Nudt1 (NM_008637) Mouse Tagged ORF Clone

CAT#: MG201165

Nudt1 (tGFP-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 1 (Nudt1)

Product Images

Circular map for MG201165

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nudt1
• Synonyms: Mth1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201165 representing NM_008637
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCACCTCCAGGCTTTATACCCTTGTGCTAGTGCTACAGCCTCAGCGAGTTCTCCTGGGCATGAAGA
  AGAGGGGCTTTGGTGCTGGCCGCTGGAATGGCTTCGGGGGCAAGGTGCAGGAAGGAGAGACCATTGAGGA
  TGGGGCTAAGAGAGAGCTGCTGGAAGAAAGTGGTCTGAGCGTGGATACACTGCACAAGGTAGGCCACATC
  TCGTTTGAATTTGTGGGCTCCCCTGAGCTGATGGACGTGCATATCTTCTCGGCTGACCATGTGCACGGGA
  CGCCCACAGAGAGTGAAGAAATGCGCCCTCAGTGGTTCCAACTGGACCAGATCCCCTTTGCCGACATGTG
  GCCGGATGACAGCTACTGGTTCCCACTCCTGCTTCAGAAGAAGAAGTTCTGTGGGCACTTCAAGTTCCAG
  GATCAGGACACGATCCTCAGTTACTCGCTGCGAGAGGTGGACTCATTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201165 representing NM_008637
  Red=Cloning site Green=Tags(s)
  
  MSTSRLYTLVLVLQPQRVLLGMKKRGFGAGRWNGFGGKVQEGETIEDGAKRELLEESGLSVDTLHKVGHI
  SFEFVGSPELMDVHIFSADHVHGTPTESEEMRPQWFQLDQIPFADMWPDDSYWFPLLLQKKKFCGHFKFQ
  DQDTILSYSLREVDSF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008637
• ORF Size: 468 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008637.1, NP_032663.1
• RefSeq Size: 939 bp
• RefSeq ORF: 471 bp
• Locus ID: 17766
• UniProt ID: P53368
• Cytogenetics: 5 G2
• Gene Summary: Antimutagenic (PubMed:11572992). Plays a redundant role in sanitizing oxidized nucleotide pools, such as 8-oxo-dGTP pools (PubMed:29281266). Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress. Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP (By similarity). Does not play a role in U8 snoRNA decapping activity (PubMed:21070968). Binds U8 snoRNA (PubMed:21070968).[UniProtKB/Swiss-Prot Function]