BRD9 (NM_001009877) Human Tagged ORF Clone

CAT#: RC208315

BRD9 (Myc-DDK-tagged)-Human bromodomain containing 9 (BRD9), transcript variant 2

Product Images

Circular map for RC208315

• 

  Western blot validation of overexpression lysate (Cat# LY423141) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208315 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BRD9
• Synonyms: LAVS3040; PRO9856
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208315 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGGATACCAAAGTCTTGTATTCAATTTTTTTTTCCTTAAATTGTCAGCCGAAAATGAGAGCACAC
  CTATTCAGCAACTCCTGGAACACTTCCTCCGCCAGCTTCAGAGAAAAGATCCCCATGGATTTTTTGCTTT
  TCCTGTCACGGATGCAATTGCTCCTGGATATTCAATGATAATAAAACATCCCATGGATTTTGGCACCATG
  AAAGACAAAATTGTAGCTAATGAATACAAGTCAGTTACGGAATTTAAGGCAGATTTCAAGCTGATGTGTG
  ATAATGCAATGACATACAATAGGCCAGATACCGTGTACTACAAGTTGGCGAAGAAGATCCTTCACGCAGG
  CTTTAAGATGATGAGCAAACAGGCAGCTCTTTTGGGCAATGAAGATACAGCTGTTGAGGAACCTGTCCCT
  GAAGTTGTACCAGTACAAGTAGAAACTGCCAAGAAATCCAAAAAGCCGAGTAGAGAAGTTATCAGCTGCA
  TGTTTGAGCCTGAAGGGAATGCCTGCAGCTTGACGGACAGTACCGCAGAGGAGCACGTGCTGGCGCTGGT
  GGAGCACGCAGCTGACGAAGCTCGGGACAGGATCAACCGGTTCCTCCCAGGCGGCAAGATGGGCTATCTG
  AAGAGGAACGGGGACGGGAGCCTGCTCTACAGCGTGGTCAACACGGCCGAGCCGGACGCTGATGAGGAGG
  AGACCCACCCGGTGGACTTGAGCTCGCTCTCCAGTAAGCTACTCCCAGGCTTCACCACGCTGGGCTTCAA
  AGACGAGAGAAGAAACAAAGTCACCTTTCTCTCCAGTGCCACTACTGCGCTTTCGATGCAGAATAATTCA
  GTATTTGGCGACTTGAAGTCGGACGAGATGGAGCTGCTCTACTCAGCCTACGGAGATGAGACAGGCGTGC
  AGTGTGCGCTGAGCCTGCAGGAGTTTGTGAAGGATGCTGGGAGCTACAGCAAGAAAGTGGTGGACGACCT
  CCTGGACCAGATCACAGGCGGAGACCACTCTAGGACGCTCTTCCAGCTGAAGCAGAGAAGAAATGTTCCC
  ATGAAGCCTCCAGATGAAGCCAAGGTTGGGGACACCCTAGGAGACAGCAGCAGCTCTGTTCTGGAGTTCA
  TGTCGATGAAGTCCTATCCCGACGTTTCTGTGGATATCTCCATGCTCAGCTCTCTGGGGAAGGTGAAGAA
  GGAGCTGGACCCTGACGACAGCCATTTGAACTTGGATGAGACGACGAAGCTCCTGCAGGACCTGCACGAA
  GCACAGGCGGAGCGCGGCGGCTCTCGGCCGTCGTCCAACCTCAGCTCCCTGTCCAACGCCTCCGAGAGGG
  ACCAGCACCACCTGGGAAGCCCTTCTCGCCTGAGTGTCGGGGAGCAGCCAGACGTCACCCACGACCCCTA
  TGAGTTTCTTCAGTCTCCAGAGCCTGCGGCCTCTGCCAAGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208315 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKGYQSLVFNFFFLKLSAENESTPIQQLLEHFLRQLQRKDPHGFFAFPVTDAIAPGYSMIIKHPMDFGTM
  KDKIVANEYKSVTEFKADFKLMCDNAMTYNRPDTVYYKLAKKILHAGFKMMSKQAALLGNEDTAVEEPVP
  EVVPVQVETAKKSKKPSREVISCMFEPEGNACSLTDSTAEEHVLALVEHAADEARDRINRFLPGGKMGYL
  KRNGDGSLLYSVVNTAEPDADEEETHPVDLSSLSSKLLPGFTTLGFKDERRNKVTFLSSATTALSMQNNS
  VFGDLKSDEMELLYSAYGDETGVQCALSLQEFVKDAGSYSKKVVDDLLDQITGGDHSRTLFQLKQRRNVP
  MKPPDEAKVGDTLGDSSSSVLEFMSMKSYPDVSVDISMLSSLGKVKKELDPDDSHLNLDETTKLLQDLHE
  AQAERGGSRPSSNLSSLSNASERDQHHLGSPSRLSVGEQPDVTHDPYEFLQSPEPAASAKT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009877
• ORF Size: 1443 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009877.1, NP_001009877.1
• RefSeq Size: 2526 bp
• RefSeq ORF: 1635 bp
• Locus ID: 65980
• UniProt ID: Q9H8M2
• Cytogenetics: 5p15.33
• MW: 53.2 kDa
• Gene Summary: Plays a role in chromatin remodeling and regulation of transcription (PubMed:22464331, PubMed:26365797). Acts as a chromatin reader that recognizes and binds acylated histones: binds histones that are acetylated and/or butyrylated (PubMed:26365797). Component of SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (PubMed:29374058).[UniProtKB/Swiss-Prot Function]