H1f4 (NM_015787) Mouse Tagged ORF Clone
CAT#: MG216630
- TrueORF®
Hist1h1e (tGFP-tagged) - Mouse histone cluster 1 H1e (Hist1h1e), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_015787" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | H1f4 |
Synonyms | H1-4; H1.4; H1e; H1s-; H1s-4; H1v; H1var2; Hist1h; Hist1h1e |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG216630 representing NM_015787
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGAGACCGCGCCTGCTGCGCCCGCCGCACCGGCCCCCGCCGAGAAGACACCCGTCAAGAAGAAGG CCCGCAAGGCCGCAGGTGGCGCGAAGCGCAAAACCTCCGGACCCCCGGTGTCCGAACTCATCACCAAGGC TGTGGCCGCCTCCAAGGAGCGCAGCGGCGTGTCCCTGGCTGCGCTCAAGAAGGCGCTGGCGGCCGCGGGG TACGATGTGGAGAAGAACAACAGCCGCATCAAGCTCGGCCTGAAGAGCCTGGTGAGCAAGGGTACCCTGG TGCAGACCAAGGGCACCGGCGCCTCCGGCTCCTTCAAACTCAACAAGAAGGCGGCTTCCGGTGAGGCTAA GCCCAAAGCAAAAAGGGCAGGCGCGGCCAAGGCGAAGAAGCCTGCGGGCGCAGCCAAGAAGCCCAAGAAG GCTGCGGGGACAGCCACCGCCAAGAAGAGCACCAAGAAGACTCCAAAGAAAGCGAAGAAGCCAGCTGCAG CTGCAGGAGCCAAAAAAGCTAAGAGCCCGAAGAAGGCAAAGGCAACTAAGGCTAAGAAGGCGCCCAAGAG CCCTGCCAAGGCGAAAACGGTAAAGCCTAAAGCGGCCAAACCAAAAACCTCCAAGCCTAAGGCAGCTAAG CCAAAGAAAACCGCAGCCAAGAAAAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG216630 representing NM_015787
Red=Cloning site Green=Tags(s) MSETAPAAPAAPAPAEKTPVKKKARKAAGGAKRKTSGPPVSELITKAVAASKERSGVSLAALKKALAAAG YDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEAKPKAKRAGAAKAKKPAGAAKKPKK AAGTATAKKSTKKTPKKAKKPAAAAGAKKAKSPKKAKATKAKKAPKSPAKAKTVKPKAAKPKTSKPKAAK PKKTAAKKK TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_015787 |
ORF Size | 657 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015787.4, NP_056602.1 |
RefSeq Size | 1931 bp |
RefSeq ORF | 660 bp |
Locus ID | 50709 |
UniProt ID | P43274 |
Cytogenetics | 13 A3.1 |
Gene Summary | Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. [provided by RefSeq, Aug 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209859 | Hist1h1e (untagged) - Mouse histone cluster 1, H1e (Hist1h1e), (10ug) |
USD 330.00 |
|
MR216630 | Hist1h1e (Myc-DDK-tagged) - Mouse histone cluster 1, H1e (Hist1h1e) |
USD 300.00 |
|
MR216630L3 | Lenti ORF clone of Hist1h1e (Myc-DDK-tagged) - Mouse histone cluster 1, H1e (Hist1h1e) |
USD 600.00 |
|
MR216630L4 | Lenti ORF clone of Hist1h1e (mGFP-tagged) - Mouse histone cluster 1, H1e (Hist1h1e) |
USD 600.00 |
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