Aly (ALYREF) (NM_005782) Human Tagged ORF Clone

USD 98.00

USD 390.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name Aly (ALYREF) (NM_005782) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ALYREF
Synonyms ALY; ALY/REF; BEF; REF; THOC4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC208627 representing NM_005782
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGACAAAATGGACATGTCTCTGGACGACATCATTAAACTGAACCGGAGCCAGCGAGGCGGCCGGG
GCGGGGGCCGGGGCCGCGGCCGGGCCGGCTCCCAGGGCGGCCGCGGCGGTGGGGCGCAGGCCGCCGCGCG
AGTGAATCGAGGCGGCGGGCCCATCCGGAACCGGCCGGCCATCGCCCGCGGCGCGGCCGGCGGAGGCGGC
AGGAACCGACCGGCGCCCTACAGCAGGCCAAAACAACTTCCCGACAAGTGGCAGCACGATCTTTTCGACA
GTGGCTTCGGCGGTGGTGCCGGCGTGGAGACAGGTGGGAAACTGCTGGTGTCCAATCTGGATTTTGGAGT
CTCAGACGCCGATATTCAGGAACTCTTTGCTGAATTTGGAACGCTGAAGAAGGCGGCTGTGCACTATGAT
CGCTCTGGTCGCAGCTTAGGAACAGCAGACGTGCACTTTGAGCGGAAGGCAGATGCCCTGAAGGCCATGA
AGCAGTACAACGGCGTCCCTCTGGATGGCCGCCCCATGAACATTCAGCTTGTCACGTCACAGATTGACGC
ACAGCGGAGGCCTGCACAGAGCGTAAACAGAGGTGGCATGACTAGAAACCGTGGCGCTGGAGGTTTTGGT
GGTGGTGGAGGCACCCGGAGAGGCACCCGCGGAGGCGCCCGTGGAAGAGGCAGAGGTGCCGGCAGGAATT
CAAAGCAGCAGCTTTCGGCAGAGGAGCTGGATGCCCAGCTGGACGCCTATAATGCGAGAATGGACACCAG
T


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208627 representing NM_005782
Red=Cloning site Green=Tags(s)

MADKMDMSLDDIIKLNRSQRGGRGGGRGRGRAGSQGGRGGGAQAAARVNRGGGPIRNRPAIARGAAGGGG
RNRPAPYSRPKQLPDKWQHDLFDSGFGGGAGVETGGKLLVSNLDFGVSDADIQELFAEFGTLKKAAVHYD
RSGRSLGTADVHFERKADALKAMKQYNGVPLDGRPMNIQLVTSQIDAQRRPAQSVNRGGMTRNRGAGGFG
GGGGTRRGTRGGARGRGRGAGRNSKQQLSAEELDAQLDAYNARMDTS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_005782
ORF Size 771 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005782.2, NP_005773.2
RefSeq Size 1113 bp
RefSeq ORF 795 bp
Locus ID 10189
Cytogenetics 17q25.3
Protein Pathways Spliceosome
MW 26.9 kDa
Gene Summary The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. [provided by RefSeq, Jul 2008]

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