Gdap1l1 (NM_144891) Mouse Tagged ORF Clone

CAT#: MG205694

Gdap1l1 (tGFP-tagged) - Mouse ganglioside-induced differentiation-associated protein 1-like 1 (Gdap1l1)

Product Images

Circular map for MG205694

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gdap1l1
• Synonyms: Gdap1l
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205694 representing NM_144891
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCCCCAACAACCTGACCCCCACCAACTGCAGCTGGTGGCCCATCTCGGCACTGGAGAGCGATG
  CAGCCAAGCCGGTGGAGACCCCCGACGCGCCCGAGGCATCCAGCCCCGCCCATTGGCCCAAGGAGAGCCT
  GGTTCTGTACCACTGGACCCAGTCCTTCAGCTCGCAGAAGGTGCGGCTGGTGATCGCTGAGAAGGGCCTG
  GCCTGTGAAGAGAGAGATGTGAGCCTGCCTCAGAGCGAGCATAAGGAACCGTGGTTCATGCGGCTCAACC
  TGGGTGAGGAGGTCCCAGTCATTATCCACAGAGACAACATTATCAGTGACTATGACCAGATCATTGACTA
  CGTGGAACGCACCTTCACGGGAGAGCATGTGGTGGCCTTGATGCCCGAGGCGGGCAGCCCACAACACGCC
  CGAGTGCTGCAGTACCGGGAGCTGCTGGATGCGCTGCCCATGGACGCCTACACACACGGCTGCATCCTGC
  ACCCTGAGCTCACCACAGATTCCATGATCCCCAAGTATGCCACGGCTGAGATCCGCAGACATTTAGCCAA
  CGCCACCACAGACCTCATGAAACTGGACCACGAGGAGGAACCACAGCTCTCTGAGCCCTACCTTTCTAAA
  CAAAAGAAGCTTATGGCCAAGATCCTGGAGCACGATGACGTGAGCTACCTGAAGAAGATCCTCGGGGAGC
  TGGCTATGGTGCTGGACCAGATTGAGGCGGAGCTGGAGAAGAGGAAACTGGAGAATGAGGGGCAGACGTG
  CGAGCTGTGGCTCTGTGGCTGTGCCTTCACCCTGGCGGATGTCCTCCTGGGAGCCACCCTGCACCGCCTC
  AAGTTCCTGGGACTGTCCAAGAAATACTGGGAAGACGGCAGCCGGCCCAACCTGCAGTCCTTCTTTGAGA
  GGGTCCAGAGACGCTTTGCCTTCCGGAAAGTCCTGGGCGACATCCACACCACTCTGCTGTCAGCTGTTAT
  CCCCAACGCGTTCCGACTGGTCAAACGGAAACCGCCATCCTTCTTTGGGGCATCCTTCCTCATGGGCTCC
  TTGGGTGGGATGGGCTACTTTGCCTACTGGTACCTCAAGAAAAAATACATC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205694 representing NM_144891
  Red=Cloning site Green=Tags(s)
  
  MATPNNLTPTNCSWWPISALESDAAKPVETPDAPEASSPAHWPKESLVLYHWTQSFSSQKVRLVIAEKGL
  ACEERDVSLPQSEHKEPWFMRLNLGEEVPVIIHRDNIISDYDQIIDYVERTFTGEHVVALMPEAGSPQHA
  RVLQYRELLDALPMDAYTHGCILHPELTTDSMIPKYATAEIRRHLANATTDLMKLDHEEEPQLSEPYLSK
  QKKLMAKILEHDDVSYLKKILGELAMVLDQIEAELEKRKLENEGQTCELWLCGCAFTLADVLLGATLHRL
  KFLGLSKKYWEDGSRPNLQSFFERVQRRFAFRKVLGDIHTTLLSAVIPNAFRLVKRKPPSFFGASFLMGS
  LGGMGYFAYWYLKKKYI
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144891
• ORF Size: 1101 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144891.2, NP_659140.2
• RefSeq Size: 2580 bp
• RefSeq ORF: 1104 bp
• Locus ID: 228858
• Cytogenetics: 2 H3