Alk (NM_007439) Mouse Tagged ORF Clone

CAT#: MG221949

Alk (tGFP-tagged) - Mouse anaplastic lymphoma kinase (Alk), (10ug)

Product Images

Circular map for MG221949

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Alk
• Synonyms: CD246; Tcrz
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221949 representing NM_007439
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGCTGCTGGGTTCCTGTGGCTGCTGCCTCCACTGCTTTTGGCAGCAGCCTCGTACTCCGGAGCTG
  CAACCGATCAGCGCGCGGGTTCCCCAGCCTCAGGGCCTCCTCTGCAGCCCCGGGAGCCGCTCAGTTATTC
  GCGCCTGCAGAGGAAGAGTCTGGCAGTGGACTTCGTTGTACCCTCGCTCTTCCGCGTCTATGCCCGAGAC
  CTGCTGCTACCGCAGCCACGGTCCCCCTCGGAGCCCGAGGCTGGCGGGCTGGAGGCGCGGGGATCACTGG
  CCCTGGATTGTGAGCCTCTGCTCAGGCTGCTGGGGCCACTGCCTGGAATCTCCTGGGCAGATGGAGCCAG
  TTCTCCTAGTCCCGAGGCGGGTCCGACGCTGTCCAGGGTGCTGAAGGGAGGCTCGGTGCGCAAGCTCAGG
  CGTGCCAAACAGCTGGTGCTGGAGCTGGGCGAGGAGACGATTCTTGAAGGCTGTATTGGTCCCCCAGAGG
  AGGTAGCGGCTGTGGGGATACTCCAGTTCAACCTCAGCGAGCTGTTCAGCTGGTGGATTCTCCACGGCGA
  AGGGAGGCTGAGGATCCGCCTGATGCCTGAGAAGAAGGCATCGGAAGTGGGCAGGGAGGGAAGGCTATCC
  AGTGCGATCCGAGCCTCCCAGCCCCGCCTTCTCTTCCAGATCTTCGGGACGGGACACAGCTCCATGGAGT
  CACCCTCAGAAACGCCTTCTCCTCCTGGTACCTTCATGTGGAATCTGACCTGGACGATGAAAGACTCCTT
  CCCTTTCCTTTCCCACCGCAGTCGATATGGTCTGGAGTGCAGCTTTGACTTCCCCTGTGAGCTGGAATAT
  TCTCCTCCCCTGCACAACCACGGGAATCAGAGCTGGTCCTGGCGCCATGTGCCCTCCGAGGAGGCCTCGA
  GGATGAACTTGCTGGATGGGCCAGAGGCAGAGCATTCTCAAGAGATGCCCAGAGGCTCCTTCCTCCTCCT
  GAACACCTCTGCAGATTCCAAGCATACCATTCTGAGCCCATGGATGAGGAGCAGTAGTGATCACTGCACA
  CTGGCTGTCTCCGTGCACAGACATCTACAGCCTTCGGGGAGATATGTTGCCCAGCTCCTACCCCACAACG
  AAGCTGGAAGAGAGATTCTTTTGGTGCCCACCCCAGGGAAGCATGGCTGGACAGTGCTGCAGGGGAGAGT
  CGGGCGCCCAGCAAACCCATTTCGAGTGGCTCTGGAATACATCTCCAGTGGCAACCGGAGCTTGTCGGCG
  GTGGATTTCTTTGCCCTGAAGAACTGCAGTGAAGGGACATCCCCAGGCTCCAAGATGGCATTGCAGAGTT
  CCTTCACTTGTTGGAATGGGACCGTCCTCCAGCTCGGGCAAGCCTGTGATTTCCACCAGGACTGTGCCCA
  AGGAGAAGATGAGGGCCAGCTGTGCAGTAAACTTCCTGCTGGATTTTACTGTAACTTTGAAAATGGCTTC
