KAT2B (NM_003884) Human Tagged ORF Clone

CAT#: RC209821

KAT2B (Myc-DDK-tagged)-Human K(lysine) acetyltransferase 2B (KAT2B)

Product Images

Western blot validation of overexpression lysate (Cat# LY418373) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209821 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KAT2B protein (Cat# TP309821). The protein was produced from HEK293T cells transfected with KAT2B cDNA clone (Cat# RC209821) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KAT2B
• Synonyms: CAF; P/CAF; PCAF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209821 representing NM_003884
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGAGGCTGGCGGGGCCGGGCCGGGCGGCTGCGGGGCAGGAGCCGGGGCAGGGGCCGGGCCCGGGG
  CGCTGCCCCCGCAGCCTGCGGCGCTTCCGCCCGCGCCCCCGCAGGGCTCCCCCTGCGCCGCTGCCGCCGG
  GGGCTCGGGCGCCTGCGGTCCGGCGACGGCAGTGGCTGCAGCGGGCACGGCCGAAGGACCGGGAGGCGGT
  GGCTCGGCCCGAATCGCCGTGAAGAAAGCGCAACTACGCTCCGCTCCGCGGGCCAAGAAACTGGAGAAAC
  TCGGAGTGTACTCCGCCTGCAAGGCCGAGGAGTCTTGTAAATGTAATGGCTGGAAAAACCCTAACCCCTC
  ACCCACTCCCCCCAGAGCCGACCTGCAGCAAATAATTGTCAGTCTAACAGAATCCTGTCGGAGTTGTAGC
  CATGCCCTAGCTGCTCATGTTTCCCACCTGGAGAATGTGTCAGAGGAAGAAATGAACAGACTCCTGGGAA
  TAGTATTGGATGTGGAATATCTCTTTACCTGTGTCCACAAGGAAGAAGATGCAGATACCAAACAAGTTTA
  TTTCTATCTATTTAAGCTCTTGAGAAAGTCTATTTTACAAAGAGGAAAACCTGTGGTTGAAGGCTCTTTG
  GAAAAGAAACCCCCATTTGAAAAACCTAGCATTGAACAGGGTGTGAATAACTTTGTGCAGTACAAATTTA
  GTCACCTGCCAGCAAAAGAAAGGCAAACAATAGTTGAGTTGGCAAAAATGTTCCTAAACCGCATCAACTA
  TTGGCATCTGGAGGCACCATCTCAACGAAGACTGCGATCTCCCAATGATGATATTTCTGGATACAAAGAG
  AACTACACAAGGTGGCTGTGTTACTGCAACGTGCCACAGTTCTGCGACAGTCTACCTCGGTACGAAACCA
  CACAGGTGTTTGGGAGAACATTGCTTCGCTCGGTCTTCACTGTTATGAGGCGACAACTCCTGGAACAAGC
  AAGACAGGAAAAAGATAAACTGCCTCTTGAAAAACGAACTCTAATCCTCACTCATTTCCCAAAATTTCTG
  TCCATGCTAGAAGAAGAAGTATATAGTCAAAACTCTCCCATCTGGGATCAGGATTTTCTCTCAGCCTCTT
  CCAGAACCAGCCAGCTAGGCATCCAAACAGTTATCAATCCACCTCCTGTGGCTGGGACAATTTCATACAA
  TTCAACCTCATCTTCCCTTGAGCAGCCAAACGCAGGGAGCAGCAGTCCTGCCTGCAAAGCCTCTTCTGGA
  CTTGAGGCAAACCCAGGAGAAAAGAGGAAAATGACTGATTCTCATGTTCTGGAGGAGGCCAAGAAACCCC
  GAGTTATGGGGGATATTCCGATGGAATTAATCAACGAGGTTATGTCTACCATCACGGACCCTGCAGCAAT
  GCTTGGACCAGAGACCAATTTTCTGTCAGCACACTCGGCCAGGGATGAGGCGGCAAGGTTGGAAGAGCGC
  AGGGGTGTAATTGAATTTCACGTGGTTGGCAATTCCCTCAACCAGAAACCAAACAAGAAGATCCTGATGT
  GGCTGGTTGGCCTACAGAACGTTTTCTCCCACCAGCTGCCCCGAATGCCAAAAGAATACATCACACGGCT
  CGTCTTTGACCCGAAACACAAAACCCTTGCTTTAATTAAAGATGGCCGTGTTATTGGTGGTATCTGTTTC
  CGTATGTTCCCATCTCAAGGATTCACAGAGATTGTCTTCTGTGCTGTAACCTCAAATGAGCAAGTCAAGG
  GCTATGGAACACACCTGATGAATCATTTGAAAGAATATCACATAAAGCATGACATCCTGAACTTCCTCAC
  ATATGCAGATGAATATGCAATTGGATACTTTAAGAAACAGGGTTTCTCCAAAGAAATTAAAATACCTAAA
  ACCAAATATGTTGGCTATATCAAGGATTATGAAGGAGCCACTTTAATGGGATGTGAGCTAAATCCACGGA
  TCCCGTACACAGAATTTTCTGTCATCATTAAAAAGCAGAAGGAGATAATTAAAAAACTGATTGAAAGAAA
  ACAGGCACAAATTCGAAAAGTTTACCCTGGACTTTCATGTTTTAAAGATGGAGTTCGACAGATTCCTATA
  GAAAGCATTCCTGGAATTAGAGAGACAGGCTGGAAACCGAGTGGAAAAGAGAAAAGTAAAGAGCCCAGAG
  ACCCTGACCAGCTTTACAGCACGCTCAAGAGCATCCTCCAGCAGGTGAAGAGCCATCAAAGCGCTTGGCC
  CTTCATGGAACCTGTGAAGAGAACAGAAGCTCCAGGATATTATGAAGTTATAAGGTTCCCCATGGATCTG
  AAAACCATGAGTGAACGCCTCAAGAATAGGTACTACGTGTCTAAGAAATTATTCATGGCAGACTTACAGC
  GAGTCTTTACCAATTGCAAAGAGTACAACCCCCCTGAGAGTGAATACTACAAATGTGCCAATATCCTGGA
  GAAATTCTTCTTCAGTAAAATTAAGGAAGCTGGATTAATTGACAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209821 representing NM_003884
  Red=Cloning site Green=Tags(s)
  
