Neurl1a (NM_001163480) Mouse Tagged ORF Clone

CAT#: MR217666

Neurl1a (Myc-DDK-tagged) - Mouse neuralized homolog 1A (Drosophila) (Neurl1a), transcript variant 2

Product Images

Circular map for MR217666

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Neurl1a
• Synonyms: 2410129E16Rik; AI450910; AI481072; Neu1; Neur1; Neurl; Neurl1; Nlz; Rnf67
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217666 representing NM_001163480
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGGACAGATCACAAGGAACACTATCCACGACTCCATCGGGGGCTCCTTCCCGGTGCCCTCTCACC
  GATGCCATCACAAGCAGAAGCATTGCCCGCCTACGCTGTCAGGTGGGGGGCTCCCGGCCACGCCGCTGCT
  CTTCCATCCCCACACTAAGGGCTCCCAGATCCTCATGGACCTCAGCCACAAGGCCGTCAAGAGGCAGGCC
  AGCTTTTGCAATGCCATCACCTTCAGTAACCGCCCGGTGCTCATCTACGAGCAAGTCAGGCTGAAGATCA
  CCAAGAAGCAATGCTGCTGGAGCGGGGCCCTGCGACTTGGCTTCACCAGCAAGGACCCTTCCCGCATCCA
  CCCCGACTCGCTGCCCAAGTACGCCTGCCCTGACCTGGTGTCTCAGAGTGGCTTCTGGGCCAAAGCATTG
  CCTGAGGAGTTTGCCAACGAGGGCAACATCATTGCCTTCTGGGTGGACAAGAAGGGCCGCGTCTTCTACC
  GGATCAATGAGTCAGCTGCTATGCTTTTCTTCAGTGGGGTCCGGACGGTGGACCCGCTCTGGGCCCTGGT
  GGACGTCTACGGCCTCACGCGGGGTGTCCAGCTGCTAGACAGCGAGCTGGTGCTGCCCGACTGCCTGCGG
  CCGCGCTCCTTCACCGCGCTGCGGCGGCCGTCGCTGCGGTGCGAGGCGGATGAGGCGCGCCTGTCGGTGA
  GCCTGTGCGACCTCAACGTGCCGGGAGCCGACGGCGACGACGGCGCACCGCCTGCCGGCTGCCCGATCCC
  GCAGAACTCGCTCAATTCTCAGCACAGCCGCGCGCTGCCGGCGCAGCTCGACGGCGACCTGCGCTTCCAC
  GCGCTTCGCGCCGGCGCGCACGTCCGCATCCTGGACGAGCAGACGGTGGCGCGCCTGGAGCACGGGCGCG
  ACGAGCGCGCGCTCGTCTTCACCAGCCGGCCTGTGCGCGTGGCCGAGACCATCTTCATCAAGGTCACGCG
  CTCGGGCGGGGGGCGAGCGGGCGCGCTGTCCTTCGGGGTCACCACGTGTGACCCTGGCACGCTGCGGCCC
  GCCGACCTGCCCTTCAGCCCCGAGGCCCTGGTGGACCGCAAGGAGTTCTGGGCGGTGTGTCGCGTGCCCG
  GGCCTCTGCACAGCGGCGACATCCTGGGCCTGGTGGTCAACGCGGACGGAGAGCTGCACCTGAGTCACAA
  CGGCGCGGCGGCCGGCATGCAGCTGTGCGTGGATGCCTCGCAGCCCCTCTGGATGCTCTTCAGCCTGCAT
  GGCGCCATCACGCAGGTCCGCATCCTCGGCTCCACCATCATGACTGAACGGGGTGGCCCATCTCTCCCCT
  GCTCACCTGCCTCCACTCCAACCTCACCCAGTGCCCTGGGCATCCGCCTCTCTGACCCCCTGCTCAGCAC
  CTGCGGTTCTGGGCCCCTAGGTGGCTCTGCTGGAGGGACAGCCCCCAACTCACCTGTGAGCCTGCCCGAG
  TCACCGGTGACCCCAGGTCTGGGCCAGTGGAGTGATGAATGCACCATTTGCTATGAACACGCAGTGGACA
  CAGTCATCTACACGTGTGGCCACATGTGCCTGTGCTACTCCTGTGGCCTGCGCCTCAAGAAGGCCCTGCA
  CGCCTGCTGCCCCATCTGCCGTCGCCCCATCAAGGACATCATCAAGACCTACCGCAGCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217666 representing NM_001163480
  Red=Cloning site Green=Tags(s)
  
  MGGQITRNTIHDSIGGSFPVPSHRCHHKQKHCPPTLSGGGLPATPLLFHPHTKGSQILMDLSHKAVKRQA
  SFCNAITFSNRPVLIYEQVRLKITKKQCCWSGALRLGFTSKDPSRIHPDSLPKYACPDLVSQSGFWAKAL
  PEEFANEGNIIAFWVDKKGRVFYRINESAAMLFFSGVRTVDPLWALVDVYGLTRGVQLLDSELVLPDCLR
  PRSFTALRRPSLRCEADEARLSVSLCDLNVPGADGDDGAPPAGCPIPQNSLNSQHSRALPAQLDGDLRFH
  ALRAGAHVRILDEQTVARLEHGRDERALVFTSRPVRVAETIFIKVTRSGGGRAGALSFGVTTCDPGTLRP
  ADLPFSPEALVDRKEFWAVCRVPGPLHSGDILGLVVNADGELHLSHNGAAAGMQLCVDASQPLWMLFSLH
  GAITQVRILGSTIMTERGGPSLPCSPASTPTSPSALGIRLSDPLLSTCGSGPLGGSAGGTAPNSPVSLPE
  SPVTPGLGQWSDECTICYEHAVDTVIYTCGHMCLCYSCGLRLKKALHACCPICRRPIKDIIKTYRSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163480
• ORF Size: 1671 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163480.1, NP_001156952.1
• RefSeq Size: 3690 bp
• RefSeq ORF: 1674 bp
• Locus ID: 18011
• UniProt ID: Q923S6
• Cytogenetics: 19 39.39 cM
• MW: 60.4 kDa
• Gene Summary: Plays a role in hippocampal-dependent synaptic plasticity, learning and memory. Involved in the formation of spines and functional synaptic contacts by modulating the translational activity of the cytoplasmic polyadenylation element-binding protein CPEB3. Promotes ubiquitination of CPEB3, and hence induces CPEB3-dependent mRNA translation activation of glutamate receptor GRIA1 and GRIA2. Can function as an E3 ubiquitin-protein ligase to activate monoubiquitination of JAG1 (in vitro), thereby regulating the Notch pathway. Acts as a tumor suppressor; inhibits malignant cell transformation of medulloblastoma (MB) cells by inhibiting the Notch signaling pathway.[UniProtKB/Swiss-Prot Function]