SIGLEC8 (NM_014442) Human Tagged ORF Clone

CAT#: RC208582

SIGLEC8 (Myc-DDK-tagged)-Human sialic acid binding Ig-like lectin 8 (SIGLEC8)

Product Images

Western blot validation of overexpression lysate (Cat# LY415267) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208582 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SIGLEC8 protein (Cat# TP308582). The protein was produced from HEK293T cells transfected with SIGLEC8 cDNA clone (Cat# RC208582) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SIGLEC8
• Synonyms: SAF2; SIGLEC-8; SIGLEC8L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208582 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTGCTGCTGCTGCTGCTGCCCCTGCTCTGGGGGACAAAGGGGATGGAGGGAGACAGACAATATG
  GGGATGGTTACTTGCTGCAAGTGCAGGAGCTGGTGACGGTGCAGGAGGGCCTGTGTGTCCATGTGCCCTG
  CTCCTTCTCCTACCCCCAGGATGGCTGGACTGACTCTGACCCAGTTCATGGCTACTGGTTCCGGGCAGGA
  GACAGACCATACCAAGACGCTCCAGTGGCCACAAACAACCCAGACAGAGAAGTGCAGGCAGAGACCCAGG
  GCCGATTCCAACTCCTTGGGGACATTTGGAGCAACGACTGCTCCCTGAGCATCAGAGACGCCAGGAAGAG
  GGATAAGGGGTCATATTTCTTTCGGCTAGAGAGAGGAAGCATGAAATGGAGTTACAAATCACAGTTGAAT
  TACAAAACTAAGCAGCTGTCTGTGTTTGTGACAGCCCTGACCCATAGGCCTGACATCCTCATCCTAGGGA
  CCCTAGAGTCTGGCCACCCCAGGAACCTGACCTGCTCTGTGCCCTGGGCCTGTAAGCAGGGGACACCCCC
  CATGATCTCCTGGATTGGGGCCTCCGTGTCCTCCCCGGGCCCCACTACTGCCCGCTCCTCAGTGCTCACC
  CTTACCCCAAAGCCCCAGGACCACGGCACCAGCCTCACCTGTCAGGTGACCTTGCCTGGGACAGGTGTGA
  CCACGACCAGTACCGTCCGCCTCGATGTGTCCTACCCTCCTTGGAACTTGACCATGACTGTCTTCCAAGG
  AGATGCCACAGCATCCACAGCCCTGGGAAATGGCTCATCTCTTTCAGTCCTTGAGGGCCAGTCTCTGCGC
  CTGGTCTGTGCTGTCAACAGCAATCCCCCTGCCAGGCTGAGCTGGACCCGGGGGAGCCTGACCCTGTGCC
  CCTCACGGTCCTCAAACCCTGGGCTGCTGGAGCTGCCTCGAGTGCACGTGAGGGATGAAGGGGAATTCAC
  CTGCCGAGCTCAGAACGCTCAGGGCTCCCAGCACATTTCCCTGAGCCTCTCCCTGCAGAATGAGGGCACA
  GGCACCTCAAGACCTGTATCACAAGTGACACTGGCAGCAGTCGGGGGAGCTGGAGCCACAGCCCTGGCCT
  TCCTGTCCTTCTGCATCATCTTCATCATAGTGAGGTCCTGCAGGAAGAAATCGGCAAGGCCAGCAGCGGG
  CGTGGGGGATACAGGCATGGAAGATGCAAAGGCCATCAGGGGCTCGGCCTCTCAGGGACCCCTGACTGAA
  TCCTGGAAAGATGGCAACCCCCTGAAGAAGCCTCCCCCAGCTGTTGCCCCCTCGTCAGGGGAGGAAGGAG
  AGCTCCATTATGCAACCCTCAGCTTCCATAAAGTGAAGCCTCAGGACCCGCAGGGACAGGAGGCCACTGA
  CAGTGAATACTCGGAGATCAAGATCCACAAGCGAGAAACTGCAGAGACTCAGGCCTGTTTGAGGAATCAC
  AACCCCTCCAGCAAAGAAGTCAGAGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208582 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLLLLLLLPLLWGTKGMEGDRQYGDGYLLQVQELVTVQEGLCVHVPCSFSYPQDGWTDSDPVHGYWFRAG
  DRPYQDAPVATNNPDREVQAETQGRFQLLGDIWSNDCSLSIRDARKRDKGSYFFRLERGSMKWSYKSQLN
  YKTKQLSVFVTALTHRPDILILGTLESGHPRNLTCSVPWACKQGTPPMISWIGASVSSPGPTTARSSVLT
  LTPKPQDHGTSLTCQVTLPGTGVTTTSTVRLDVSYPPWNLTMTVFQGDATASTALGNGSSLSVLEGQSLR
  LVCAVNSNPPARLSWTRGSLTLCPSRSSNPGLLELPRVHVRDEGEFTCRAQNAQGSQHISLSLSLQNEGT
  GTSRPVSQVTLAAVGGAGATALAFLSFCIIFIIVRSCRKKSARPAAGVGDTGMEDAKAIRGSASQGPLTE
  SWKDGNPLKKPPPAVAPSSGEEGELHYATLSFHKVKPQDPQGQEATDSEYSEIKIHKRETAETQACLRNH
  NPSSKEVRG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014442
• ORF Size: 1497 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014442.2, NP_055257.2
• RefSeq Size: 2967 bp
• RefSeq ORF: 1500 bp
• Locus ID: 27181
• UniProt ID: Q9NYZ4
• Cytogenetics: 19q13.41
• Protein Families: Druggable Genome, Transmembrane
• MW: 54.1 kDa
• Gene Summary: Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May 2010]