  TGTGGCTGGACCCAAAGTCCACTCTCACCCCATATGCCCCGGTGGCAAGTGAGGACCCTAAGAGATGCCC
  ATTCCCAGGGCCACCAAGGCCGTGCCCTGTTGCTCAGCACCACTGACATCCTCGCTTCTGAAGGTGCAAC
  AGTGACCAGTGCCACCTTCCCTGCACCAATGAAAAATTCTCCTTGTGAGCTCCGCATGTCCTGGCTCATC
  CGCGGGGTTTTGAGAGGAAACGTATCTCTGGTGCTGGTGGAGAACAAAACCGGAAAGGAGCAAAGCCGGA
  CTGTCTGGCATGTCGCCACTGACGAAGGCTTAAGCCTGTGGCAGCATACAGTGCTGTCCCTCCTCGATGT
  GACTGACAGGTTCTGGCTGCAGATAGTCACATGGTGGGGTCCAGGATCCAGGGCAACCGTGGGATTTGAC
  AACATTTCCATCAGCCTCGACTGCTACCTTACCATCAGTGGAGAGGAGAAAATGTCCCTGAATTCAGTAC
  CCAAATCTAGAAATCTGTTTGAGAAAAACCCAAACAAGGAGTCAAAATCCTGGGCAAACATATCAGGACC
  AACTCCCATCTTCGACCCTACAGTTCACTGGCTGTTCACCACGTGTGGGGCCAGTGGACCTCATGGCCCC
  ACCCAGGCACAGTGCAACAACGCCTACCAGAATTCCAACTTGAGCGTGGTGGTGGGAAGTGAAGGGCCCT
  TGAAGGGAGTCCAGATTTGGAAAGTGCCAGCTACTGACACCTACAGTATCTCGGGCTACGGAGCAGCTGG
  CGGGAAAGGTGGGAAAAACACCATGATGCGGTCCCATGGCGTGTCTGTCCTGGGCATCTTCAATCTGGAG
  AAAGGTGACACACTCTACATCCTTGTCGGTCAGCAAGGGGAGGATGCCTGTCCCAGGGCAAACCAACTAA
  TCCAGAAAGTCTGTGTGGGTGAGAACAATGTCATAGAAGAAGAGATCCGAGTGAACAGAAGCGTGCACGA
  GTGGGCAGGAGGAGGAGGAGGTGGGGGTGGAGCCACCTACGTGTTTAAGATGAAAGATGGCGTGCCTGTA
  CCCCTGATCATTGCAGCTGGTGGTGGTGGCAGGGCCTATGGGGCCAAGACAGAAACGTTCCACCCAGAGA
  GACTGGAGAGTAACTCCTCGGTTCTAGGGCTGAACGGCAATTCCGGAGCCGCAGGTGGTGGAGGCGGCTG
  GAATGATAACACTTCCTTGCTCTGGGCCGGAAAGTCTTTGCTGGAGGGTGCCGCCGGAGGACATTCCTGC
  CCCCAGGCCATGAAGAAGTGGGGGTGGGAGACAAGAGGGGGTTTCGGAGGGGGTGGAGGGGGGTGCTCCT
  CAGGTGGAGGAGGCGGAGGATATATAGGTGGCAACGCAGCATCAAACAATGACCCCGAAATGGATGGGGA
  AGATGGGGTTTCCTTCATCAGTCCATTGGGTATCCTGTACACCCCGGCCTTAAAAGTGATGGAGGGCCAC
  GGGGAAGTGAATATCAAGCATTATCTAAACTGCAGTCACTGCGAGGTAGACGAATGTCACATGGACCCCG
  AGAGCCACAAAGTCATCTGCTTCTGTGATCATGGGACCGTGCTGGCTGATGATGGTGTCTCCTGCATTGT
  GTCACCCACCCCGGAGCCCCACCTGCCGCTCTCATTGATCCTCTCCGTCGTGACCTCTGCCCTGGTGGCT
  GCCCTTGTTCTGGCATTCTCCGGCATCATGATTGTGTACCGTCGGAAGCACCAGGAGTTGCAGGCTATGC
  AGATGGAACTGCAGAGCCCCGAGTATAAGCTGAGCAAGCTACGGACCTCGACCATCATGACCGACTACAA
