Neurl1a (NM_001163480) Mouse Untagged Clone

CAT#: MC218950

Neurl1a (untagged) - Mouse neuralized homolog 1A (Drosophila) (Neurl1a), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Neurl1a
• Synonyms: 2410129E16Rik; AI450910; AI481072; Neu1; Neur1; Neurl; Neurl1; Nlz; Rnf67
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218950 representing NM_001163480
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGGACAGATCACAAGGAACACTATCCACGACTCCATCGGGGGCTCCTTCCCGGTGCCCTCTCACC
  GATGCCATCACAAGCAGAAGCATTGCCCGCCTACGCTGTCAGGTGGGGGGCTCCCGGCCACGCCGCTGCT
  CTTCCATCCCCACACTAAGGGCTCCCAGATCCTCATGGACCTCAGCCACAAGGCCGTCAAGAGGCAGGCC
  AGCTTTTGCAATGCCATCACCTTCAGTAACCGCCCGGTGCTCATCTACGAGCAAGTCAGGCTGAAGATCA
  CCAAGAAGCAATGCTGCTGGAGCGGGGCCCTGCGACTTGGCTTCACCAGCAAGGACCCTTCCCGCATCCA
  CCCCGACTCGCTGCCCAAGTACGCCTGCCCTGACCTGGTGTCTCAGAGTGGCTTCTGGGCCAAAGCATTG
  CCTGAGGAGTTTGCCAACGAGGGCAACATCATTGCCTTCTGGGTGGACAAGAAGGGCCGCGTCTTCTACC
  GGATCAATGAGTCAGCTGCTATGCTTTTCTTCAGTGGGGTCCGGACGGTGGACCCGCTCTGGGCCCTGGT
  GGACGTCTACGGCCTCACGCGGGGTGTCCAGCTGCTAGACAGCGAGCTGGTGCTGCCCGACTGCCTGCGG
  CCGCGCTCCTTCACCGCGCTGCGGCGGCCGTCGCTGCGGTGCGAGGCGGATGAGGCGCGCCTGTCGGTGA
  GCCTGTGCGACCTCAACGTGCCGGGAGCCGACGGCGACGACGGCGCACCGCCTGCCGGCTGCCCGATCCC
  GCAGAACTCGCTCAATTCTCAGCACAGCCGCGCGCTGCCGGCGCAGCTCGACGGCGACCTGCGCTTCCAC
  GCGCTTCGCGCCGGCGCGCACGTCCGCATCCTGGACGAGCAGACGGTGGCGCGCCTGGAGCACGGGCGCG
  ACGAGCGCGCGCTCGTCTTCACCAGCCGGCCTGTGCGCGTGGCCGAGACCATCTTCATCAAGGTCACGCG
  CTCGGGCGGGGGGCGAGCGGGCGCGCTGTCCTTCGGGGTCACCACGTGTGACCCTGGCACGCTGCGGCCC
  GCCGACCTGCCCTTCAGCCCCGAGGCCCTGGTGGACCGCAAGGAGTTCTGGGCGGTGTGTCGCGTGCCCG
  GGCCTCTGCACAGCGGCGACATCCTGGGCCTGGTGGTCAACGCGGACGGAGAGCTGCACCTGAGTCACAA
  CGGCGCGGCGGCCGGCATGCAGCTGTGCGTGGATGCCTCGCAGCCCCTCTGGATGCTCTTCAGCCTGCAT
  GGCGCCATCACGCAGGTCCGCATCCTCGGCTCCACCATCATGACTGAACGGGGTGGCCCATCTCTCCCCT
  GCTCACCTGCCTCCACTCCAACCTCACCCAGTGCCCTGGGCATCCGCCTCTCTGACCCCCTGCTCAGCAC
  CTGCGGTTCTGGGCCCCTAGGTGGCTCTGCTGGAGGGACAGCCCCCAACTCACCTGTGAGCCTGCCCGAG
  TCACCGGTGACCCCAGGTCTGGGCCAGTGGAGTGATGAATGCACCATTTGCTATGAACACGCAGTGGACA
  CAGTCATCTACACGTGTGGCCACATGTGCCTGTGCTACTCCTGTGGCCTGCGCCTCAAGAAGGCCCTGCA
  CGCCTGCTGCCCCATCTGCCGTCGCCCCATCAAGGACATCATCAAGACCTACCGCAGCTCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163480
• Insert Size: 1674 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163480.1, NP_001156952.1
• RefSeq Size: 3690 bp
• RefSeq ORF: 1674 bp
• Locus ID: 18011
• UniProt ID: Q923S6
• Cytogenetics: 19 39.39 cM
• Gene Summary: Plays a role in hippocampal-dependent synaptic plasticity, learning and memory. Involved in the formation of spines and functional synaptic contacts by modulating the translational activity of the cytoplasmic polyadenylation element-binding protein CPEB3. Promotes ubiquitination of CPEB3, and hence induces CPEB3-dependent mRNA translation activation of glutamate receptor GRIA1 and GRIA2. Can function as an E3 ubiquitin-protein ligase to activate monoubiquitination of JAG1 (in vitro), thereby regulating the Notch pathway. Acts as a tumor suppressor; inhibits malignant cell transformation of medulloblastoma (MB) cells by inhibiting the Notch signaling pathway.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.