Lrrc8e (BC080783) Mouse Untagged Clone

CAT#: MC206426

Lrrc8e (untagged) - Mouse leucine rich repeat containing 8 family, member E (cDNA clone MGC:91131 IMAGE:30481669), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lrrc8e
• Synonyms: 1810049O03Rik; C87354
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC080783
  CTGATAGCGTCTGTAGTGAGGCGGGGCCGAACAATCCCGGGGGCGGGGCGGGGCGGCTCCTGGCCCAGCC CGCCCTGACTCACCCTCTCTGGCCCGCCCGGGAACTCTGCGGGAGTCCGGGTCGATCTACTCCCAGGCAT CTAGAAGTTGGGCCCCGGAGTTCCATCCCTGAGCAGTGAGCAGGATGATCCCAGTGGCGGAGTTCAAGCA GTTCACAGAGCAGCAGCCTGCATTCAAGGTGCTCAAACCCTGGTGGGATGTTCTGGCTGAGTACCTCACC ATGGCTATGCTCATGATCGGGGTATTCGGGTGCACCCTCCAGGTGACACAGGACAAGATCATCTGCTTGC CTAGCCACGAATCTCGTGAGAACATATCGGGGGCTCCATGCCAGCAGCTTTTGCCTCAAGGAATCTCTGA GCAGATGGGGGGTCTCCGAGAGCTCAGTGGCCTCAAAAACAACCTAGACCTCCAGCAGTATAGCTTTATC AACCAGCTCTGCTATGAGACTGCCCTCCACTGGTATGCCAAATACTTCCCCTACTTGGTGGTTATCCATA CTCTCATCTTTATGGTCTGTACCAGCTTTTGGTTCAAATTCCCTGGTACCAGCTCCAAAATAGAACACTT CATCTCCATCCTGGGCAAGTGCTTTGATTCTCCATGGACCACTCGGGCCTTGTCCGAGGTGTCTGGAGAA AATCACAAGGGCCCAGCTTCTGGACGGGCCATGGTGACCACGGTAACCACCACAGGGGCAGGGTCGGGGA AGGTGGGTGAAGGTGAGAAGGAGAAAGTGCTCATTGAGCCCGAAAAGGTGGTGTCTGAGCCTCCAGTTGT TACTCTGCTGGATAAGAAGGAGGGCGAGCAAGCGAAGGCCCTATTCGAGAAGGTCAAAAAGTTCCGAGTG CACGTGGAAGAGGGTGACATCCTCTATTCCATGTACATCCGGCAGACAGTGCTCAAAGTGTGTAAGTTTT TTGCCATCCTGGTTTACAACCTGATCTATGTGGAGAAGATCAGCTTTCTGGTGGCCTGTAGGGTGGAGAC ATCAGAGATCACGGGGTATGCCAGTTTCTGCTGCAACCACACCAAGGCCCACCTCTTTTCTAAGCTGGCC TTCTGCTATATTTCCTTTGTGTGTGTCTATGGCATCACCTGCCTGTATACACTCTACTGGCTCTTCCATA GGCCCCTAAAAGAGTACTCCTTCCGTTCTGTGCGTGAGGAGACAGGGATGAACGATATCCCGGATGTTAA GAATGACTTTGCCTTCATGCTCCACCTCATTGACCAGTACGACTCCCTCTATTCCAAACGCTTTGCAGTC TTCCTCTCTGAAGTCAGTGAGAGCCGCCTTAAGCAACTCAACCTCAACCATGAGTGGACACCTGAGAAGC TGAGGCAGAAGCTGCAGCGTAACATGCGTGGCCGGCTGGAGCTGAGCCTCTGCATGCTCCCTGGACTACC TGACACCGTCTTTGAGCTCAGCGAGGTGGAGGCACTCAGGCTGGAGGCCATCTGTGACATCTCCTTTCCC CCTGGGCTCTCCCAGCTTGTGAACCTTCAGGAGCTCAGCTTGCTTCACTCACCAGCCAGGCTGCCCTTTT CATCACAGATCTTCCTGCGAGATCGCCTGAAGGTGATCTGTGTTAAATTCGAGGAGCTCCGAGAGGTACC CCTCTGGGTGTTTGGGCTTCGAGGCTTAGAAGAACTGCACCTGGAAGGACTCTTTCCCCCAGAAATGGCT