SRP72 (NM_006947) Human Tagged ORF Clone

CAT#: RC214727

SRP72 (Myc-DDK-tagged)-Human signal recognition particle 72kDa (SRP72)

Product Images

Circular map for RC214727

• 

  Western blot validation of overexpression lysate (Cat# LY416310) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214727 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SRP72
• Synonyms: BMFF; BMFS1; HEL103
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214727 representing NM_006947
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGCGGCGGCAGCGGGGGGGTGTCAGTACCTGCGCTGTGGAGTGAAGTGAACCGGTATGGCCAGA
  ACGGCGACTTCACGCGCGCTCTCAAGACCGTCAATAAGATACTACAGATCAACAAAGATGACGTAACTGC
  CCTGCATTGTAAAGTGGTATGCCTTATCCAGAATGGAAGTTTCAAGGAAGCTTTGAATGTCATCAATACT
  CACACCAAAGTGTTAGCCAATAACTCTCTCTCCTTTGAAAAGGCATATTGCGAGTACAGGCTGAACAGAA
  TTGAGAATGCCTTGAAGACAATAGAAAGTGCCAACCAGCAGACAGACAAACTGAAGGAGCTTTATGGACA
  AGTGTTATATCGTTTGGAACGCTATGATGAATGCTTAGCAGTGTATAGAGATCTCGTCCGAAACTCCCAA
  GATGATTATGATGAGGAGAGGAAAACAAACCTTTCAGCAGTTGTTGCAGCTCAAAGCAATTGGGAAAAAG
  TGGTTCCAGAGAACCTGGGCCTCCAAGAAGGCACACATGAGCTGTGCTACAACACTGCATGTGCACTGAT
  AGGCCAAGGCCAGCTGAACCAGGCCATGAAAATCCTACAAAAAGCTGAAGATCTTTGCCGCCGTTCATTA
  TCAGAAGACACTGATGGGACTGAGGAAGACCCACAGGCAGAACTGGCCATCATTCATGGTCAGATGGCTT
  ATATTCTGCAGCTTCAGGGTCGAACAGAGGAGGCTTTGCAACTTTACAATCAAATAATAAAACTAAAACC
  AACAGATGTGGGATTACTAGCTGTAATTGCAAATAACATCATTACCATTAACAAGGACCAAAATGTCTTT
  GACTCCAAGAAGAAAGTGAAATTAACCAATGCGGAAGGAGTAGAGTTTAAGCTTTCCAAGAAACAACTAC
  AAGCTATAGAATTTAACAAAGCTTTACTTGCTATGTACACAAACCAGGCTGAACAATGCCGCAAAATATC
  TGCCAGTTTACAGTCCCAAAGTCCCGAGCATCTCTTACCTGTGTTAATCCAAGCTGCCCAGCTCTGCCGT
  GAAAAGCAGCACACAAAAGCAATAGAGCTGCTTCAGGAATTTTCAGATCAGCATCCAGAAAATGCAGCTG
  AAATTAAGCTGACCATGGCACAGTTGAAAATTTCTCAAGGTAATATTTCTAAAGCATGTCTAATATTGAG
  AAGCATAGAGGAGTTAAAGCATAAACCAGGCATGGTATCTGCATTAGTTACCATGTATAGCCATGAAGAA
  GATATTGATAGTGCCATTGAGGTCTTCACACAAGCTATCCAGTGGTATCAAAACCATCAGCCAAAATCTC
  CTGCTCATTTGTCCTTGATAAGAGAAGCTGCAAACTTCAAACTCAAATATGGGCGGAAGAAGGAGGCAAT
  TAGTGACCTACAACAGCTGTGGAAACAAAATCCAAAAGATATTCACACCCTGGCACAGCTTATTTCTGCT
  TACTCACTTGTAGATCCAGAGAAAGCCAAAGCTCTTAGTAAACACTTGCCATCGTCAGATAGTATGTCTC
  TAAAAGTAGATGTTGAGGCTCTTGAAAATTCTGCTGGTGCTACATACATTCGGAAGAAGGGTGGAAAAGT
  TACTGGAGATAGTCAACCAAAGGAACAAGGACAGGGAGATTTGAAAAAGAAGAAAAAGAAAAAGAAGGGA
  AAATTGCCTAAGAATTATGACCCAAAAGTTACCCCAGATCCAGAAAGATGGCTGCCAATGCGAGAACGTT
  CTTACTACCGGGGAAGAAAGAAGGGTAAAAAGAAGGATCAGATTGGAAAAGGGACCCAGGGAGCAACTGC
  AGGAGCTTCATCTGAACTGGATGCCAGTAAAACTGTGAGCAGCCCACCCACCTCCCCAAGACCTGGCAGT
  GCTGCAACAGTATCTGCCTCTACAAGTAACATCATACCCCCAAGACACCAGAAACCTGCAGGGGCTCCAG
  CAACAAAAAAGAAACAGCAACAGAAAAAGAAGAAAGGTGGAAAAGGTGGCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214727 representing NM_006947
  Red=Cloning site Green=Tags(s)
  
  MASGGSGGVSVPALWSEVNRYGQNGDFTRALKTVNKILQINKDDVTALHCKVVCLIQNGSFKEALNVINT
  HTKVLANNSLSFEKAYCEYRLNRIENALKTIESANQQTDKLKELYGQVLYRLERYDECLAVYRDLVRNSQ
  DDYDEERKTNLSAVVAAQSNWEKVVPENLGLQEGTHELCYNTACALIGQGQLNQAMKILQKAEDLCRRSL
  SEDTDGTEEDPQAELAIIHGQMAYILQLQGRTEEALQLYNQIIKLKPTDVGLLAVIANNIITINKDQNVF
  DSKKKVKLTNAEGVEFKLSKKQLQAIEFNKALLAMYTNQAEQCRKISASLQSQSPEHLLPVLIQAAQLCR
  EKQHTKAIELLQEFSDQHPENAAEIKLTMAQLKISQGNISKACLILRSIEELKHKPGMVSALVTMYSHEE
  DIDSAIEVFTQAIQWYQNHQPKSPAHLSLIREAANFKLKYGRKKEAISDLQQLWKQNPKDIHTLAQLISA
  YSLVDPEKAKALSKHLPSSDSMSLKVDVEALENSAGATYIRKKGGKVTGDSQPKEQGQGDLKKKKKKKKG
  KLPKNYDPKVTPDPERWLPMRERSYYRGRKKGKKKDQIGKGTQGATAGASSELDASKTVSSPPTSPRPGS
  AATVSASTSNIIPPRHQKPAGAPATKKKQQQKKKKGGKGGW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006947
• ORF Size: 2013 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006947.4
• RefSeq Size: 3852 bp
• RefSeq ORF: 2016 bp
• Locus ID: 6731
• UniProt ID: O76094
• Cytogenetics: 4q12
• Domains: TPR
• Protein Pathways: Protein export
• MW: 74.4 kDa
• Gene Summary: This gene encodes the 72 kDa subunit of the signal recognition particle (SRP), a ribonucleoprotein complex that mediates the targeting of secretory proteins to the endoplasmic reticulum (ER). The SRP complex consists of a 7S RNA and 6 protein subunits: SRP9, SRP14, SRP19, SRP54, SRP68, and SRP72, that are bound to the 7S RNA as monomers or heterodimers. SRP has at least 3 distinct functions that can be associated with the protein subunits: signal recognition, translational arrest, and ER membrane targeting by interaction with the docking protein. Mutations in this gene are associated with familial bone marrow failure. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]