Lima1 (NM_023063) Mouse Untagged Clone

CAT#: MC219412

Lima1 (untagged) - Mouse LIM domain and actin binding 1 (Lima1), transcript variant b, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lima1
• Synonyms: Eplin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219412 representing NM_023063
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAACTGTCTGGGAGATTCCAGGCATGAAGCAGAGAAGCCAGAGACGAGCGAAAACACAGAAACTT
  CAGGCAAAATAGAGAAATACAACGTTCCACTGAATAGACTGAAGATGATGTTTGAGAAAGGTGAACACAA
  CCAAACCAAGAGTCTCTGGACCCAAAGCCGAAATGCGGGTGGAAGGAGGCTCTCTGAAAACAACTGTTCC
  CTGGATGACTGGGAAATAGGTGCGGGTCATTTGTCTTCCTCTGCATTCAACTCGGAGAAAAACGAGAGTA
  AGAGGAATCTGGAGCTGCCACGCCTCTCAGAAACCTCCATAAAGGACCGCATGGCCAAGTACCAGGCTGC
  AGTGTCCAAGCAGAGCAGCCCAGCCAGCTACACAAATGAGCTGAAAACCAGTGAAAGCAAAACTCATAAA
  TGGGAACAGAAGGAGAATGTGCCCCCAGGTCCCGAGGCCTGCAGCGTCCATCAGGAAGGAAGCAAGGTTT
  CTACAACTGAGAATAGTCTAGTGGCCCTCTCTGTCCCTGCTGAAGATGACACCTGTAACTCCCAGGTGAA
  GAGCGAGGCCCAGCAGCCTATGCACCCTAAGCCGCTGAGTCCTGACGCCAGGACCTCCAGTCTTCCGGAA
  AGTTCTCCTTCCAAAACAGCGAAGAAGTTTCAGGCGCCGGCAAAGGAGAGCTGCGTGGAGTGTCAGAAGA
  CGGTGTACCCCATGGAACGGCTCCTGGCCAACCAGCAGGTGTTTCACATCAGCTGTTTCCGATGCTCCTA
  CTGCAACAACAAGCTCAGTCTAGGAACATATGCATCCTTACATGGACGAATCTACTGTAAGCCTCACTTC
  AATCAACTCTTCAAGTCCAAAGGCAACTATGACGAGGGCTTTGGGCACAAGCAGCATAAGGATCTGTGGG
  CAAGCAAGAGCGACAATGAGGAGACTTTGGGGAGACCAGCCCAGCCGCCTAATGCAGGGGAGAGCCCCCA
  TAGCCCGGGTGTAGAAGATGCCCCCATCGCCAAGGTCGGCGTGCTGGCGGCAAGTATGGAAGCCAAGGCC
  TCTTCTCAGAGGGAGAGGGAGGATAAGCCTGCGGAGACCAAGAAGCTGAGGATCGCCTGGCCTCCGCCAG
  CCGAGCTGGGCGGTTCCGGAAGTGCCCTGGAGGAAGGGATCAAAGTATCGAAGCCCAAGTGGCCTCCGGA
  GGATGACGTCTGCAAGACGGAGGCCCCGGAGGATGTAGATCTGGACCTGAAGAAGCTGCGGCGGTCTTCG
  TCCCTGAAGGAGAGGAGCCGCCCGTTTACGGTAGCAGCTTCCTTTCGAACCTCTTCCATCAAGAGCCCCA
  AGGCCTCTTCGCCATCTCTCAGGAAAGGTTGGAGCGAGTCCGAGCAGAGTGAGGAGTTTGGAGGAGGGAT
  AGCGACGATGGAAAGGAAACAAACGGAAAATGCCAGACCCTCTGGGGAGAAAGAGAATGTGGGAAAATCA
  CGCTGGCAGGGCGAGGAAGTGCCGAGGAGCAAGGACCGTAGTTCTTTTGAGCTGGAGAGTGAGAATTTTA
  TGGAAAATGGAGCAAACATAGCTGAAGATGACAACCATGTCCACGCACAGCAGTCCCCGTTAGAACCCGA
  GGCTCCGGGTTGGTCTGGCTTTGTAGACACCACGGCTGCTAAAGAATTCACTACCCAGAATCAAAAATCC
  CAAGATGTCGGGTTCTGGGAGGGAGAAGTGGTCCGAGAGCTGTCTGTGGAGGAACAGATAAAGAGAAACC
  GGTACTATGATGAGGACGAGGATGAAGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023063
• Insert Size: 1782 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023063.4, NP_075550.2
• RefSeq Size: 4117 bp
• RefSeq ORF: 1782 bp
• Locus ID: 65970
• Cytogenetics: 15 56.13 cM
• Gene Summary: Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments (By similarity). Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake (PubMed:29880681).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (b) uses an alternate 5'-terminal exon, lacks part of the 5' coding region, and initiates translation from a downstream start codon, compared to variant a. The encoded isoform (b) has a shorter N-terminus, compared to isoform a.