CGGGGTGCCACCCTGGAGAGCCTCCGGGAGCTTAAACAGCTCAAGGTACTGTCTCTACGTAGCAATGCAG GGAAGGTGCCAGCCAGTGTGACTGATGTGGCCGGGCACCTTCAGCGACTCAGCCTGCACAATGATGGGGC TCGTTTGCTTGCCCTAAACAGCCTCAAGAAGCTGGCAGTGTTACGGGAGCTGGAGCTGGTGGCCTGTGGG CTGGAACGTATCCCCCATGCAATCTTCAGCCTGGGGGCTCTGCAGGAACTTGACCTTAAGGACAACCACC TTCGGTCTATCGAGGAGATCCTCAGCTTCCAGCACTGTCGGAAGCTGGTCACTCTCAGGCTGTGGCACAA CCAGATTGCCTATGTGCCCGAGCATGTGAGAAAGCTCCGGAGCCTGGAGCAGCTCTACCTCAGTCACAAC AAGCTGGAGACTCTGCCCACCCAGCTGGGCCAGTGCTTTGGCCTCCGCCTGCTGGATTTATCCCACAACG GGCTTCGTTCCCTGCCACCTGAGTTGGGCCTTCTCCAGAGCCTCCAGCATCTAGCCCTCTCCTACAACGC ACTCGAGAGTCTGCCCGATGAATTGTTCTTCTGCCACAAGCTGCGGACGTTACTCTTGGGCTACGACCAC CTAACTCAGCTCTCACCTGACGTGGCTGCCCTCCAGGCCCTCAGCCGTCTGGGGCTCAAGGGTAACAGGT TGGAGACACTGCCAGAAGAACTTGGTGACTGCAAGGGGCTGAAGAAGTCGGGGCTGCTGGCGGAGGACAC CCTTTATGAGGGACTGCCTGCAGAAGTTCGGGAAAAGATGGAGGAGGAATGAAGCCTGGTGGGGGAAGGG GCAGGCTGACACAGAGGCCTTTACATGTTTCTGCCCAACATTGTATTCCAAGGGAGAGAGGTCCAGCAGG AGTGGAAGGAAGGCAGGACATGTCGGTTTTGTGCAGCTCAGACAAGATCCTGGGACTGGTTTTTCTGGAG GAGAAACAGGAGGCGGTAGATTTGAGGTGTAACCCCTTAGGGAAGGATTAACCAGTCTTAGGTTCTTCAA CCAAAACCACACCTGTGTCTTTGGCTGGCTGGCTTGCCCTTCCTCTGAACATTCTGGCTTAATTAGTTTC CTATATGGGAGTGGGATTTCCAACCTTTCCCAACAAGGATCAGCATATACTTAGGGTTCTCTCCTAAGGA GAGGATACAGGGTCCAGTTCCAAAGTTCCATATCCCAACTTTCATGCAAGTCCTGATTTTATAAGCCCTT TGTGACAGGGATGGGTACTTAGAACAATGGCTCTCACATTTAGCTATGTAATAAAATCAGCTGAGAGGCC CGGAAGAAAACAAAACCCTTACATGCTTCCCACCCCACCCCTGAAATTATACTGACTCAGAATTTGGATG GGTAGCTGAGCATGGTGGCACACGCCTTTAATTCCAGCACTCAGGAGGCAGAGGCAGGTGGATTTCTGAG TTCGAGGCCAGCCTGGTCTACAAAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCAAAA AAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC080783
• Insert Size: 2388 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC080783, AAH80783
• RefSeq Size: 3308 bp
• RefSeq ORF: 2388 bp
• Locus ID: 72267
• Cytogenetics: 8 A1.1
• Gene Summary: Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine. Mediates efflux of amino acids, such as aspartate, in response to osmotic stress. Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition.[UniProtKB/Swiss-Prot Function]