Lima1 (NM_023063) Mouse Tagged ORF Clone

CAT#: MR221683

Lima1 (Myc-DDK-tagged) - Mouse LIM domain and actin binding 1 (Lima1), transcript variant b

Product Images

Circular map for MR221683

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lima1
• Synonyms: Eplin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221683 representing NM_023063
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAACTGTCTGGGAGATTCCAGGCATGAAGCAGAGAAGCCAGAGACGAGCGAAAACACAGAAACTT
  CAGGCAAAATAGAGAAATACAACGTTCCACTGAATAGACTGAAGATGATGTTTGAGAAAGGTGAACACAA
  CCAAACCAAGAGTCTCTGGACCCAAAGCCGAAATGCGGGTGGAAGGAGGCTCTCTGAAAACAACTGTTCC
  CTGGATGACTGGGAAATAGGTGCGGGTCATTTGTCTTCCTCTGCATTCAACTCGGAGAAAAACGAGAGTA
  AGAGGAATCTGGAGCTGCCACGCCTCTCAGAAACCTCCATAAAGGACCGCATGGCCAAGTACCAGGCTGC
  AGTGTCCAAGCAGAGCAGCCCAGCCAGCTACACAAATGAGCTGAAAACCAGTGAAAGCAAAACTCATAAA
  TGGGAACAGAAGGAGAATGTGCCCCCAGGTCCCGAGGCCTGCAGCGTCCATCAGGAAGGAAGCAAGGTTT
  CTACAACTGAGAATAGTCTAGTGGCCCTCTCTGTCCCTGCTGAAGATGACACCTGTAACTCCCAGGTGAA
  GAGCGAGGCCCAGCAGCCTATGCACCCTAAGCCGCTGAGTCCTGACGCCAGGACCTCCAGTCTTCCGGAA
  AGTTCTCCTTCCAAAACAGCGAAGAAGTTTCAGGCGCCGGCAAAGGAGAGCTGCGTGGAGTGTCAGAAGA
  CGGTGTACCCCATGGAACGGCTCCTGGCCAACCAGCAGGTGTTTCACATCAGCTGTTTCCGATGCTCCTA
  CTGCAACAACAAGCTCAGTCTAGGAACATATGCATCCTTACATGGACGAATCTACTGTAAGCCTCACTTC
  AATCAACTCTTCAAGTCCAAAGGCAACTATGACGAGGGCTTTGGGCACAAGCAGCATAAGGATCTGTGGG
  CAAGCAAGAGCGACAATGAGGAGACTTTGGGGAGACCAGCCCAGCCGCCTAATGCAGGGGAGAGCCCCCA
  TAGCCCGGGTGTAGAAGATGCCCCCATCGCCAAGGTCGGCGTGCTGGCGGCAAGTATGGAAGCCAAGGCC
  TCTTCTCAGAGGGAGAGGGAGGATAAGCCTGCGGAGACCAAGAAGCTGAGGATCGCCTGGCCTCCGCCAG
  CCGAGCTGGGCGGTTCCGGAAGTGCCCTGGAGGAAGGGATCAAAGTATCGAAGCCCAAGTGGCCTCCGGA
  GGATGACGTCTGCAAGACGGAGGCCCCGGAGGATGTAGATCTGGACCTGAAGAAGCTGCGGCGGTCTTCG
  TCCCTGAAGGAGAGGAGCCGCCCGTTTACGGTAGCAGCTTCCTTTCGAACCTCTTCCATCAAGAGCCCCA
  AGGCCTCTTCGCCATCTCTCAGGAAAGGTTGGAGCGAGTCCGAGCAGAGTGAGGAGTTTGGAGGAGGGAT
  AGCGACGATGGAAAGGAAACAAACGGAAAATGCCAGACCCTCTGGGGAGAAAGAGAATGTGGGAAAATCA
  CGCTGGCAGGGCGAGGAAGTGCCGAGGAGCAAGGACCGTAGTTCTTTTGAGCTGGAGAGTGAGAATTTTA
  TGGAAAATGGAGCAAACATAGCTGAAGATGACAACCATGTCCACGCACAGCAGTCCCCGTTAGAACCCGA
  GGCTCCGGGTTGGTCTGGCTTTGTAGACACCACGGCTGCTAAAGAATTCACTACCCAGAATCAAAAATCC
  CAAGATGTCGGGTTCTGGGAGGGAGAAGTGGTCCGAGAGCTGTCTGTGGAGGAACAGATAAAGAGAAACC
  GGTACTATGATGAGGACGAGGATGAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221683 representing NM_023063
  Red=Cloning site Green=Tags(s)
  
  MENCLGDSRHEAEKPETSENTETSGKIEKYNVPLNRLKMMFEKGEHNQTKSLWTQSRNAGGRRLSENNCS
  LDDWEIGAGHLSSSAFNSEKNESKRNLELPRLSETSIKDRMAKYQAAVSKQSSPASYTNELKTSESKTHK
  WEQKENVPPGPEACSVHQEGSKVSTTENSLVALSVPAEDDTCNSQVKSEAQQPMHPKPLSPDARTSSLPE
  SSPSKTAKKFQAPAKESCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHF
  NQLFKSKGNYDEGFGHKQHKDLWASKSDNEETLGRPAQPPNAGESPHSPGVEDAPIAKVGVLAASMEAKA
  SSQREREDKPAETKKLRIAWPPPAELGGSGSALEEGIKVSKPKWPPEDDVCKTEAPEDVDLDLKKLRRSS
  SLKERSRPFTVAASFRTSSIKSPKASSPSLRKGWSESEQSEEFGGGIATMERKQTENARPSGEKENVGKS
  RWQGEEVPRSKDRSSFELESENFMENGANIAEDDNHVHAQQSPLEPEAPGWSGFVDTTAAKEFTTQNQKS
  QDVGFWEGEVVRELSVEEQIKRNRYYDEDEDEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023063
• ORF Size: 1779 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023063.4, NP_075550.2
• RefSeq Size: 4110 bp
• RefSeq ORF: 1782 bp
• Locus ID: 65970
• Cytogenetics: 15 56.13 cM
• MW: 66.5 kDa
• Gene Summary: Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments (By similarity). Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake (PubMed:29880681).[UniProtKB/Swiss-Prot Function]