T (NM_003181) Human Tagged ORF Clone

CAT#: RC218530

T (Myc-DDK-tagged)-Human T, brachyury homolog (mouse) (T)

Product Images

Circular map for RC218530

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  Western blot validation of overexpression lysate (Cat# LY401103) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218530 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TBXT protein (Cat# TP318530). The protein was produced from HEK293T cells transfected with TBXT cDNA clone (Cat# RC218530) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: T
• Synonyms: SAVA; T; TFT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218530 representing NM_003181
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCCCTGGCACCGAGAGCGCGGGAAAGAGCCTGCAGTACCGAGTGGACCACCTGCTGAGCGCCG
  TGGAGAATGAGCTGCAGGCGGGCAGCGAGAAGGGCGACCCCACAGAGCGCGAACTGCGCGTGGGCCTGGA
  GGAGAGCGAGCTGTGGCTGCGCTTCAAGGAGCTCACCAATGAGATGATCGTGACCAAGAACGGCAGGAGG
  ATGTTTCCGGTGCTGAAGGTGAACGTGTCTGGCCTGGACCCCAACGCCATGTACTCCTTCCTGCTGGACT
  TCGTGGCGGCGGACAACCACCGCTGGAAGTACGTGAACGGGGAATGGGTGCCGGGGGGCAAGCCGGAGCC
  GCAGGCGCCCAGCTGCGTCTACATCCACCCCGACTCGCCCAACTTCGGGGCCCACTGGATGAAGGCTCCC
  GTCTCCTTCAGCAAAGTCAAGCTCACCAACAAGCTCAACGGAGGGGGCCAGATCATGCTGAACTCCTTGC
  ATAAGTATGAGCCTCGAATCCACATAGTGAGAGTTGGGGGTCCACAGCGCATGATCACCAGCCACTGCTT
  CCCTGAGACCCAGTTCATAGCGGTGACTGCTTATCAGAACGAGGAGATCACAGCTCTTAAAATTAAGTAC
  AATCCATTTGCAAAAGCTTTCCTTGATGCAAAGGAAAGAAGTGATCACAAAGAGATGATGGAGGAACCCG
  GAGACAGCCAGCAACCTGGGTACTCCCAATCAGGGGGGTGGCTTCTTCCTGGAACCAGCACCCTGTGTCC
  ACCTGCAAATCCTCATCCTCAGTTTGGAGGTGCCCTCTCCCTCCCCTCCACGCACAGCTGTGACAGGTAC
  CCAACCCTGAGGAGCCACCGGTCCTCACCCTACCCCAGCCCCTATGCTCATCGGAACAATTCTCCAACCT
  ATTCTGACAACTCACCTGCATGTTTATCCATGCTGCAATCCCATGACAATTGGTCCAGCCTTGGAATGCC
  TGCCCATCCCAGCATGCTCCCCGTGAGCCACAATGCCAGCCCACCTACCAGCTCCAGTCAGTACCCCAGC
  CTGTGGTCTGTGAGCAACGGCGCCGTCACCCCGGGCTCCCAGGCAGCAGCCGTGTCCAACGGGCTGGGGG
  CCCAGTTCTTCCGGGGCTCCCCCGCGCACTACACACCCCTCACCCATCCGGTCTCGGCGCCCTCTTCCTC
  GGGATCCCCACTGTACGAAGGGGCGGCCGCGGCCACAGACATCGTGGACAGCCAGTACGACGCCGCAGCC
  CAAGGCCGCCTCATAGCCTCATGGACACCTGTGTCGCCACCTTCCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218530 representing NM_003181
  Red=Cloning site Green=Tags(s)
  
  MSSPGTESAGKSLQYRVDHLLSAVENELQAGSEKGDPTERELRVGLEESELWLRFKELTNEMIVTKNGRR
  MFPVLKVNVSGLDPNAMYSFLLDFVAADNHRWKYVNGEWVPGGKPEPQAPSCVYIHPDSPNFGAHWMKAP
  VSFSKVKLTNKLNGGGQIMLNSLHKYEPRIHIVRVGGPQRMITSHCFPETQFIAVTAYQNEEITALKIKY
  NPFAKAFLDAKERSDHKEMMEEPGDSQQPGYSQSGGWLLPGTSTLCPPANPHPQFGGALSLPSTHSCDRY
  PTLRSHRSSPYPSPYAHRNNSPTYSDNSPACLSMLQSHDNWSSLGMPAHPSMLPVSHNASPPTSSSQYPS
  LWSVSNGAVTPGSQAAAVSNGLGAQFFRGSPAHYTPLTHPVSAPSSSGSPLYEGAAAATDIVDSQYDAAA
  QGRLIASWTPVSPPSM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003181
• ORF Size: 1308 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003181.4
• RefSeq Size: 2518 bp
• RefSeq ORF: 1308 bp
• Locus ID: 6862
• UniProt ID: O15178
• Cytogenetics: 6q27
• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• MW: 47.3 kDa
• Gene Summary: The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Variation in this gene was associated with susceptibility to neural tube defects and chordoma. A mutation in this gene was found in a family with sacral agenesis with vertebral anomalies. [provided by RefSeq, Sep 2018]