Rdbp (BC034867) Mouse Untagged Clone

CAT#: MC206563

Rdbp (untagged) - Mouse RD RNA-binding protein (cDNA clone MGC:41238 IMAGE:1495547), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rdbp
• Synonyms: RD, NELF-E
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC034867
  CGGGCCCGGGCGCCGCCCCCTCAGGAGCCAACATGTTGGTGATACCTCCGGGGCTGAGTGAAGAGGAAGA GGCACTGCAGAAGAAGTTCAACAAACTCAAGAAGAAGAAAAAGGCATTGCTGGCTCTGAAGAAGCAGAGC AGCAGCGGCCCAGCGAGCCAGGGCGGTGTCAAACGCTCACTGTCAGAGCAGCCTGTGGTGGACACTGCCA CAGCAACAGAACAGGCCAAACAACTGGTGAAGTCAGGAGCCATCAGTGCCATCAAGGCTGAAACTAAGAA CTCAGGCTTCAAACGTTCTCGGACCCTGGAAGGGAAGTTAAAGGACCCAGAGAAGGGGCCTGTCCCCACT TTCCAGCCGTTCCAGAGGAGCATGTCTGCTGATGAGGACCTACAGGAGCCATCCAGACGTCCCCAGAGGA AATCTCTGTATGAAAGCTTTGTGTCTTCTAGTGATCGGCTTCGGGAACTGGGACAAGATGGAGAAGAAGC AGAGGCCCCCGGGGCTGGTGATGGCCCACCTCGAGGCTTTGACTGGAGCTATGAAGAGCATGGTAGCGCC CGCTCCTCAGCCTCCCCTCCCCGAAGCCGCAGCAGGGACCGAAGTCATGACCGGAGCCGAGATAGGGATC GGGACAAAGAGAGAGACAGAGACCGAGATCGAGATCGAGACAGAGACCGGGATAAAGACAAAGACAGAGA CCGGGATCGAGACAGAGACCGGGACCGAGATCGGGAACGAGACCGAGAGGGCCCTTTCCGCAGGTCAGAC TCATTCCCCGAACGGAGGGCCCCTCGAAAGGGGAACACACTGTATGTGTATGGAGAGGACATGACGCCCA CCCTCCTCCGCGGGGCCTTCTCTCCCTTTGGAAATATCATCGACCTCTCCATGGACCCACCCAGAAACTG TGCCTTCGTCACCTACGAGAAGATGGAGTCTGCAGATCAGGCTGTTGCTGAGCTCAATGGGACCCAGGTG GAATCTGTGCAGCTTAAAGTCAACATAGCCCGAAAACAGCCCATGCTGGACGCTGCTACTGGCAAATCTG TCTGGGGCTCCCTTGCTGTCCAGAACAGCCCTAAGGGTTGCCATCGAGACAAGAGGACCCAGATTGTGTA CAGTGACGATCTATAGGAAACCTTGTGGATGGCTTCTAGGACATGAAGCTGGATTCCTTGTGCCTCATAT GCCCCCAAGCTGGTCTCAGTAAAATCCTGGGGTAGAAGCTTTTCACCTCCGTCAGCCTCTAGTCTTTCAG CACTTGGGATTGGAGTTAAGCCTTCAAAAGCGGCTGTCAGTGTTGATGTTAACCAGTGCACGCCCTTACC CCAAAAAGATGTGGAACACAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC034867
• Insert Size: 1104 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC034867, AAH34867
• RefSeq Size: 1372 bp
• RefSeq ORF: 1104 bp
• Locus ID: 27632
• Cytogenetics: 17 18.4 cM
• Gene Summary: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (By similarity). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (By similarity). Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA (By similarity).[UniProtKB/Swiss-Prot Function]