Rgs5 (NM_009063) Mouse Tagged ORF Clone
CAT#: MG201631
- TrueORF®
Rgs5 (tGFP-tagged) - Mouse regulator of G-protein signaling 5 (Rgs5)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_009063" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Rgs5 |
Synonyms | 1110070A02Rik |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG201631 representing NM_009063
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTAAGGGACTGGCAGCTCTGCCGCACTCATGCCTGGAAAGGGCCAAGGAGATCAAGATCAAGTTGG GAATTCTCCTCCAGAAGCCAGACTCTGCTGTTGACCTTGTCATTCCATACAATGAGAAGCCAGAGAAGCC TGCCAAGGCACACAAGCCCTCACTGGAGGAGGTCCTGCAGTGGCGCCAGTCCCTGGACAAGCTTCTCCAG AACAGCTATGGATTTGCCAGCTTCAAAAGTTTCCTGAAGTCTGAATTCAGTGAGGAAAACCTTGAGTTCT GGGTTGCCTGTGAGAATTACAAGAAGATCAAGTCCCCCATCAAAATGGCGGAGAAGGCAAAGCAAATTTA TGAAGAATTCATCCAGACAGAGGCCCCTAAAGAGGTGAACATTGACCACTTCACTAAAGACATCACCATG AAGAACCTGGTGGAACCGTCTCCTCGCAGCTTTGACTTGGCCCAGAAAAGGATCTATGCCCTGATGGAGA AGGATTCTCTGCCCCGCTTTGTGCGCTCTGAATTTTATAAGGAGCTAATCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201631 representing NM_009063
Red=Cloning site Green=Tags(s) MCKGLAALPHSCLERAKEIKIKLGILLQKPDSAVDLVIPYNEKPEKPAKAHKPSLEEVLQWRQSLDKLLQ NSYGFASFKSFLKSEFSEENLEFWVACENYKKIKSPIKMAEKAKQIYEEFIQTEAPKEVNIDHFTKDITM KNLVEPSPRSFDLAQKRIYALMEKDSLPRFVRSEFYKELIK TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_009063 |
ORF Size | 543 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009063.4 |
RefSeq Size | 1810 bp |
RefSeq ORF | 546 bp |
Locus ID | 19737 |
UniProt ID | O08850 |
Cytogenetics | 1 76.84 cM |
Gene Summary | Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205544 | Rgs5 (untagged) - Mouse regulator of G-protein signaling 5 (Rgs5), (10ug) |
USD 300.00 |
|
MR201631 | Rgs5 (Myc-DDK-tagged) - Mouse regulator of G-protein signaling 5 (Rgs5) |
USD 300.00 |
|
MR201631L3 | Lenti ORF clone of Rgs5 (Myc-DDK-tagged) - Mouse regulator of G-protein signaling 5 (Rgs5) |
USD 600.00 |
|
MR201631L4 | Lenti ORF clone of Rgs5 (mGFP-tagged) - Mouse regulator of G-protein signaling 5 (Rgs5) |
USD 600.00 |
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