAACCAGTCCAACCCCTGTGTGCCTAACAACAAAAACCCCAGGGCTTTCAATGGCTCCAGCAAT
  GGGCATGTTTATGAGAAACTTTCTAGTATTGAGTCTGATGTCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000834 unedited
  GCATTTGTATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGTGGACATTCCCA
  ACATGCTCACTCCCTTAATCTGTCCGTCTAGAGGTTTGGCTTCTACAAACCAAGGGAGTC
  GACGAGTTGAAGATGAAGCCCAGAGCGGAGTGCTGTTCTCCCAAGTTCTGGTTGGTGTTG
  GCCGTCCTGGCCGTGTCAGGCAGCAGAGCTCGTTCTCAGAAGAGCCCCCCCAGCATTGGC
  ATTGCTGTCATCCTCGTGGGCACTTCCGACGAGGTGGCCATCAAGGATGCCCACGAGAAA
  GATGATTTCCACCATCTCTCCGTGGTACCCCGGGTGGAACTGGTAGCCATGAATGAGACC
  GACCCAAAGAGCATCATCACCCGCATCTGTGATCTCATGTCTGACCGGACGATCCAGGGG
  GTGGTGTTTGCTGATGACACAGACCAGGAAGCCATCGCCCAGATCCTCGATTTCATTTCA
  GCACAGACTCTCACCCCGATCCTGGGCATCCACGGGGGCTCCTCTATGATAATGCTAGAT
  AAGGATGAATCCTCCATGTTCTTCCAGTTTGGCCCATCAATTGAACAGCAAGCTTTCGTA
  ATGCTCAACATCATGGAAGAATATGACTGGTACATCTTTTCTATCGTCACCACCTATTTC
  CCTGGCTACCAGGACTTTGTAAACANGATCCGCAGCACCATTGAGAATAGCTTTGTGGGC
  CTGGAGCTAGAGGGAGTCCTCCTACTGGACATGTCCCTGGACGATGGAGATTCTAAGATC
  CAGCATCAGCTCAAGAAACTTCAAAGCCCCATCATTCTTCCTTACTGTACCAGAGAAGAG
  GCCCCTTACTCTTTTGAGTGGCCAACTCAGNAAGGCTGACTGGCTATTGCTACACGTGGA
  TCGTGCCCATCTGGTGGCAGGGGAATCAGACCCGTTGCTGCGGAGTTCCCACTGGCTCAT
  CTTGTTCATTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000834 unedited
  ATGGACCGGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATTCCTCATGGT
  ACAATCTGAGAGGGAAACAAGGTGTTGCAGGAAGGTAGAAGGGCTTCTACCAAGAGTGTT
  CACTCTGGGCTCAAGTCACATTCCCAGGGAGCCAGGCCCAAAGAGGTGGTGGAGGGTCCC
  TCTCTGCCCCCGTCTCCCATTTCCAAGTCTCTTCCCCTCTTTTCGTACCTCCAGAGCTGC
  ACTCATGGAGTGCAGCTCATTTCTCTTAAATGTCAAGCTCATTTCTCTTAACTGTCTGCT
  CTGTAGCAGTTGTAATCAGTTTTGGGGGGAAGAAGCCTGCTCGTCTAGCTTCAGGTTAAG
  TAAGAAGGGATCAAAGCATTGGAAACATCCCCCTCTCTCACTTACCCTACCATACACGAC
  TCCTTACTTCTTCAAAGGTGGCTTCGTTCTTCCCCAAACCTTGGCCAGTCGAGAGTCTAA
  ATGCTCAAAAGAGCTTTGTAGAATCTTTTGCTCCCAAGTGTGGGCAAAAGAATCATGGCT
  GTGAAAATTCGGCTTCCCTTTAGCCTTCCTGGAAAGTTCTTGGTTCAAACTGGTTGAAAC
  AATTGCCAACCACTGAGGACTTGTTTATGTACCTTGCTCTTGCAGGATTGCTCACCGCCT
  TTTTTTGTTTAGACCACAGAATCTTCTTTATCAAGAATTCTCATTCTCCTTATTTCACAA
  GATGAACGGAGACCCAGCCAAAATTCAGGCAATACATGGCCATCTCTGTCTGTATACTAG
  CCAAACAATCACACCTGCTTGGGGATTTCCAAAGAACCATCCCACAGAGCCAGGGTTGCC
  CTCTTCTTGAGGCACCTGGATAACACATGCGCCCAAGTCCCGCATGAACCCCACATGCCC
  GCCAAAATTCCACTGCTCTTGGNCCCCAGGACCGGGGGAACACTTACAACCGCACCTTTC
  ACGCGGCCACAGACCCACCTGTCCACCAGCGACTCTGTGTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000834
• Insert Size: 6250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000834.2, NP_000825.1
• RefSeq Size: 6240 bp
• RefSeq ORF: 4455 bp
• Locus ID: 2904
• UniProt ID: Q13224
• Cytogenetics: 12p13.1
• Domains: lig_chan
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Huntington's disease, Long-term potentiation, Neuroactive ligand-receptor interaction, Systemic lupus erythematosus
• Gene Summary: This gene encodes a member of the N-methyl-D-aspartate (NMDA) receptor family within the ionotropic glutamate receptor superfamily. The encoded protein is a subunit of the NMDA receptor ion channel which acts as an agonist binding site for glutamate. The NMDA receptors mediate a slow calcium-permeable component of excitatory synaptic transmission in the central nervous system. The NMDA receptors are heterotetramers of seven genetically encoded, differentially expressed subunits including NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The early expression of this gene in development suggests a role in brain development, circuit formation, synaptic plasticity, and cellular migration and differentiation. Naturally occurring mutations within this gene are associated with neurodevelopmental disorders including autism spectrum disorder, attention deficit hyperactivity disorder, epilepsy, and schizophrenia. [provided by RefSeq, Aug 2017]