  CCCCAACTACTGCTTCGCTGGCAAGACTTCCTCCATCAGTGACCTGAAAGAAGTGCCACGGAAAAACATC
  ACACTCATCCGGGGCCTAGGCCATGGCGCATTTGGGGAGGTGTATGAAGGCCAGGTGTCTGGAATGCCCA
  ATGACCCAAGCCCTCTACAAGTGGCTGTAAAGACGCTGCCAGAAGTGTGTTCAGAACAAGATGAGCTGGA
  CTTTCTCATGGAAGCTCTGATCATCAGCAAATTCAACCACCAGAATATTGTTCGCTGCATCGGGGTGAGT
  CTACAAGCCCTGCCCCGCTTCATCCTGCTGGAACTCATGGCTGGCGGAGACCTCAAGTCCTTCCTCAGGG
  AGACACGCCCTCGCCCGAACCAACCCACCTCCCTGGCCATGCTGGACCTTCTGCATGTGGCTCGGGACAT
  TGCCTGTGGCTGTCAGTACCTAGAGGAGAATCACTTTATCCACCGGGATATTGCTGCTAGAAACTGTCTG
  TTGACCTGCCCAGGAGCTGGAAGAATAGCAAAGATTGGAGACTTTGGGATGGCCCGAGATATCTACAGGG
  CCAGCTACTACCGAAAGGGAGGCTGCGCCATGCTGCCGGTCAAGTGGATGCCCCCTGAAGCCTTCATGGA
  AGGGATATTTACCTCTAAAACAGACACATGGTCTTTTGGAGTGTTGCTATGGGAAATATTTTCTCTTGGA
  TATATGCCGTACCCCAGCAAGAGCAACCAGGAAGTTCTGGAGTTTGTCACCAGCGGAGGACGGATGGACC
  CGCCTAAGAACTGCCCCGGGCCTGTATACCGGATAATGACGCAGTGCTGGCAGCATCAGCCTGAAGACAG
  ACCCAACTTCGCCATCATTTTGGAGAGGATCGAATACTGCACCCAGGACCCCGATGTGATCAACACAGCT
  CTGCCCATCGAATACGGTCCAGTAGTAGAAGAGGAGGAGAAAGTGCCCATGCGCCCCAAAGACCCCGAGG
  GGATGCCACCTTTGCTGGTGTCTCCCCAGCCTGCGAAGCACGAGGAGGCGTCCGCAGCTCCCCAGCCCGC
  AGCCCTGACGGCACCAGGCCCATCGGTGAAGAAGCCCCCGGGTGCGGGTGCGGGCGCGGGCGCGGGTGCG
  GGTGCCGGCCCGGTGCCCCGAGGTGCGGCCGATCGGGGCCACGTGAACATGGCTTTCTCTCAGCCCAACC
  CTCCCCCGGAGCTGCACAAAGGCCCGGGATCCAGAAACAAGCCGACCAGCCTGTGGAACCCCACCTACGG
  CTCGTGGTTCACCGAGAAGCCTGCCAAAAAGACCCATCCTCCGCCAGGCGCCGAGCCGCAGGCGCGGGCA
  GGAGCGGCCGAGGGTGGCTGGACCGGGCCGGGCGCGGGGCCCCGCAGAGCCGAGGCAGCGCTGCTGCTAG
  AGCCATCGGCGCTGAGCGCCACCATGAAGGAGGTGCCGCTGTTCAGGCTGCGCCACTTCCCCTGCGGCAA
  TGTCAACTATGGTTACCAGCAACAGGGTCTCCCCTTGGAAGCCACAGCCGCGCCAGGGGACACCATGCTG
  AAAAGCAAGAATAAGGTCACCCAGCCGGGGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221949 representing NM_007439
  Red=Cloning site Green=Tags(s)
  
  MGAAGFLWLLPPLLLAAASYSGAATDQRAGSPASGPPLQPREPLSYSRLQRKSLAVDFVVPSLFRVYARD
  LLLPQPRSPSEPEAGGLEARGSLALDCEPLLRLLGPLPGISWADGASSPSPEAGPTLSRVLKGGSVRKLR