  MSEAGGAGPGGCGAGAGAGAGPGALPPQPAALPPAPPQGSPCAAAAGGSGACGPATAVAAAGTAEGPGGG
  GSARIAVKKAQLRSAPRAKKLEKLGVYSACKAEESCKCNGWKNPNPSPTPPRADLQQIIVSLTESCRSCS
  HALAAHVSHLENVSEEEMNRLLGIVLDVEYLFTCVHKEEDADTKQVYFYLFKLLRKSILQRGKPVVEGSL
  EKKPPFEKPSIEQGVNNFVQYKFSHLPAKERQTIVELAKMFLNRINYWHLEAPSQRRLRSPNDDISGYKE
  NYTRWLCYCNVPQFCDSLPRYETTQVFGRTLLRSVFTVMRRQLLEQARQEKDKLPLEKRTLILTHFPKFL
  SMLEEEVYSQNSPIWDQDFLSASSRTSQLGIQTVINPPPVAGTISYNSTSSSLEQPNAGSSSPACKASSG
  LEANPGEKRKMTDSHVLEEAKKPRVMGDIPMELINEVMSTITDPAAMLGPETNFLSAHSARDEAARLEER
  RGVIEFHVVGNSLNQKPNKKILMWLVGLQNVFSHQLPRMPKEYITRLVFDPKHKTLALIKDGRVIGGICF
  RMFPSQGFTEIVFCAVTSNEQVKGYGTHLMNHLKEYHIKHDILNFLTYADEYAIGYFKKQGFSKEIKIPK
  TKYVGYIKDYEGATLMGCELNPRIPYTEFSVIIKKQKEIIKKLIERKQAQIRKVYPGLSCFKDGVRQIPI
  ESIPGIRETGWKPSGKEKSKEPRDPDQLYSTLKSILQQVKSHQSAWPFMEPVKRTEAPGYYEVIRFPMDL
  KTMSERLKNRYYVSKKLFMADLQRVFTNCKEYNPPESEYYKCANILEKFFFSKIKEAGLIDK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003884
• ORF Size: 2496 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003884.5
• RefSeq Size: 4838 bp
• RefSeq ORF: 2499 bp
• Locus ID: 8850
• UniProt ID: Q92831
• Cytogenetics: 3p24.3
• Domains: Acetyltransf, BROMO
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Notch signaling pathway
• MW: 92.8 kDa
• Gene Summary: CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. [provided by RefSeq, Jul 2008]