OXA1L (NM_005015) Human Tagged ORF Clone

CAT#: RC213574

OXA1L (Myc-DDK-tagged)-Human oxidase (cytochrome c) assembly 1-like (OXA1L), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC213574

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OXA1L
• Synonyms: OXA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213574 representing NM_005015
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAACGTGGCTTTACAGATTTTTACCCACTTCAAATATGGCCGCCAAGCTCCGTTCTCTTTTACCGC
  CTGATCTGCGGCTACAATTCTGGCTTCATGCCCGCCTCCAAAAGTGCTTCCTCTCGAGGGGTTGTGGTTC
  TTACTGCGCAGGCGCAAAAGCAAGTCCTCTTCCGGGCAAAATGGCGATGGGACTAATGTGCGGACGCCGG
  GAGCTTCTGCGCTTGCTACAGTCCGGGCGTCGGGTCCACAGCGTCGCAGGGCCCTCGCAATGGCTTGGGA
  AACCGCTGACCACACGGCTCCTATTCCCAGCAGCCCCGTGCTGCTGTCGCCCACACTACCTCTTCCTTGC
  GGCTTCCGGCCCCCGCAGCCTCAGTACCTCTGCTATCTCTTTTGCAGAAGTCCAGGTTCAGGCCCCTCCT
  GTTGTTGCTGCAACTCCCTCACCCACAGCAGTACCTGAGGTGGCTTCTGGAGAGACTGCAGATGTAGTCC
  AAACTGCTGCAGAGCAGAGCTTCGCTGAACTGGGGCTGGGGTCATACACCCCAGTGGGACTGATCCAGAA
  TTTACTGGAATTTATGCATGTTGATCTGGGCCTACCTTGGTGGGGGGCCATTGCTGCATGTACAGTCTTT
  GCCCGCTGCCTGATTTTTCCTCTCATCGTGACGGGCCAGCGAGAGGCAGCCAGGATCCACAATCACTTGC
  CAGAGATCCAGAAGTTTTCCAGTCGAATCAGAGAGGCCAAGTTAGCAGGAGACCATATTGAGTATTACAA
  GGCTTCCTCGGAGATGGCACTTTACCAGAAAAAACATGGTATTAAACTCTATAAACCTCTCATTCTCCCT
  GTGACTCAGGCCCCAATCTTCATCTCCTTCTTCATTGCTTTGAGAGAGATGGCCAACCTTCCTGTGCCCA
  GCCTGCAGACAGGTGGCCTCTGGTGGTTCCAGGATCTCACGGTATCCGATCCCATCTACATATTACCACT
  GGCAGTCACTGCTACAATGTGGGCTGTTCTTGAGCTAGGTGCTGAGACAGGTGTGCAAAGTTCTGACCTT
  CAGTGGATGAGAAATGTCATCAGAATGATGCCCCTGATAACCTTGCCCATAACCATGCATTTCCCCACGG
  CAGTGTTTATGTACTGGCTCTCCTCCAATTTGTTTTCCCTGGTCCAAGTATCCTGTCTCCGGATTCCAGC
  AGTACGCACTGTACTTAAAATCCCCCAGCGTGTTGTACATGACCTGGACAAATTACCTCCACGGGAAGGC
  TTCCTAGAGAGCTTCAAAAAAGGCTGGAAAAATGCTGAAATGACGCGTCAGCTGCGAGAGCGTGAACAAC
  GCATGCGGAATCAGTTGGAGCTAGCAGCCAGGGGTCCTTTACGACAGACCTTTACCCACAACCCTCTCCT
  ACAACCTGGAAAGGATAACCCTCCCAATATCCCTAGCAGCAGCAGCAAACCAAAGTCAAAGTATCCCTGG
  CACGACACACTTGGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213574 representing NM_005015
  Red=Cloning site Green=Tags(s)
  
  MVTWLYRFLPTSNMAAKLRSLLPPDLRLQFWLHARLQKCFLSRGCGSYCAGAKASPLPGKMAMGLMCGRR
  ELLRLLQSGRRVHSVAGPSQWLGKPLTTRLLFPAAPCCCRPHYLFLAASGPRSLSTSAISFAEVQVQAPP
  VVAATPSPTAVPEVASGETADVVQTAAEQSFAELGLGSYTPVGLIQNLLEFMHVDLGLPWWGAIAACTVF
  ARCLIFPLIVTGQREAARIHNHLPEIQKFSSRIREAKLAGDHIEYYKASSEMALYQKKHGIKLYKPLILP
  VTQAPIFISFFIALREMANLPVPSLQTGGLWWFQDLTVSDPIYILPLAVTATMWAVLELGAETGVQSSDL
  QWMRNVIRMMPLITLPITMHFPTAVFMYWLSSNLFSLVQVSCLRIPAVRTVLKIPQRVVHDLDKLPPREG
  FLESFKKGWKNAEMTRQLREREQRMRNQLELAARGPLRQTFTHNPLLQPGKDNPPNIPSSSSKPKSKYPW
  HDTLG
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005015
• ORF Size: 1485 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005015.3, NP_005006.3
• RefSeq Size: 1737 bp
• RefSeq ORF: 1308 bp
• Locus ID: 5018
• UniProt ID: Q15070
• Cytogenetics: 14q11.2
• Protein Families: Transmembrane
• Protein Pathways: Protein export
• MW: 55.7 kDa
• Gene Summary: This gene encodes an evolutionarily conserved protein that is localized to the inner mitochondrial membrane. The encoded protein is essential for the translocation of the N-terminal tail of subunit 2 of cytochrome c oxidase, and is involved in the assembly of the cytochrome c oxidase and ATPase complexes of the mitochondrial respiratory chain. [provided by RefSeq, Jul 2016]