Aly (ALYREF) (NM_005782) Human Tagged ORF Clone

CAT#: RG229136

ALYREF (tGFP-tagged) - Human THO complex 4 (THOC4)

Product Images

Circular map for RG229136

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Aly
• Synonyms: ALY; ALY/REF; BEF; REF; THOC4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG229136 representing NM_005782
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGATTCCGCGCCCGCCATGGCCGACAAAATGGACATGTCTCTGGACGACATCATTAAACTGAACC
  GGAGCCAGCGAGGCGGCCGGGGCGGGGGCCGGGGCCGCGGCCGGGCCGGCTCCCAGGGCGGCCGCGGCGG
  TGGGGCGCAGGCCGCCGCGCGAGTGAATCGAGGCGGCGGGCCCATCCGGAACCGGCCGGCCATCGCCCGC
  GGCGCGGCCGGCGGAGGCGGCAGGAACCGACCGGCGCCCTACAGCAGGCCAAAACAACTTCCCGACAAGT
  GGCAGCACGATCTTTTCGACAGTGGCTTCGGCGGTGGTGCCGGCGTGGAGACAGGTGGGAAACTGCTGGT
  GTCCAATCTGGATTTTGGAGTCTCAGACGCCGATATTCAGGAACTCTTTGCTGAATTTGGAACGCTGAAG
  AAGGCGGCTGTGCACTATGATCGCTCTGGTCGCAGCTTAGGAACAGCAGACGTGCACTTTGAGCGGAAGG
  CAGATGCCCTGAAGGCCATGAAGCAGTACAACGGCGTCCCTCTGGATGGCCGCCCCATGAACATTCAGCT
  TGTCACGTCACAGATTGACGCACAGCGGAGGCCTGCACAGAGCGTAAACAGAGGTGGCATGACTAGAAAC
  CGTGGCGCTGGAGGTTTTGGTGGTGGTGGAGGCACCCGGAGAGGCACCCGCGGAGGCGCCCGTGGAAGAG
  GCAGAGGTGCCGGCAGGAATTCAAAGCAGCAGCTTTCGGCAGAGGAGCTGGATGCCCAGCTGGACGCCTA
  TAATGCGAGAATGGACACCAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG229136 representing NM_005782
  Red=Cloning site Green=Tags(s)
  
  MPDSAPAMADKMDMSLDDIIKLNRSQRGGRGGGRGRGRAGSQGGRGGGAQAAARVNRGGGPIRNRPAIAR
  GAAGGGGRNRPAPYSRPKQLPDKWQHDLFDSGFGGGAGVETGGKLLVSNLDFGVSDADIQELFAEFGTLK
  KAAVHYDRSGRSLGTADVHFERKADALKAMKQYNGVPLDGRPMNIQLVTSQIDAQRRPAQSVNRGGMTRN
  RGAGGFGGGGGTRRGTRGGARGRGRGAGRNSKQQLSAEELDAQLDAYNARMDTS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005782
• ORF Size: 792 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005782.4
• RefSeq Size: 1113 bp
• RefSeq ORF: 795 bp
• Locus ID: 10189
• UniProt ID: Q86V81
• Cytogenetics: 17q25.3
• Protein Pathways: Spliceosome
• Gene Summary: The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. [provided by RefSeq, Jul 2008]