Abhd3 (NM_134130) Mouse Tagged ORF Clone

CAT#: MG206458

Abhd3 (tGFP-tagged) - Mouse abhydrolase domain containing 3 (Abhd3)

Product Images

Circular map for MG206458

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Abhd3
• Synonyms: AA675331; LABH3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206458 representing NM_134130
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCGCCTGGCCATGGACCTGCGGGTGCTGTCCCGGGAGCTGGCGCTCTACCTGGAGCACCAGGTTC
  GCGTCGGCTTCTTCGGCTCGGGCGTGGGCTTGTCACTGATCTTGGGATTCAGCGTTGCCTATGCTTGCTA
  CTACCTGAGTAGCATTGCCAAGAAACCCCAGTTAGTGATTGGAGGGGAGAGTTTCAGCCGCTTCCTACAG
  GACCACTGTCCCGTGGTGACAGAAACCTATTACCCGACGGTCTGGTGCTGGGAGAGCCGAGGACAGACAT
  TGCTGAGACCCTTCATCACTTCCAAACCCCCGGTGCAATACAGGAACGAACTCATTAAAACTGCAGACGG
  AGGACAGATCTCACTGGACTGGTTTGATAACAATAACAGTGCGTACTATGTGGATGCCAGCACCAGACCT
  ACTATCTTGCTCTTGCCTGGCCTCACTGGAACAAGCAAGGAATCCTACATCCTTCATATGATCCATCTCA
  GTGAAGAATTAGGATACAGGTGTGTGGTTTTTAATAACAGAGGAGTAGCAGGAGAAAGTCTCTTGACACC
  ACGGACTTACTGCTGTGCGAACACTGAAGACTTGGAGGCCGTCGTCCACCACGTGCACAGCCTGTACCCT
  GGGGCTCCTTTTCTGGCAGCAGGCGTATCAATGGGAGGAATGCTGCTTCTGAATTACTTGGGTAAAATTG
  GGTCCAAAACCCCTCTGATGGCTGCTGCAACGTTTTCGGTTGGCTGGAACACTTTCGCTTGCTCAGAGTC
  TCTGGAGAGGCCGCTGAACTGGCTACTTTTTAATTACTACTTAACAACTTGCCTCCAGTCATCAGTTAAG
  AAGCACCGGCATATGTTTGTGGAGCAGATTGACATGGATCAGGTCATGAAGGCCAAGTCGATCAGAGAGT
  TTGATAAGCGGTTCACGGCGGTCATGTTTGGGTACCGCACACTCGATGATTACTACACCGATGCCAGTCC
  AAACCGCAGGCTGAAGTCAGTGGGAATCCCTGTTCTGTGCCTGAATGCTACGGACGACGTGTTCTCACCC
  AGCCATGCTATCCCAATAGAAACTGCTAAACAAAACCCTAACGTGGCTTTGGTCCTTACAGCCTATGGAG
  GCCATATTGGTTTTCTGGAGGGAATCTGGCCTAGGCAATGCACTTACATGGATCGAGTCTTCAAGCAATT
  TGTACAGGCCATGGTTGAGCACGGACATGAACTCTCCAACATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206458 representing NM_134130
  Red=Cloning site Green=Tags(s)
  
  MQRLAMDLRVLSRELALYLEHQVRVGFFGSGVGLSLILGFSVAYACYYLSSIAKKPQLVIGGESFSRFLQ
  DHCPVVTETYYPTVWCWESRGQTLLRPFITSKPPVQYRNELIKTADGGQISLDWFDNNNSAYYVDASTRP
  TILLLPGLTGTSKESYILHMIHLSEELGYRCVVFNNRGVAGESLLTPRTYCCANTEDLEAVVHHVHSLYP
  GAPFLAAGVSMGGMLLLNYLGKIGSKTPLMAAATFSVGWNTFACSESLERPLNWLLFNYYLTTCLQSSVK
  KHRHMFVEQIDMDQVMKAKSIREFDKRFTAVMFGYRTLDDYYTDASPNRRLKSVGIPVLCLNATDDVFSP
  SHAIPIETAKQNPNVALVLTAYGGHIGFLEGIWPRQCTYMDRVFKQFVQAMVEHGHELSNM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134130
• ORF Size: 1233 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134130.1, NP_598891.1
• RefSeq Size: 1903 bp
• RefSeq ORF: 1236 bp
• Locus ID: 106861
• UniProt ID: Q91ZH7
• Cytogenetics: 18 A1
• Gene Summary: Phospholipase that may play a role in phospholipids remodeling. May selectively cleave myristate (C14)-containing phosphatidylcholines through its predominant phospholipase 1 activity, cleaving preferentially acyl groups in sn1 position. In parallel, may have a minor phospholipase 2 activity acting on acyl groups in position sn2. In addition to (C14)-containing phosphatidylcholines, may also act on other medium-chain-containing and oxidatively truncated phospholipids.[UniProtKB/Swiss-Prot Function]