DBR1 (NM_016216) Human Untagged Clone

CAT#: SC114430

DBR1 (untagged)-Human debranching enzyme homolog 1 (S. cerevisiae) (DBR1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DBR1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_016216 edited
  ATTATTTTGCAGGAGTCTACACCCAGGAGGTGGCTATACTTATTAAAGTTGGCGGGAGCT
  GAGCGATCAGTCGGTCCTGGGCTGGCTGTGGTGTAGGGCGTTGCAGGCAGCGCTCACCTC
  CTCAGGCCGGCAGAATGCGGGTGGCTGTGGCTGGCTGCTGCCACGGCGAGCTGGATAAGA
  TCTATGAGACGCTGGCGCTGGCAGAGCGGCGCGGCCCGGGGCCTGTCGACCTCTTGCTGT
  GCTGCGGCGACTTCCAGGCGGTGCGCAACGAGGCGGATCTACGCTGCATGGCCGTGCCGC
  CCAAGTATCGTCACATGCAAACCTTCTACAGGTATTACTCTGGAGAGAAAAAGGCTCCAG
  TTCTCACGCTCTTCATTGGGGGAAACCATGAAGCCTCAAATCATTTGCAAGAGTTACCCT
  ATGGTGGCTGGGTGGCACCAAACATTTATTATTTAGGTTTGGCTGGTGTGGTAAAATACC
  GAGGTGTAAGGATCGGTGGAATCTCTGGTATCTTTAAATCTCATGACTATCGAAAAGGTC
  ATTTTGAGTGCCCCCCTTATAATTCATCTACAATCAGGAGTATATATCATGTGAGAAATA
  TTGAAGTCTATAAATTAAAACAGCTGAAGCAGCCTATAGATATATTCTTGTCTCATGATT
  GGCCAAGAAGTATATATCATTATGGAAATAAGAAGCAACTTCTTAAGACTAAATCTTTTT
  TCCGACAAGAAGTGGAAAATAACACATTAGGAAGTCCAGCTGCCTCAGAGCTTTTAGAGC
  ATCTCAAACCTACTTATTGGTTTTCTGCCCACCTTCATGTGAAGTTTGCCGCCTTGATGC
  AGCATCAGGCAAAGGATAAAGGACAGACAGCCAGAGCAACCAAATTTTTAGCCTTGGACA
  AATGCTTACCACATAGAGATTTTCTTCAGATATTAGAGATAGAACATGACCCCAGTGCTC
  CTGATTACTTGGAATATGATATTGAATGGCTCACTATTCTCAGGGCTACGGATGATCTTA
  TTAATGTGACTGGGCGCCTGTGGAATATGCCAGAAAATAATGGCCTGCATGCAAGGTGGG
  ATTATAGTGCAACAGAAGAAGGTATGAAAGAAGTATTGGAAAAATTGAATCATGATCTCA
  AGGTTCCATGTAACTTTAGTGTAACAGCTGCTTGTTATGATCCTAGCAAGCCACAGACAC
  AAATGCAGCTGATTCATAGGATCAATCCTCAGACTACTGAATTTTGTGCCCAACTTGGCA
  TCATAGACATCAATGTTAGGCTTCAGAAGTCCAAGGAAGAACATCATGTGTGTGGTGAAT
  ATGAAGAACAGGATGATGTGGAGAGTAATGACTCTGGAGAAGACCAGAGTGAATATAATA
  CAGACACATCTGCTCTGTCTTCTATTAATCCAGATGAAATAATGTTAGATGAAGAAGAAG
  ATGAAGATAGTATTGTAAGTGCACATAGTGGCATGAATACACCATCGGTAGAACCTTCTG
  ATCAAGCTTCTGAGTTTTCTGCAAGTTTCTCTGATGTCAGGATCTTGCCAGGCTCTATGA
  TTGTATCTTCTGATGATACGGTGGATTCCACAATTGATAGAGAGGGGAAACCTGGTGGGA
  CTGTGGAGTCAGGGAATGGAGAGGACTTAACCAAGGTGCCATTGAAGAGGCTGAGTGATG
  AACATGAACCTGAACAAAGAAAGAAAATTAAGAGGAGGAATCAAGCCATTTACGCTGCAG
  TGGATGATGATGATGATGATGCAGCTTAAGATGATTTACTTGTTTTGTTTTTGTGTTTGT
  TTTGCTTTTTTGAGATGGAGTTTCACTCTTGTCACCTAGACTGGAGTGCAATGGCGTGAT
  CTCGGCTCACCGCAACCTCCGCCTCCCAGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCA
  AGTAGGTGGGACTACAGGTGCCCGCCACCACACCCGGCTAATTTTTGCATTTTTAGTAAT
  GACAGGGTTTCACCATGTTGGCCAGGCTGGTCTCCAACTCCTGACCTCAGGCGATCCACC
  TGTCTTGGCCTTCTAAAGTGCTGGGATTACAGGCGTGAGCCACTGTGCCTGGCCGACAAT
  TTACTTGTCTTAGTATTATATGTAGATATGTGATTTTTTGAGACTGTATTTTTAGAGCTG
  AATTTTTGACTAAAGACTTAACTTTATAATAATGAAGATACATTAGAGAATTTTAGTTAG
  ATATTGACTTTATCTTCAGGCTTTTGTATGTTTCAGATGCAAACATATTAAAGTTTCATA
  TATTTACTTGACTGGGTATCTTTTTTTTTTGAAGACATACATTATTCACTTCCATTTTTA
  CAATAAACACTTACTTAACATCTACTCTGTGCCAAGCACTCTGCTATAATATGGGACCAT
  AAAGATGAGTAAGACAAGGATTTTTGCCTCCAAGGACTTGTTATCACTGTAAACATTTTG
  ATGTATACAATATGGCTATATTATATTTTGTTTTATAAAATCAAGTCATTGTATATATTA
  TGATGTATGCTTTCTTTTCATTTATTATAAATATTTTTCCTGATCAGCTTTTAATATCTG
  TCAAATAAAAATTGAAATTGAAGATGGTATATAAGATTTCAGGAAATAAACTACTCTGAA
  TACTAAAAAAAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016216
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_016216.2.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016216.2, NP_057300.2
• RefSeq Size: 2667 bp
• RefSeq ORF: 1635 bp
• Locus ID: 51163
• UniProt ID: Q9UK59
• Cytogenetics: 3q22.3
• Domains: Metallophos, DBR1
• Gene Summary: The protein encoded by this gene is an RNA lariat debranching enzyme that hydrolyzes 2'-5' prime branched phosphodiester bonds. The encoded protein specifically targets the bonds at the branch point of excised lariat intron RNA, converting them to linear molecules that are then degraded. This protein may also be involved in retroviral replication. [provided by RefSeq, Nov 2011]