DBR1 (NM_016216) Human Tagged ORF Clone

CAT#: RC200024

DBR1 (Myc-DDK-tagged)-Human debranching enzyme homolog 1 (S. cerevisiae) (DBR1)

Product Images

Circular map for RC200024

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  Western blot validation of overexpression lysate (Cat# LY414113) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200024 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DBR1 protein (Cat# TP300024). The protein was produced from HEK293T cells transfected with DBR1 cDNA clone (Cat# RC200024) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DBR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200024 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGTGGCTGTGGCTGGCTGCTGCCACGGCGAGCTGGATAAGATCTATGAGACGCTGGCGCTGGCAG
  AGCGGCGCGGCCCGGGGCCTGTCGACCTCTTGCTGTGCTGCGGCGACTTCCAGGCGGTGCGCAACGAGGC
  GGATCTACGCTGCATGGCCGTGCCGCCCAAGTATCGTCACATGCAAACCTTCTACAGGTATTACTCTGGA
  GAGAAAAAGGCTCCAGTTCTCACGCTCTTCATTGGGGGAAACCATGAAGCCTCAAATCATTTGCAAGAGT
  TACCCTATGGTGGCTGGGTGGCACCAAACATTTATTATTTAGGTTTGGCTGGTGTGGTAAAATACCGAGG
  TGTAAGGATCGGTGGAATCTCTGGTATCTTTAAATCTCATGACTATCGAAAAGGTCATTTTGAGTGCCCC
  CCTTATAATTCATCTACAATCAGGAGTATATATCATGTGAGAAATATTGAAGTCTATAAATTAAAACAGC
  TGAAGCAGCCTATAGATATATTCTTGTCTCATGATTGGCCAAGAAGTATATATCATTATGGAAATAAGAA
  GCAACTTCTTAAGACTAAATCTTTTTTCCGACAAGAAGTGGAAAATAACACATTAGGAAGTCCAGCTGCC
  TCAGAGCTTTTAGAGCATCTCAAACCTACTTATTGGTTTTCTGCCCACCTTCATGTGAAGTTTGCCGCCT
  TGATGCAGCATCAGGCAAAGGATAAAGGACAGACAGCCAGAGCAACCAAATTTTTAGCCTTGGACAAATG
  CTTACCACATAGAGATTTTCTTCAGATATTAGAGATAGAACATGACCCCAGTGCTCCTGATTACTTGGAA
  TATGATATTGAATGGCTCACTATTCTCAGGGCTACGGATGATCTTATTAATGTGACTGGGCGCCTGTGGA
  ATATGCCAGAAAATAATGGCCTGCATGCAAGGTGGGATTATAGTGCAACAGAAGAAGGTATGAAAGAAGT
  ATTGGAAAAATTGAATCATGATCTCAAGGTTCCATGTAACTTTAGTGTAACAGCTGCTTGTTATGATCCT
  AGCAAGCCACAGACACAAATGCAGCTGATTCATAGGATCAATCCTCAGACTACTGAATTTTGTGCCCAAC
  TTGGCATCATAGACATCAATGTTAGGCTTCAGAAGTCCAAGGAAGAACATCATGTGTGTGGTGAATATGA
  AGAACAGGATGATGTGGAGAGTAATGACTCTGGAGAAGACCAGAGTGAATATAATACAGACACATCTGCT
  CTGTCTTCTATTAATCCAGATGAAATAATGTTAGATGAAGAAGAAGATGAAGATAGTATTGTAAGTGCAC
  ATAGTGGCATGAATACACCATCGGTAGAACCTTCTGATCAAGCTTCTGAGTTTTCTGCAAGTTTCTCTGA
  TGTCAGGATCTTGCCAGGCTCTATGATTGTATCTTCTGATGATACGGTGGATTCCACAATTGATAGAGAG
  GGGAAACCTGGTGGGACTGTGGAGTCAGGGAATGGAGAGGACTTAACCAAGGTGCCATTGAAGAGGCTGA
  GTGATGAACATGAACCTGAACAAAGAAAGAAAATTAAGAGGAGGAATCAAGCCATTTACGCTGCAGTGGA
  TGATGATGATGATGATGCAGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200024 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRVAVAGCCHGELDKIYETLALAERRGPGPVDLLLCCGDFQAVRNEADLRCMAVPPKYRHMQTFYRYYSG
  EKKAPVLTLFIGGNHEASNHLQELPYGGWVAPNIYYLGLAGVVKYRGVRIGGISGIFKSHDYRKGHFECP
  PYNSSTIRSIYHVRNIEVYKLKQLKQPIDIFLSHDWPRSIYHYGNKKQLLKTKSFFRQEVENNTLGSPAA
  SELLEHLKPTYWFSAHLHVKFAALMQHQAKDKGQTARATKFLALDKCLPHRDFLQILEIEHDPSAPDYLE
  YDIEWLTILRATDDLINVTGRLWNMPENNGLHARWDYSATEEGMKEVLEKLNHDLKVPCNFSVTAACYDP
  SKPQTQMQLIHRINPQTTEFCAQLGIIDINVRLQKSKEEHHVCGEYEEQDDVESNDSGEDQSEYNTDTSA
  LSSINPDEIMLDEEEDEDSIVSAHSGMNTPSVEPSDQASEFSASFSDVRILPGSMIVSSDDTVDSTIDRE
  GKPGGTVESGNGEDLTKVPLKRLSDEHEPEQRKKIKRRNQAIYAAVDDDDDDAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016216
• ORF Size: 1632 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016216.2, NP_057300.2
• RefSeq Size: 2690 bp
• RefSeq ORF: 1635 bp
• Locus ID: 51163
• UniProt ID: Q9UK59
• Cytogenetics: 3q22.3
• Domains: Metallophos, DBR1
• MW: 61.6 kDa
• Gene Summary: The protein encoded by this gene is an RNA lariat debranching enzyme that hydrolyzes 2'-5' prime branched phosphodiester bonds. The encoded protein specifically targets the bonds at the branch point of excised lariat intron RNA, converting them to linear molecules that are then degraded. This protein may also be involved in retroviral replication. [provided by RefSeq, Nov 2011]