XRCC3 (NM_005432) Human Tagged ORF Clone

CAT#: RC200326

XRCC3 (Myc-DDK-tagged)-Human X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3), transcript variant 2

Product Images

Circular map for RC200326

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY XRCC3 (Cat# RC200326, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC3(Cat# TA804755). Positive lysates LY401663 (100ug) and LC401663 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY420224) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214791 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: XRCC3
• Synonyms: CMM6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200326 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTGGATCTACTGGACCTGAATCCCAGAATTATTGCTGCAATTAAGAAAGCCAAACTGAAATCGG
  TAAAGGAGGTTTTACACTTTTCTGGACCAGACTTGAAGAGACTGACCAACCTCTCCAGCCCCGAGGTCTG
  GCACTTGCTGAGAACGGCCTCCTTACACTTGCGGGGAAGCAGCATCCTTACAGCACTGCAGCTGCACCAG
  CAGAAGGAGCGGTTCCCCACGCAGCACCAGCGCCTGAGCCTGGGCTGCCCGGTGCTGGACGCGCTGCTCC
  GCGGTGGCCTGCCCCTGGACGGCATCACTGAGCTGGCCGGACGCAGCTCGGCAGGGAAGACCCAGCTGGC
  GCTGCAGCTCTGCCTGGCTGTGCAGTTCCCGCGGCAGCACGGAGGCCTGGAGGCTGGAGCCGTCTACATC
  TGCACGGAAGACGCCTTCCCGCACAAGCGCCTGCAGCAGCTCATGGCCCAGCAGCCGCGGCTGCGCACTG
  ACGTTCCAGGAGAGCTGCTTCAGAAGCTCCGATTTGGCAGCCAGATCTTCATCGAGCACGTGGCCGATGT
  GGACACCTTGTTGGAGTGTGTGAATAAGAAGGTCCCCGTACTGCTGTCTCGGGGCATGGCTCGCCTGGTG
  GTCATCGACTCGGTGGCAGCCCCATTCCGCTGTGAATTTGACAGCCAGGCCTCCGCCCCCAGGGCCAGGC
  ATCTGCAGTCCCTGGGGGCCACGCTGCGTGAGCTGAGCAGTGCCTTCCAGAGCCCTGTGCTGTGCATCAA
  CCAGGTGACAGAGGCCATGGAGGAGCAGGGCGCAGCACACGGGCCGCTGGGGTTCTGGGACGAACGTGTT
  TCCCCAGCCCTTGGCATAACCTGGGCTAACCAGCTCCTGGTGAGACTGCTGGCTGACCGGCTCCGCGAGG
  AAGAGGCTGCCCTCGGCTGCCCAGCCCGGACCCTGCGGGTGCTCTCTGCCCCCCACCTGCCCCCCTCCTC
  CTGTTCCTACACGATCAGTGCCGAAGGGGTGCGAGGGACACCTGGGACCCAGTCCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200326 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDLDLLDLNPRIIAAIKKAKLKSVKEVLHFSGPDLKRLTNLSSPEVWHLLRTASLHLRGSSILTALQLHQ
  QKERFPTQHQRLSLGCPVLDALLRGGLPLDGITELAGRSSAGKTQLALQLCLAVQFPRQHGGLEAGAVYI
  CTEDAFPHKRLQQLMAQQPRLRTDVPGELLQKLRFGSQIFIEHVADVDTLLECVNKKVPVLLSRGMARLV
  VIDSVAAPFRCEFDSQASAPRARHLQSLGATLRELSSAFQSPVLCINQVTEAMEEQGAAHGPLGFWDERV
  SPALGITWANQLLVRLLADRLREEEAALGCPARTLRVLSAPHLPPSSCSYTISAEGVRGTPGTQSH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005432
• ORF Size: 1038 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005432.4
• RefSeq Size: 2620 bp
• RefSeq ORF: 1041 bp
• Locus ID: 7517
• UniProt ID: O43542
• Cytogenetics: 14q32.33
• Protein Families: Druggable Genome
• Protein Pathways: Homologous recombination
• MW: 37.8 kDa
• Gene Summary: This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]