  RAKQLVLELGEETILEGCIGPPEEVAAVGILQFNLSELFSWWILHGEGRLRIRLMPEKKASEVGREGRLS
  SAIRASQPRLLFQIFGTGHSSMESPSETPSPPGTFMWNLTWTMKDSFPFLSHRSRYGLECSFDFPCELEY
  SPPLHNHGNQSWSWRHVPSEEASRMNLLDGPEAEHSQEMPRGSFLLLNTSADSKHTILSPWMRSSSDHCT
  LAVSVHRHLQPSGRYVAQLLPHNEAGREILLVPTPGKHGWTVLQGRVGRPANPFRVALEYISSGNRSLSA
  VDFFALKNCSEGTSPGSKMALQSSFTCWNGTVLQLGQACDFHQDCAQGEDEGQLCSKLPAGFYCNFENGF
  CGWTQSPLSPHMPRWQVRTLRDAHSQGHQGRALLLSTTDILASEGATVTSATFPAPMKNSPCELRMSWLI
  RGVLRGNVSLVLVENKTGKEQSRTVWHVATDEGLSLWQHTVLSLLDVTDRFWLQIVTWWGPGSRATVGFD
  NISISLDCYLTISGEEKMSLNSVPKSRNLFEKNPNKESKSWANISGPTPIFDPTVHWLFTTCGASGPHGP
  TQAQCNNAYQNSNLSVVVGSEGPLKGVQIWKVPATDTYSISGYGAAGGKGGKNTMMRSHGVSVLGIFNLE
  KGDTLYILVGQQGEDACPRANQLIQKVCVGENNVIEEEIRVNRSVHEWAGGGGGGGGATYVFKMKDGVPV
  PLIIAAGGGGRAYGAKTETFHPERLESNSSVLGLNGNSGAAGGGGGWNDNTSLLWAGKSLLEGAAGGHSC
  PQAMKKWGWETRGGFGGGGGGCSSGGGGGGYIGGNAASNNDPEMDGEDGVSFISPLGILYTPALKVMEGH
  GEVNIKHYLNCSHCEVDECHMDPESHKVICFCDHGTVLADDGVSCIVSPTPEPHLPLSLILSVVTSALVA
  ALVLAFSGIMIVYRRKHQELQAMQMELQSPEYKLSKLRTSTIMTDYNPNYCFAGKTSSISDLKEVPRKNI
  TLIRGLGHGAFGEVYEGQVSGMPNDPSPLQVAVKTLPEVCSEQDELDFLMEALIISKFNHQNIVRCIGVS
  LQALPRFILLELMAGGDLKSFLRETRPRPNQPTSLAMLDLLHVARDIACGCQYLEENHFIHRDIAARNCL
  LTCPGAGRIAKIGDFGMARDIYRASYYRKGGCAMLPVKWMPPEAFMEGIFTSKTDTWSFGVLLWEIFSLG
  YMPYPSKSNQEVLEFVTSGGRMDPPKNCPGPVYRIMTQCWQHQPEDRPNFAIILERIEYCTQDPDVINTA
  LPIEYGPVVEEEEKVPMRPKDPEGMPPLLVSPQPAKHEEASAAPQPAALTAPGPSVKKPPGAGAGAGAGA
  GAGPVPRGAADRGHVNMAFSQPNPPPELHKGPGSRNKPTSLWNPTYGSWFTEKPAKKTHPPPGAEPQARA
  GAAEGGWTGPGAGPRRAEAALLLEPSALSATMKEVPLFRLRHFPCGNVNYGYQQQGLPLEATAAPGDTML
  KSKNKVTQPGP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007439
• ORF Size: 4863 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007439.2, NP_031465.2
• RefSeq Size: 5918 bp
• RefSeq ORF: 4866 bp
• Locus ID: 11682
• UniProt ID: P97793
• Cytogenetics: 17 43.77 cM
• Gene Summary: Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.[UniProtKB/Swiss-Prot Function]