SIGLEC8 (NM_014442) Human Untagged Clone

CAT#: SC317839

SIGLEC8 (untagged)-Human sialic acid binding Ig-like lectin 8 (SIGLEC8)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SIGLEC8
• Synonyms: SAF2; SIGLEC-8; SIGLEC8L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC317839 representing NM_014442.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTGCTGCTGCTGCTGCTGCTGCCCCTGCTCTGGGGGACAAAGGGGATGGAGGGAGACAGACAATAT
  GGGGATGGTTACTTGCTGCAAGTGCAGGAGCTGGTGACGGTGCAGGAGGGCCTGTGTGTCCATGTGCCC
  TGCTCCTTCTCCTACCCCCAGGATGGCTGGACTGACTCTGACCCAGTTCATGGCTACTGGTTCCGGGCA
  GGAGACAGACCATACCAAGACGCTCCAGTGGCCACAAACAACCCAGACAGAGAAGTGCAGGCAGAGACC
  CAGGGCCGATTCCAACTCCTTGGGGACATTTGGAGCAACGACTGCTCCCTGAGCATCAGAGACGCCAGG
  AAGAGGGATAAGGGGTCATATTTCTTTCGGCTAGAGAGAGGAAGCATGAAATGGAGTTACAAATCACAG
  TTGAATTACAAAACTAAGCAGCTGTCTGTGTTTGTGACAGCCCTGACCCATAGGCCTGACATCCTCATC
  CTAGGGACCCTAGAGTCTGGCCACTCCAGGAACCTGACCTGCTCTGTGCCCTGGGCCTGTAAGCAGGGG
  ACACCCCCCATGATCTCCTGGATTGGGGCCTCCGTGTCCTCCCCGGGCCCCACTACTGCCCGCTCCTCA
  GTGCTCACCCTTACCCCAAAGCCCCAGGACCACGGCACCAGCCTCACCTGTCAGGTGACCTTGCCTGGG
  ACAGGTGTGACCACGACCAGTACCGTCCGCCTCGATGTGTCCTACCCTCCTTGGAACTTGACCATGACT
  GTCTTCCAAGGAGATGCCACAGCATCCACAGCCCTGGGAAATGGCTCATCTCTTTCAGTCCTTGAGGGC
  CAGTCTCTGCGCCTGGTCTGTGCTGTCAACAGCAATCCCCCTGCCAGGCTGAGCTGGACCCGGGGGAGC
  CTGACCCTGTGCCCCTCACGGTCCTCAAACCCTGGGCTGCTGGAGCTGCCTCGAGTGCACGTGAGGGAT
  GAAGGGGAATTCACCTGCCGAGCTCAGAACGCTCAGGGCTCCCAGCACATTTCCCTGAGCCTCTCCCTG
  CAGAATGAGGGCACAGGCACCTCAAGACCTGTATCACAAGTGACACTGGCAGCAGTCGGGGGAGCTGGA
  GCCACAGCCCTGGCCTTCCTGTCCTTCTGCATCATCTTCATCATAGTGAGGTCCTGCAGGAAGAAATCG
  GCAAGGCCAGCAGCGGGCGTGGGGGATACAGGCATGGAAGATGCAAAGGCCATCAGGGGCTCGGCCTCT
  CAGGGACCCCTGACTGAATCCTGGAAAGATGGCAACCCCCTGAAGAAGCCTCCCCCAGCTGTTGCCCCC
  TCGTCAGGGGAGGAAGGAGAGCTCCATTATGCAACCCTCAGCTTCCATAAAGTGAAGCCTCAGGACCCG
  CAGGGACAGGAGGCCACTGACAGTGAATACTCGGAGATCAAGATCCACAAGCGAGAAACTGCAGAGACT
  CAGGCCTGTTTGAGGAATCACAACCCCTCCAGCAAAGAAGTCAGAGGCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_014442
• Insert Size: 1500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014442.2
• RefSeq Size: 2967 bp
• RefSeq ORF: 1500 bp
• Locus ID: 27181
• UniProt ID: Q9NYZ4
• Cytogenetics: 19q13.41
• Protein Families: Druggable Genome, Transmembrane
• MW: 54 kDa
• Gene Summary: Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May 2010]