Mkl2 (NM_181860) Mouse Tagged ORF Clone
CAT#: MG200325
- TrueORF®
Mkl2 (tGFP-tagged) - Mouse gene trap insertion site 4-1 (Gt4-1)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_181860" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Mkl2 |
Synonyms | ENSMUSG00000075401; Gt4-1; mKIAA1243; MRTF-B; Mrtfb |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG200325 representing NM_181860
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCACACAGGAGCCATAGACACAGAGGAAGAGCTGGGGCCTCTAGCCCATCTTGCACCAAGTCCTC AAAGTGAGTCTGTGGCTCACGAATTCCAGGAACTGTCTCTACAGTCCAGCCAGCACTTACCCACTCTGAA CGAACGGAAAAATGGAAGTATAGTCTTTGGGGAGTCAGGACTGCAGCTTCCTCTGCCGGCTCAGTCTGTG AGCACAGGACATAATAAGAAAAGCAAGAAGCCTCATTTGCCACTAGTCTTGGGTTCCTGCAGGCTGCATT TAGAACCTGCCAGGTGTTTGGATGGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG200325 representing NM_181860
Red=Cloning site Green=Tags(s) MDHTGAIDTEEELGPLAHLAPSPQSESVAHEFQELSLQSSQHLPTLNERKNGSIVFGESGLQLPLPAQSV STGHNKKSKKPHLPLVLGSCRLHLEPARCLDG TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_181860 |
ORF Size | 306 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_181860.1, NP_862908.1 |
RefSeq Size | 717 bp |
RefSeq ORF | 309 bp |
Locus ID | 239719 |
Cytogenetics | 16 A1 |
Gene Summary | Acts as a transcriptional coactivator of serum response factor (SRF). Required for skeletal myogenic differentiation.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC213083 | Mkl2 (untagged) - Mouse MKL/myocardin-like 2 (Mkl2), transcript variant 2, (10ug) |
USD 165.00 |
|
MR200325 | Mkl2 (Myc-DDK-tagged) - Mouse MKL/myocardin-like 2 (Mkl2), transcript variant 2 |
USD 165.00 |
|
MR200325L3 | Lenti ORF clone of Mkl2 (Myc-DDK-tagged) - Mouse MKL/myocardin-like 2 (Mkl2), transcript variant 2 |
USD 465.00 |
|
MR200325L4 | Lenti ORF clone of Mkl2 (mGFP-tagged) - Mouse MKL/myocardin-like 2 (Mkl2), transcript variant 2 |
USD 465.00 |
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