Jpt1 (NM_008258) Mouse Tagged ORF Clone
CAT#: MG201138
- TrueORF®
Hn1 (tGFP-tagged) - Mouse hematological and neurological expressed sequence 1 (Hn1)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_008258" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Jpt1 |
Synonyms | Hn1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG201138 representing NM_008258
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCACTACCACTACCTTTAAGGGTGTGGACCCTAACAGCAGGAACAGCTCCCGGGTTTTGCGGCCTC CAGGTGGTGGGTCCAATTTTTCATTAGGCTTTGATGAGCCAGCAGAACAGCCTGTGAGGAAGAACAAGAT GGCTTCTAACATCTTTGGGACACCGGAAGAGAACCCCCCATCTTGGGCCAAGTCAGCAGGTTCCAAGTCT AGTGGTGGCAGGGAAGATTCGGAGTCGCCTGGAACACAGAGAAGTAACTCTTCTGAAGCAAGCTCTGGAG ATTTCTTAGACCTCAAGGGAGAAGGTGATATGCATGAAAATGTGGACACAGACTTCCAAGCCAACCTGGC GCAGATGGAGGAGAAGCCAGTGCCCGCTGCTCCTGTGCCCAGCCCAGTGGCTCCAGCCCCAGTGCCATCC AGGAGAAACCCCCCTGGCGGCAAGTCCAGCCTGGTCTTGGGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201138 representing NM_008258
Red=Cloning site Green=Tags(s) MTTTTTFKGVDPNSRNSSRVLRPPGGGSNFSLGFDEPAEQPVRKNKMASNIFGTPEENPPSWAKSAGSKS SGGREDSESPGTQRSNSSEASSGDFLDLKGEGDMHENVDTDFQANLAQMEEKPVPAAPVPSPVAPAPVPS RRNPPGGKSSLVLG TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_008258 |
ORF Size | 462 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008258.2 |
RefSeq Size | 1384 bp |
RefSeq ORF | 465 bp |
Locus ID | 15374 |
UniProt ID | P97825 |
Cytogenetics | 11 80.84 cM |
Gene Summary | Modulates negatively AKT-mediated GSK3B signaling. Induces CTNNB1 'Ser-33' phosphorylation and degradation through the suppression of the inhibitory 'Ser-9' phosphorylation of GSK3B, which represses the function of the APC:CTNNB1:GSK3B complex and the interaction with CDH1/E-cadherin in adherent junctions (By similarity). Plays a role in the regulation of cell cycle and cell adhesion (PubMed:25450365). Has an inhibitory role on AR-signaling pathway through the induction of receptor proteosomal degradation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200349 | Hn1 (untagged) - Mouse hematological and neurological expressed sequence 1 (Hn1), (10ug) |
USD 150.00 |
|
MR201138 | Hn1 (Myc-DDK-tagged) - Mouse hematological and neurological expressed sequence 1 (Hn1) |
USD 150.00 |
|
MR201138L3 | Lenti ORF clone of Hn1 (Myc-DDK-tagged) - Mouse hematological and neurological expressed sequence 1 (Hn1) |
USD 450.00 |
|
MR201138L4 | Lenti ORF clone of Hn1 (mGFP-tagged) - Mouse hematological and neurological expressed sequence 1 (Hn1) |
USD 450.00 |
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