Collagen III (COL3A1) (NM_000090) Human Tagged Lenti ORF Clone
CAT#: RC211766L1
- LentiORF®
Lenti-ORF clone of COL3A1 (Myc-DDK-tagged)-Human collagen, type III, alpha 1 (COL3A1)
"NM_000090" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Collagen III |
Synonyms | EDS4A; EDSVASC; PMGEDSV |
Vector | pLenti-C-Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC211766).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_000090 |
ORF Size | 4398 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000090.2 |
RefSeq Size | 5489 bp |
RefSeq ORF | 4401 bp |
Locus ID | 1281 |
UniProt ID | P02461 |
Cytogenetics | 2q32.2 |
Domains | COLFI, VWC, Collagen |
Protein Families | Druggable Genome |
Protein Pathways | ECM-receptor interaction, Focal adhesion |
MW | 138.6 kDa |
Gene Summary | This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC211766 | COL3A1 (Myc-DDK-tagged)-Human collagen, type III, alpha 1 (COL3A1) |
USD 1,254.00 |
|
RC211766L2 | Lenti-ORF clone of COL3A1 (mGFP-tagged)-Human collagen, type III, alpha 1 (COL3A1) |
USD 1,554.00 |
|
RC211766L3 | Lenti-ORF clone of COL3A1 (Myc-DDK-tagged)-Human collagen, type III, alpha 1 (COL3A1) |
USD 1,554.00 |
|
RC211766L4 | Lenti-ORF clone of COL3A1 (mGFP-tagged)-Human collagen, type III, alpha 1 (COL3A1) |
USD 1,554.00 |
|
RG211766 | COL3A1 (tGFP-tagged) - Human collagen, type III, alpha 1 (COL3A1) |
USD 1,454.00 |
|
SC308717 | COL3A1 (untagged)-Human collagen, type III, alpha 1 (COL3A1) |
USD 1,882.00 |
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