BRD9 (NM_001009877) Human Untagged Clone

CAT#: SC319962

BRD9 (untagged)-Human bromodomain containing 9 (BRD9), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BRD9
• Synonyms: LAVS3040; PRO9856
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001009877.1 GGAAGAGAAGAAGCGGAAGCGAGAGAGGGAGCACTGTGACACGGAGGGAGAGGCTGACGA
  CTTTGATCCTGGGAAGAAGGTGGAGGTGGAGCCGCCCCCAGATCGGCCAGTCCGAGCGTG
  CCGGACACAGCCAGTTCTCGGTGGAACTTAAAATGCTGTGAGACACCAGACAGACAGATA
  CTGTGAACTTGGAGCTCTCTAATGAAGGGATACCAAAGTCTTGTATTCAATTTTTTTTTC
  CTTAAATTGTCAGCCGAAAATGAGAGCACACCTATTCAGCAACTCCTGGAACACTTCCTC
  CGCCAGCTTCAGAGAAAAGATCCCCATGGATTTTTTGCTTTTCCTGTCACGGATGCAATT
  GCTCCTGGATATTCAATGATAATAAAACATCCCATGGATTTTGGCACCATGAAAGACAAA
  ATTGTAGCTAATGAATACAAGTCAGTTACGGAATTTAAGGCAGATTTCAAGCTGATGTGT
  GATAATGCAATGACATACAATAGGCCAGATACCGTGTACTACAAGTTGGCGAAGAAGATC
  CTTCACGCAGGCTTTAAGATGATGAGCAAACAGGCAGCTCTTTTGGGCAATGAAGATACA
  GCTGTTGAGGAACCTGTCCCTGAAGTTGTACCAGTACAAGTAGAAACTGCCAAGAAATCC
  AAAAAGCCGAGTAGAGAAGTTATCAGCTGCATGTTTGAGCCTGAAGGGAATGCCTGCAGC
  TTGACGGACAGTACCGCAGAGGAGCACGTGCTGGCGCTGGTGGAGCACGCAGCTGACGAA
  GCTCGGGACAGGATCAACCGGTTCCTCCCAGGCGGCAAGATGGGCTATCTGAAGAGGAAC
  GGGGACGGGAGCCTGCTCTACAGCGTGGTCAACACGGCCGAGCCGGACGCTGATGAGGAG
  GAGACCCACCCGGTGGACTTGAGCTCGCTCTCCAGTAAGCTACTCCCAGGCTTCACCACG
  CTGGGCTTCAAAGACGAGAGAAGAAACAAAGTCACCTTTCTCTCCAGTGCCACTACTGCG
  CTTTCGATGCAGAATAATTCAGTATTTGGCGACTTGAAGTCGGACGAGATGGAGCTGCTC
  TACTCAGCCTACGGAGATGAGACAGGCGTGCAGTGTGCGCTGAGCCTGCAGGAGTTTGTG
  AAGGATGCTGGGAGCTACAGCAAGAAAGTGGTGGACGACCTCCTGGACCAGATCACAGGC
  GGAGACCACTCTAGGACGCTCTTCCAGCTGAAGCAGAGAAGAAATGTTCCCATGAAGCCT
  CCAGATGAAGCCAAGGTTGGGGACACCCTAGGAGACAGCAGCAGCTCTGTTCTGGAGTTC
  ATGTCGATGAAGTCCTATCCCGACGTTTCTGTGGATATCTCCATGCTCAGCTCTCTGGGG
  AAGGTGAAGAAGGAGCTGGACCCTGACGACAGCCATTTGAACTTGGATGAGACGACGAAG
  CTCCTGCAGGACCTGCACGAAGCACAGGCGGAGCGCGGCGGCTCTCGGCCGTCGTCCAAC
  CTCAGCTCCCTGTCCAACGCCTCCGAGAGGGACCAGCACCACCTGGGAAGCCCTTCTCGC
  CTGAGTGTCGGGGAGCAGCCAGACGTCACCCACGACCCCTATGAGTTTCTTCAGTCTCCA
  GAGCCTGCGGCCTCTGCCAAGACCTAACTCTAGACCACCTTCAGCTCTTTTATTTTATTT
  TTTTAGTTTTATTTTGCACGTGTAGAGTTTTTGTCATCAGACAAGGACTTTGATCCTGTC
  CCCTTTGGCATGCGGGAAGCAGCCGCGGGGAGGTAATGAATTGTCTGTGGTATCATGTCA
  GCAGAGTCTCCAAGCCCCACGAACCCTGAGGAGTGGAGTCATACGCGAAGGCCATATGGC
  CATCGTGTCAGCAGAGAGAGTCTCTGTACACAGCCCCGTGAACCCTGAGGAGTGGAGTCA
  TACACGAAGGGCGTGTGGCCATCGTGTCAGCAGAGAGAGTCTCTGTACACAGCCCCGTGA
  ACCCTGAGGAGTGGAGTCATACGCGAAGGGTGTGTGGCCAGGCTGCAGAGCTGCGTGCCG
  TTTGTGTCCGAGCATCACGTGTGGCTCCAGCCCTTGTTTCTGCCAGTGTAGACACCTCTG
  TCTGCCCCACTGTCCTGGGGTCGCTCTTGGGAGGCACAGGCATGGGTGTGTCTGGCCTCA
  TTCTGTATCAGTCCAGTGTGTTCCTGTCATAGTTTGTGTCTCCCAGGCAGGCCATGGTAG
  GGGCCTCGCAGGGGCCATTGGGGAGCACAGGGCCAGGCTGGGGTGAGGAGAGCTCCCCTG
  TTTTCTGTTTAATTGATGAGCCTGGGAAAGGAGTGTGTTCTGCCTGCCCGTTACAGTGGA
  GCGTTCCGTGTCCATAAAACGTTTTCTAACTGGGAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001009877
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009877.1, NP_001009877.1
• RefSeq Size: 2526 bp
• RefSeq ORF: 1446 bp
• Locus ID: 65980
• UniProt ID: Q9H8M2
• Cytogenetics: 5p15.33
• Gene Summary: Plays a role in chromatin remodeling and regulation of transcription (PubMed:22464331, PubMed:26365797). Acts as a chromatin reader that recognizes and binds acylated histones: binds histones that are acetylated and/or butyrylated (PubMed:26365797). Component of SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (PubMed:29374058).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1.