DBR1 (NM_016216) Human Tagged ORF Clone

CAT#: RC200024

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DBR1 (Myc-DDK-tagged)-Human debranching enzyme homolog 1 (S. cerevisiae) (DBR1)



Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 616.00


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Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol DBR1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200024 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGGTGGCTGTGGCTGGCTGCTGCCACGGCGAGCTGGATAAGATCTATGAGACGCTGGCGCTGGCAG
AGCGGCGCGGCCCGGGGCCTGTCGACCTCTTGCTGTGCTGCGGCGACTTCCAGGCGGTGCGCAACGAGGC
GGATCTACGCTGCATGGCCGTGCCGCCCAAGTATCGTCACATGCAAACCTTCTACAGGTATTACTCTGGA
GAGAAAAAGGCTCCAGTTCTCACGCTCTTCATTGGGGGAAACCATGAAGCCTCAAATCATTTGCAAGAGT
TACCCTATGGTGGCTGGGTGGCACCAAACATTTATTATTTAGGTTTGGCTGGTGTGGTAAAATACCGAGG
TGTAAGGATCGGTGGAATCTCTGGTATCTTTAAATCTCATGACTATCGAAAAGGTCATTTTGAGTGCCCC
CCTTATAATTCATCTACAATCAGGAGTATATATCATGTGAGAAATATTGAAGTCTATAAATTAAAACAGC
TGAAGCAGCCTATAGATATATTCTTGTCTCATGATTGGCCAAGAAGTATATATCATTATGGAAATAAGAA
GCAACTTCTTAAGACTAAATCTTTTTTCCGACAAGAAGTGGAAAATAACACATTAGGAAGTCCAGCTGCC
TCAGAGCTTTTAGAGCATCTCAAACCTACTTATTGGTTTTCTGCCCACCTTCATGTGAAGTTTGCCGCCT
TGATGCAGCATCAGGCAAAGGATAAAGGACAGACAGCCAGAGCAACCAAATTTTTAGCCTTGGACAAATG
CTTACCACATAGAGATTTTCTTCAGATATTAGAGATAGAACATGACCCCAGTGCTCCTGATTACTTGGAA
TATGATATTGAATGGCTCACTATTCTCAGGGCTACGGATGATCTTATTAATGTGACTGGGCGCCTGTGGA
ATATGCCAGAAAATAATGGCCTGCATGCAAGGTGGGATTATAGTGCAACAGAAGAAGGTATGAAAGAAGT
ATTGGAAAAATTGAATCATGATCTCAAGGTTCCATGTAACTTTAGTGTAACAGCTGCTTGTTATGATCCT
AGCAAGCCACAGACACAAATGCAGCTGATTCATAGGATCAATCCTCAGACTACTGAATTTTGTGCCCAAC
TTGGCATCATAGACATCAATGTTAGGCTTCAGAAGTCCAAGGAAGAACATCATGTGTGTGGTGAATATGA
AGAACAGGATGATGTGGAGAGTAATGACTCTGGAGAAGACCAGAGTGAATATAATACAGACACATCTGCT
CTGTCTTCTATTAATCCAGATGAAATAATGTTAGATGAAGAAGAAGATGAAGATAGTATTGTAAGTGCAC
ATAGTGGCATGAATACACCATCGGTAGAACCTTCTGATCAAGCTTCTGAGTTTTCTGCAAGTTTCTCTGA
TGTCAGGATCTTGCCAGGCTCTATGATTGTATCTTCTGATGATACGGTGGATTCCACAATTGATAGAGAG
GGGAAACCTGGTGGGACTGTGGAGTCAGGGAATGGAGAGGACTTAACCAAGGTGCCATTGAAGAGGCTGA
GTGATGAACATGAACCTGAACAAAGAAAGAAAATTAAGAGGAGGAATCAAGCCATTTACGCTGCAGTGGA
TGATGATGATGATGATGCAGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200024 protein sequence
Red=Cloning site Green=Tags(s)

MRVAVAGCCHGELDKIYETLALAERRGPGPVDLLLCCGDFQAVRNEADLRCMAVPPKYRHMQTFYRYYSG
EKKAPVLTLFIGGNHEASNHLQELPYGGWVAPNIYYLGLAGVVKYRGVRIGGISGIFKSHDYRKGHFECP
PYNSSTIRSIYHVRNIEVYKLKQLKQPIDIFLSHDWPRSIYHYGNKKQLLKTKSFFRQEVENNTLGSPAA
SELLEHLKPTYWFSAHLHVKFAALMQHQAKDKGQTARATKFLALDKCLPHRDFLQILEIEHDPSAPDYLE
YDIEWLTILRATDDLINVTGRLWNMPENNGLHARWDYSATEEGMKEVLEKLNHDLKVPCNFSVTAACYDP
SKPQTQMQLIHRINPQTTEFCAQLGIIDINVRLQKSKEEHHVCGEYEEQDDVESNDSGEDQSEYNTDTSA
LSSINPDEIMLDEEEDEDSIVSAHSGMNTPSVEPSDQASEFSASFSDVRILPGSMIVSSDDTVDSTIDRE
GKPGGTVESGNGEDLTKVPLKRLSDEHEPEQRKKIKRRNQAIYAAVDDDDDDAA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016216
ORF Size 1632 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016216.2, NP_057300.2
RefSeq Size 2690 bp
RefSeq ORF 1635 bp
Locus ID 51163
UniProt ID Q9UK59
Cytogenetics 3q22.3
Domains Metallophos, DBR1
MW 61.6 kDa
Gene Summary The protein encoded by this gene is an RNA lariat debranching enzyme that hydrolyzes 2'-5' prime branched phosphodiester bonds. The encoded protein specifically targets the bonds at the branch point of excised lariat intron RNA, converting them to linear molecules that are then degraded. This protein may also be involved in retroviral replication. [provided by RefSeq, Nov 2011]
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