Cullin 3 (CUL3) (NM_003590) Human Tagged ORF Clone

CAT#: RC208066

CUL3 (Myc-DDK-tagged)-Human cullin 3 (CUL3)

Product Images

Circular map for RC208066

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  Western blot validation of overexpression lysate (Cat# LY401191) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208066 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CUL3 protein (Cat# TP308066). The protein was produced from HEK293T cells transfected with CUL3 cDNA clone (Cat# RC208066) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cullin 3
• Synonyms: CUL-3; NEDAUS; PHA2E
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208066 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGAATCTGAGCAAAGGCACGGGCAGCCGGAAGGACACCAAGATGCGGATCCGGGCCTTTCCGATGA
  CCATGGATGAAAAATATGTAAACAGCATTTGGGACCTTCTGAAAAATGCAATTCAAGAAATCCAGCGTAA
  GAATAACAGTGGTCTTAGTTTTGAGGAGCTCTATAGAAATGCATATACAATGGTTTTGCATAAACATGGA
  GAAAAGCTCTACACTGGACTAAGAGAAGTTGTTACCGAACATCTCATAAATAAGGTGCGAGAAGATGTAC
  TAAATTCATTGAATAACAACTTTCTTCAAACGCTAAATCAAGCTTGGAATGATCATCAAACAGCTATGGT
  GATGATTAGAGACATACTAATGTACATGGACCGTGTGTATGTACAACAAAATAATGTGGAGAACGTCTAC
  AATTTGGGATTAATTATTTTTCGAGATCAAGTTGTACGTTATGGGTGTATTAGGGATCATCTACGGCAAA
  CTCTATTGGATATGATTGCAAGAGAGCGGAAAGGAGAAGTCGTAGACAGAGGCGCAATAAGAAATGCTTG
  CCAGATGTTAATGATTTTAGGTCTCGAAGGAAGATCAGTCTATGAAGAAGATTTTGAGGCTCCTTTTTTG
  GAAATGTCTGCAGAATTTTTTCAGATGGAAAGCCAGAAATTTTTAGCAGAAAATAGTGCTTCAGTATATA
  TAAAGAAAGTAGAAGCTAGAATTAATGAAGAAATAGAACGAGTGATGCACTGCCTTGACAAATCAACGGA
  AGAACCAATTGTAAAGGTGGTTGAAAGGGAACTCATTTCCAAGCACATGAAGACTATAGTAGAAATGGAG
  AATTCTGGGCTAGTACATATGTTGAAAAATGGAAAGACAGAAGACCTTGGTTGCATGTACAAGTTATTTA
  GTCGTGTGCCAAATGGTTTGAAAACAATGTGTGAGTGTATGAGTTCCTATTTGAGGGAGCAAGGTAAAGC
  TCTTGTTTCTGAAGAAGGAGAAGGAAAGAATCCTGTTGACTATATCCAGGGCTTATTGGATCTGAAGAGT
  AGGTTCGATCGCTTCCTCCTGGAATCATTCAACAATGACCGTCTCTTTAAACAAACTATTGCGGGTGACT
  TTGAGTATTTTCTCAACCTCAACTCCAGGTCTCCTGAATACCTCTCATTATTTATTGATGATAAGCTGAA
  AAAGGGAGTCAAAGGGCTAACAGAACAAGAAGTAGAAACAATATTGGATAAAGCAATGGTCCTTTTTAGG
  TTTATGCAAGAAAAAGATGTATTTGAACGTTATTATAAACAACACTTGGCAAGGAGACTTCTCACAAATA
  AAAGTGTTTCTGATGACTCTGAAAAAAACATGATATCTAAGTTAAAGACTGAATGTGGATGTCAGTTCAC
  GTCAAAACTGGAAGGAATGTTTAGGGATATGAGCATCTCAAACACAACGATGGATGAATTCAGGCAACAT
  CTACAGGCAACTGGTGTATCTTTAGGTGGTGTTGATCTTACAGTCCGGGTGCTCACGACAGGATATTGGC
  CCACTCAGTCAGCCACACCAAAGTGCAACATCCCACCAGCACCAAGACATGCTTTTGAGATATTCAGAAG
  GTTCTACTTAGCCAAACACAGTGGTCGACAGCTCACACTCCAGCATCATATGGGTTCTGCAGATCTCAAT
  GCCACATTTTATGGACCAGTTAAAAAGGAAGATGGATCTGAAGTTGGTGTTGGAGGTGCACAAGTAACTG
  GCTCTAATACACGGAAGCACATATTGCAAGTTTCCACTTTCCAGATGACCATATTAATGCTCTTTAATAA
  TAGAGAAAAATACACATTTGAGGAAATTCAGCAAGAGACAGATATCCCTGAAAGAGAGCTTGTTAGAGCC
  CTACAGTCCCTCGCCTGTGGTAAACCAACACAGCGGGTTCTTACAAAAGAACCCAAATCAAAGGAAATAG
  AAAATGGTCATATATTTACAGTTAATGATCAGTTCACATCCAAACTACACAGAGTCAAGATTCAAACAGT
  TGCTGCCAAACAAGGTGAATCCGACCCAGAGAGGAAAGAAACAAGGCAGAAAGTAGACGACGACAGAAAA
  CATGAGATAGAAGCTGCTATAGTGCGGATAATGAAATCTAGAAAGAAGATGCAGCACAATGTTCTAGTAG
  CGGAGGTAACTCAGCAGTTGAAGGCGCGATTCTTACCAAGTCCAGTTGTTATTAAGAAACGTATTGAAGG
  ACTTATTGAGAGAGAATATTTGGCACGAACACCTGAGGATCGCAAAGTATACACATATGTAGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208066 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSNLSKGTGSRKDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHG
  EKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVY
  NLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFL
  EMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEME
  NSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKS
  RFDRFLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFR
  FMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQH
  LQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLN
  ATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTFEEIQQETDIPERELVRA
  LQSLACGKPTQRVLTKEPKSKEIENGHIFTVNDQFTSKLHRVKIQTVAAKQGESDPERKETRQKVDDDRK
  HEIEAAIVRIMKSRKKMQHNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPEDRKVYTYVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003590
• ORF Size: 2304 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003590.5
• RefSeq Size: 6790 bp
• RefSeq ORF: 2307 bp
• Locus ID: 8452
• UniProt ID: Q13618
• Cytogenetics: 2q36.2
• Domains: CULLIN
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 88.9 kDa
• Gene Summary: This gene encodes a member of the cullin protein family. The encoded protein plays a critical role in the polyubiquitination and subsequent degradation of specific protein substrates as the core component and scaffold protein of an E3 ubiquitin ligase complex. Complexes including the encoded protein may also play a role in late endosome maturation. Mutations in this gene are a cause of type 2E pseudohypoaldosteronism. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]