DBR1 (NM_016216) Human Untagged Clone

CAT#: SC324429

DBR1 (untagged)-Human debranching enzyme homolog 1 (S. cerevisiae) (DBR1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DBR1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_016216.2 CGGGAGCTGAGCGATCAGTCGGTCCTGGGCTGGCTGTGGTGTAGGGCGTTGCAGGCAGCG
  CTCACCTCCTCAGGCCGGCAGAATGCGGGTGGCTGTGGCTGGCTGCTGCCACGGCGAGCT
  GGATAAGATCTATGAGACGCTGGCGCTGGCAGAGCGGCGCGGCCCGGGGCCTGTCGACCT
  CTTGCTGTGCTGCGGCGACTTCCAGGCGGTGCGCAACGAGGCGGATCTACGCTGCATGGC
  CGTGCCGCCCAAGTATCGTCACATGCAAACCTTCTACAGGTATTACTCTGGAGAGAAAAA
  GGCTCCAGTTCTCACGCTCTTCATTGGGGGAAACCATGAAGCCTCAAATCATTTGCAAGA
  GTTACCCTATGGTGGCTGGGTGGCACCAAACATTTATTATTTAGGTTTGGCTGGTGTGGT
  AAAATACCGAGGTGTAAGGATCGGTGGAATCTCTGGTATCTTTAAATCTCATGACTATCG
  AAAAGGTCATTTTGAGTGCCCCCCTTATAATTCATCTACAATCAGGAGTATATATCATGT
  GAGAAATATTGAAGTCTATAAATTAAAACAGCTGAAGCAGCCTATAGATATATTCTTGTC
  TCATGATTGGCCAAGAAGTATATATCATTATGGAAATAAGAAGCAACTTCTTAAGACTAA
  ATCTTTTTTCCGACAAGAAGTGGAAAATAACACATTAGGAAGTCCAGCTGCCTCAGAGCT
  TTTAGAGCATCTCAAACCTACTTATTGGTTTTCTGCCCACCTTCATGTGAAGTTTGCCGC
  CTTGATGCAGCATCAGGCAAAGGATAAAGGACAGACAGCCAGAGCAACCAAATTTTTAGC
  CTTGGACAAATGCTTACCACATAGAGATTTTCTTCAGATATTAGAGATAGAACATGACCC
  CAGTGCTCCTGATTACTTGGAATATGATATTGAATGGCTCACTATTCTCAGGGCTACGGA
  TGATCTTATTAATGTGACTGGGCGCCTGTGGAATATGCCAGAAAATAATGGCCTGCATGC
  AAGGTGGGATTATAGTGCAACAGAAGAAGGTATGAAAGAAGTATTGGAAAAATTGAATCA
  TGATCTCAAGGTTCCATGTAACTTTAGTGTAACAGCTGCTTGTTATGATCCTAGCAAGCC
  ACAGACACAAATGCAGCTGATTCATAGGATCAATCCTCAGACTACTGAATTTTGTGCCCA
  ACTTGGCATCATAGACATCAATGTTAGGCTTCAGAAGTCCAAGGAAGAACATCATGTGTG
  TGGTGAATATGAAGAACAGGATGATGTGGAGAGTAATGACTCTGGAGAAGACCAGAGTGA
  ATATAATACAGACACATCTGCTCTGTCTTCTATTAATCCAGATGAAATAATGTTAGATGA
  AGAAGAAGATGAAGATAGTATTGTAAGTGCACATAGTGGCATGAATACACCATCGGTAGA
  ACCTTCTGATCAAGCTTCTGAGTTTTCTGCAAGTTTCTCTGATGTCAGGATCTTGCCAGG
  CTCTATGATTGTATCTTCTGATGATACGGTGGATTCCACAATTGATAGAGAGGGGAAACC
  TGGTGGGACTGTGGAGTCAGGGAATGGAGAGGACTTAACCAAGGTGCCATTGAAGAGGCT
  GAGTGATGAACATGAACCTGAACAAAGAAAGAAAATTAAGAGGAGGAATCAAGCCATTTA
  CGCTGCAGTGGATGATGATGATGATGATGCAGCTTAAGATGATTTACTTGTTTTGTTTTT
  GTGTTTGTTTTGCTTTTTTGAGATGGAGTTTCACTCTTGTCACCTAGACTGGAGTGCAAT
  GGCGTGATCTCGGCTCACCGCAACCTCCGCCTCCCAGGTTCAAGCAATTCTCCTGCCTCA
  GCCTCCCAAGTAGGTGGGACTACAGGTGCCCGCCACCACACCCGGCTAATTTTTGCATTT
  TTAGTAATGACAGGGTTTCACCATGTTGGCCAGGCTGGTCTCCAACTCCTGACCTCAGGC
  GATCCACCTGTCTTGGCCTTCTAAAGTGCTGGGATTACAGGCGTGAGCCACTGTGCCTGG
  CCGACAATTTACTTGTCTTAGTATTATATGTAGATATGTGATTTTTTGAGACTGTATTTT
  TAGAGCTGAATTTTTGACTAAAGACTTAACTTTATAATAATGAAGATACATTAGAGAATT
  TTAGTTAGATATTGACTTTATCTTTAGGCTTTTGTATGTTTCAGATGCAAACATATTAAA
  GTTTCATATATTTAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_016216
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016216.2, NP_057300.2
• RefSeq Size: 2667 bp
• RefSeq ORF: 1635 bp
• Locus ID: 51163
• UniProt ID: Q9UK59
• Cytogenetics: 3q22.3
• Domains: Metallophos, DBR1
• Gene Summary: The protein encoded by this gene is an RNA lariat debranching enzyme that hydrolyzes 2'-5' prime branched phosphodiester bonds. The encoded protein specifically targets the bonds at the branch point of excised lariat intron RNA, converting them to linear molecules that are then degraded. This protein may also be involved in retroviral replication. [provided by RefSeq, Nov 2011]