XRCC3 (NM_005432) Human Untagged Clone

CAT#: SC116733

XRCC3 (untagged)-Human X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: XRCC3
• Synonyms: CMM6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116733 sequence for NM_005432 edited (data generated by NextGen Sequencing)
  ATGGATTTGGATCTACTGGACCTGAATCCCAGAATTATTGCTGCAATTAAGAAAGCCAAA
  CTGAAATCGGTAAAGGAGGTTTTACACTTTTCTGGACCAGACTTGAAGAGACTGACCAAC
  CTCTCCAGCCCCGAGGTCTGGCACTTGCTGAGAACGGCCTCCTTACACTTGCGGGGAAGC
  AGCATCCTTACAGCACTGCAGCTGCACCAGCAGAAGGAGCGGTTCCCCACGCAGCACCAG
  CGCCTGAGCCTGGGCTGCCCGGTGCTGGACGCGCTGCTCCGCGGTGGCCTGCCCCTGGAC
  GGCATCACTGAGCTGGCCGGACGCAGCTCGGCAGGGAAGACCCAGCTGGCGCTGCAGCTC
  TGCCTGGCTGTGCAGTTCCCGCGGCAGCACGGAGGCCTGGAGGCTGGAGCCGTCTACATC
  TGCACGGAAGACGCCTTCCCGCACAAGCGCCTGCAGCAGCTCATGGCCCAGCAGCCGCGG
  CTGCGCACTGACGTTCCAGGAGAGCTGCTTCAGAAGCTCCGATTTGGCAGCCAGATCTTC
  ATCGAGCACGTGGCCGATGTGGACACCTTGTTGGAGTGTGTGAATAAGAAGGTCCCCGTA
  CTGCTGTCTCGGGGCATGGCTCGCCTGGTGGTCATCGACTCGGTGGCAGCCCCATTCCGC
  TGTGAATTTGACAGCCAGGCCTCCGCCCCCAGGGCCAGGCATCTGCAGTCCCTGGGGGCC
  ACGCTGCGTGAGCTGAGCAGTGCCTTCCAGAGCCCTGTGCTGTGCATCAACCAGGTGACA
  GAGGCCATGGAGGAGCAGGGCGCAGCACACGGGCCGCTGGGGTTCTGGGACGAACGTGTT
  TCCCCAGCCCTTGGCATAACCTGGGCTAACCAGCTCCTGGTGAGACTGCTGGCTGACCGG
  CTCCGCGAGGAAGAGGCTGCCCTCGGCTGCCCAGCCCGGACCCTGCGGGTGCTCTCTGCC
  CCCCACCTGCCCCCCTCCTCCTGTTCCTACACGATCAGTGCCGAAGGGGTGCGAGGGACA
  CCTGGGACCCAGTCCCACTGA
  
  Clone variation with respect to NM_005432.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005432 unedited
  GGCTTTCCAGGAATTTGTACTACGATCTCACTATAGGGCGGCCGCGAATTCGCACGAGGG
  GAGACACTTAAGGGAAATTAAACTGCAGAGTGCAAGAGATGCCTCAGTCAAGTCAGCCAA
  AAACACGCGGGTCATCCCCAAGCCCCAGAGAGTGACAGAGCCCCGATGACACGGACACCT
  CGGCTGCTGTCACTTCCCTGGTTCGGGCCTCCCACAGGCTTTGAATTGAAGGCGAGTGCC
  TCAGAATTTGCATCCATTGTTCTGTCTTTCCTGGGAAGTTATTCATCCTGGTGGCCAGCC
  CACCGACAAAATGGATTTGGATCTACTGGACCTGAATCCCAGAATTATTGCTGCAATTAA
  GAAAGCCAAACTGAAATCGGTAAAGGAGGTTTTACACTTTTCTGGACCAGACTTGAAGAG
  ACTGACCAACCTCTCCAGCCCCGAGGTCTGGCACTTGCTGAGAACGGCCTCCTTACACTT
  GCGGGGAAGCAGCATCCTTACAGCACTGCAGCTGCACCAGCAGAAGGAGCGGTTCCCCAC
  GCAGCACCAGCGCCTGAGCCTGNGCTGCCCGGTGCTGGACGCGCTGCTCCGCGGTGGCCT
  GCCCCTGGACGGCATCACTGAGCTGGCCGGACGCAGCTCGGCAGGGAAGACCCAGCTGGC
  GCTGCAGCTCTGCCTGGCTGTGCAGTTCCCGCGGCAGCACGGNAGCCTGGAGGCTGGAGC
  CGTCTACATCTGCACGGAAGACGCCTTCCCGCACAAGCGCCTGCAGCAGCTCATGGCCCA
  GCAGCCGCGGCTGCGCACTGACGTTTCCAGAGAGCTGCTTCAGGAGCTCCGATTTGGCAG
  CCAGATCTTCATCGAGCACGTGGCCGATGTGGACATCCTGCTTGNAGTGTGTGAACAAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005432 unedited
  CCCGGGGCCCCACCACATCTTTTTCTCCNNCCGGNTTTACTTGANACCGCGGCCGCATTC
  TAGATCGAGTTTTTTTTTTTTTTTTTTTTTTGCATCCATTGACATTTATTTGTCAAAGGC
  CTGGTCTGTGCCAGGCAGCATGTGGAGGCACTGAGTGGGGGGTGGAGGGGGTTGTACCGT
  GGGAATCAAGGGGGTCACCACTGTGCCCATGGGGAGGCCACGCCACTCTGCCTGCTGGGT
  GGATATTTCAGCCTGTGCAGCCAGATGTGTTCCCAGGAGGCTCTGCTTTCCAGCCAGATG
  TAAGGCCTGGCCACAGTGTGGGACTGGGTCTGCACAGTGTGGGACTGCAGGACAGGGTCC
  TGCAGGTGGCCCTGCCAGGAGTCCTGCCATATCCCTACCAGAATACCACCCAGCCAGGCC
  AGGGCTGAGCTGGACGCCCAGACCCCCAGGGCCAGGATACACCGGGACCCTCCTTCCTCT
  CAACCTCGGATCCTCTTTGCCCAAAACTGTGTTCCCAGGTGGCTGACGAGCCTGTCACGT
  GCCAACCCTGCGCTGCCTGCGCTCCTCTGACCGGCTGTGGTGCTGGCACAGGCCACACTA
  CTAGAGACAAGGCAAGTCCCCCACCCCCACGCTCTCCTGGACCCTGACACTGGAGGTTGG
  TGGGATATGCTTTTTTCCACTTGTACCACACCTGAAGTTTTGTTGGGCACAGTGTGTGCC
  CAAGTTGGTGCCCCCCGGGAGCCAAGGACCCACAAGTGGGTGAGGAGAAGCCGCTTGCCT
  GAAANCCCATTGTGGCCTGAATGGGGGGGGAATAAAGACTGCCCTTTGTGCACGAAGGGA
  GACCCTCCGGGGCACCGTCCCAGGCTTAGGGGGTTTTAAGCCTCAACCCAGGGGGCAGGC
  CCAAAACCAACCCCTTTTGGCTTTCATTGGAAAAAAAAAAAGGGCGTTTCCAGGGGATCC
  CGTTGGAACCTCCGGCTCCACCCTTGAAAAACCTTTCCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005432
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005432.2, NP_005423.1
• RefSeq Size: 2615 bp
• RefSeq ORF: 1041 bp
• Locus ID: 7517
• UniProt ID: O43542
• Cytogenetics: 14q32.33
• Protein Families: Druggable Genome
• Protein Pathways: Homologous recombination
• Gene Summary: This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks a segment in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein.