OXA1L (NM_005015) Human Tagged ORF Clone

CAT#: RG213574

OXA1L (tGFP-tagged) - Human oxidase (cytochrome c) assembly 1-like (OXA1L), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RG213574

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: OXA1L
• Synonyms: OXA1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213574 representing NM_005015
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGATGGGACTAATGTGCGGACGCCGGGAGCTTCTGCGCTTGCTACAGTCCGGGCGTCGGGTCCACA
  GCGTCGCAGGGCCCTCGCAATGGCTTGGGAAACCGCTGACCACACGGCTCCTATTCCCAGTAGCCCCGTG
  CTGCTGTCGCCCACACTACCTCTTCCTTGCGGCTTCCGGCCCCCGCAGCCTCAGTACCTCTGCTATCTCT
  TTTGCAGAAGTCCAGGTTCAGGCCCCTCCTGTTGTTGCTGCAACTCCCTCACCCACAGCAGTACCTGAGG
  TGGCTTCTGGAGAGACTGCAGATGTAGTCCAAACTGCTGCAGAGCAGAGCTTCGCTGAACTGGGGCTGGG
  GTCATACACCCCAGTGGGACTGATCCAGAATTTACTGGAATTTATGCATGTTGATCTGGGCCTACCTTGG
  TGGGGGGCCATTGCTGCATGTACAGTCTTTGCCCGCTGCCTGATTTTTCCTCTCATCGTGACGGGCCAGC
  GAGAGGCAGCCAGGATCCACAATCACTTGCCAGAGATCCAGAAGTTTTCCAGTCGAATCAGAGAGGCCAA
  GTTAGCAGGAGACCATATTGAGTATTACAAGGCTTCCTCGGAGATGGCACTTTACCAGAAAAAACATGGT
  ATTAAACTCTATAAACCTCTCATTCTCCCTGTGACTCAGGCCCCAATCTTCATCTCCTTCTTCATTGCTT
  TGAGAGAGATGGCCAACCTTCCTGTGCCCAGCCTGCAGACAGGTGGCCTCTGGTGGTTCCAGGATCTCAC
  GGTATCCGATCCCATCTACATATTACCACTGGCAGTCACTGCTACAATGTGGGCTGTTCTTGAGCTAGGT
  GCTGAGACAGGTGTGCAAAGTTCTGACCTTCAGTGGATGAGAAATGTCATCAGAATGATGCCCCTGATAA
  CCTTGCCCATAACCATGCATTTCCCCACGGCAGTGTTTATGTACTGGCTCTCCTCCAATTTGTTTTCCCT
  GGTCCAAGTATCCTGTCTCCGGATTCCAGCAGTACGCACTGTACTTAAAATCCCCCAGCGTGTTGTACAT
  GACCTGGACAAATTACCTCCACGGGAAGGCTTCCTAGAGAGCTTCAAAAAAGGCTGGAAAAATGCTGAAA
  TGACGCGTCAGCTGCGAGAGCGTGAACAACGCATGCGGAATCAGTTGGAGCTAGCAGCCAGGGGTCCTTT
  ACGACAGACCTTTACCCACAACCCTCTCCTACAACCTGGAAAGGATAACCCTCCCAATATCCCTAGCAGC
  AGCAGCAAACCAAAGTCAAAGTATCCCTGGCACGACACACTTGGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213574 representing NM_005015
  Red=Cloning site Green=Tags(s)
  
  MAMGLMCGRRELLRLLQSGRRVHSVAGPSQWLGKPLTTRLLFPVAPCCCRPHYLFLAASGPRSLSTSAIS
  FAEVQVQAPPVVAATPSPTAVPEVASGETADVVQTAAEQSFAELGLGSYTPVGLIQNLLEFMHVDLGLPW
  WGAIAACTVFARCLIFPLIVTGQREAARIHNHLPEIQKFSSRIREAKLAGDHIEYYKASSEMALYQKKHG
  IKLYKPLILPVTQAPIFISFFIALREMANLPVPSLQTGGLWWFQDLTVSDPIYILPLAVTATMWAVLELG
  AETGVQSSDLQWMRNVIRMMPLITLPITMHFPTAVFMYWLSSNLFSLVQVSCLRIPAVRTVLKIPQRVVH
  DLDKLPPREGFLESFKKGWKNAEMTRQLREREQRMRNQLELAARGPLRQTFTHNPLLQPGKDNPPNIPSS
  SSKPKSKYPWHDTLG
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005015
• ORF Size: 1485 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005015.5
• RefSeq Size: 1737 bp
• RefSeq ORF: 1308 bp
• Locus ID: 5018
• UniProt ID: Q15070
• Cytogenetics: 14q11.2
• Protein Families: Transmembrane
• Protein Pathways: Protein export
• Gene Summary: This gene encodes an evolutionarily conserved protein that is localized to the inner mitochondrial membrane. The encoded protein is essential for the translocation of the N-terminal tail of subunit 2 of cytochrome c oxidase, and is involved in the assembly of the cytochrome c oxidase and ATPase complexes of the mitochondrial respiratory chain. [provided by RefSeq, Jul 2016]