FGF4 (NM_002007) Human Tagged ORF Clone
FGF4 (Myc-DDK-tagged)-Human fibroblast growth factor 4 (FGF4)
|Product Name||FGF4 (NM_002007) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||FGF-4; HBGF-4; HST; HST-1; HSTF-1; HSTF1; K-FGF; KFGF|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC217058 representing NM_002007
Red=Cloning site Blue=ORF Green=Tags(s)
>RC217058 representing NM_002007
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||618 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1219 bp|
|RefSeq ORF||621 bp|
|Protein Families||Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway, Transmembrane|
|Protein Pathways||MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton|
|Gene Summary||The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its oncogenic transforming activity. This gene and FGF3, another oncogenic growth factor, are located closely on chromosome 11. Co-amplification of both genes was found in various kinds of human tumors. Studies on the mouse homolog suggested a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. [provided by RefSeq, Jul 2008]|
|cDNA Clone Resources|
|RC217058L1||Lenti ORF clone of Human fibroblast growth factor 4 (FGF4), Myc-DDK-tagged||
|RC217058L2||Lenti ORF clone of Human fibroblast growth factor 4 (FGF4), mGFP tagged||
|RC217058L3||Lenti ORF clone of Human fibroblast growth factor 4 (FGF4), Myc-DDK-tagged||
|RC217058L4||Lenti ORF clone of Human fibroblast growth factor 4 (FGF4), mGFP tagged||
|SC303106||FGF4 (untagged)-Human fibroblast growth factor 4 (FGF4)||
|RG217058||FGF4 (GFP-tagged) - Human fibroblast growth factor 4 (FGF4)||