Mylip (BC010206) Mouse Untagged Clone

CAT#: MC206229

Mylip (untagged) - Mouse myosin regulatory light chain interacting protein (cDNA clone MGC:11702 IMAGE:3964381), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mylip
• Synonyms: MGC11702, Mir
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC010206
  GGAGCAATTGCGGTGAGGCGACAGCCCCGGCGCACACCCGAGAAGAAGCGGCGGTGGCGGCGGCCCCAGC CATGCTGTGCTATGTGACGAGGCCGGACGCGGTGCTGATGGAGGTAGAGGTGGAGGCAAAAGCCAACGGC GAGGACTGTCTCAACCAGGTGTGCAGGCGTCTAGGGATCATCGAGGTTGATTATTTTGGGCTGCAGTTCA CGGGGAGCAAAGGTGAGAGCTTATGGCTGAATCTGAGAAACCGGATCTCCCAGCAGATGGATGGGCTGGC ACCTTACCGCCTTAAACTGAGGGTCAAGTTCTTTGTGGAGCCTCATCTCATCTTACAGGAGCAGACAAGG CATATCTTTTTCTTGCACATTAAAGAGTCCCTCTTGGCAGGCCACCTCCAGTGTTCTGCAGAGCAGGCCG TGGAACTCAGTGCCCTCCTGGCTCAGACCAAATTTGGAGACTACAACCAGAACACCGCCCAATACAGCTA TGAGGACCTGTGTGAGAAAGAGCTCTCCAGCTCCACTTTGAACAGCATCGTTGCGAAGCATAAGGAGCTG GAGGGCATCAGCCAGGCCTCTGCCGAGTACCAGGTTCTGCAGATTGTGTCAGCGATGGAGAACTACGGCA TAGAGTGGCATGCTGTGAGGGACAGCGAAGGACAGAAACTCCTCATTGGGGTCGGACCTGAAGGCATCTC GATCTGTAAAGAGGACTTTAGCCCTATTAACAGGATAGCTTATCCTGTGGTGCAGATGGCCACCCAGTCA GGAAAGAATGTCTACTTGACCGTCACCAAGGAGTCCGGCAACAGCATCGTGCTCCTGTTTAAGATGATCA GCACCAGAGCAGCCAGCGGCCTCTACCGAGCCATCACCGAAACACATGCATTCTATAGGTGTGACACAGT CACCAGTGCCGTCATGATGCAGTACAGTCGCGACCTGAAGGGCCACTTGGCGTCTCTGTTTCTGAACGAA AACATTAACCTTGGTAAGAAATACGTCTTCGACATCAAGAGAACATCCAAAGAGGTCTATGACCATGCCA GGAGGGCTCTGTACAACGCCGGCGTTGTGGACCTTGTCTCTCGAAGTGACCAGAGCCCCCCCAGCTCACC CCTGAAGTCCTCAGACAGCAGCATGAGCTGCAGCAGCTGTGAGGGCCTCAGCTGCCAGCAGACCCGGGTG CTGCAGGAGAAGCTGCGCAAGCTGAAGGAAGCCATGCTGTGTATGGCGTGCTGCGAGGAGGAGATCAACT CCACCTTCTGCCCCTGCGGCCACACTGTGTGCTGCGAGAGCTGTGCAGCCCAGCTGCAGTCCTGTCCGGT CTGCAGATCCCGTGTGGAGCATGTCCAGCACGTCTACCTGCCCACCCACACCAGTCTCCTCAATCTGACT GTCATCTGATGCGTCCTGCACTCGATGGACAAGCCATGTCCCCACAAGCTGCAGTATTGTAAACTATAAG AATAATAACTTTGTGAAGAGCTATTTCACTCTCAACACCCATCTGCCATGAGACATTTTCAGAAACAAGG AGGAAAAGAAAACAAGAATGTGACCACACCTCTTCCGTGAGGAGAAGCAACAGGCCCCATGGCCACCAGG AAGAACTCTGGGACACGGACACATTCCTTGAACTTTAGGGTTGGTTTTTTTTTTAATGATCAAGTAAAGG AGTAGATAGAATCGTCTTCGTCAGTCAAGTGGCAACATGGCCCAACCGTGGGCACCTTTTAGGAAATGAC GTCATATGTCTCCTTCACTTTTTCCCCGGGCAGCATTTTGTAAGTGTTTTAAGGATTTCCTTGGTTCTTT TTGTATGGTCATGGAGCGCTGAATATTTTTAATAGGGATTTTTTTTCTTAAAGAAATAGTCCTCATTATA AAAGTCATTTCTGTCTTTATAACTCATTCAAGAACAACTGGAAAAGCTGGCAGATTGAAAAAAAAAAAGC AATCCTGTGACTTCCCAAGGGTTGACAGCAATGTTGTCAGATTGGAAGCAGTCTGGCTGAGAGCCAATAG GTAACTCACCGTGGGTGACTTCCTTCCTAGAGCCCTTCCGTTTCCCCTCATTCCACACCCCATGCCTTTC ACTGATAAAAATGCTACCAGTTTGGTTAAGAGACATACATGGTAGAGTCAAGCACTCCCTGGGCTTTGGA GATTGGAAAGCGAGAGTAGCTTCCTTGAAGGAAAAAGATGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG AGAGAGAGAGAGAGAGAGAGAGAGAGATGAGCCAGAGAGCCACTCAGTATGCCCGAGTGGTTCTTCACTT TCCCAAGCACTCACTCCAGCTGCACCCATGGGTGTCGCCTTGCTTGAAGATCAAACTTTCTACAGCCTTA TAGGTTTCTAGATAGTGTCTCCTTTTTGTGTATGTCTTGTTTCTCGTTGTTCGAGTTTTCCTATGTCAGT GCTTCCATACTCATTGTCCTGCCCCCTCGGTGTCTTCCAGAGGTAGGGCTACTTCTTTATGTTTCCATAT TCTAAGTTTTCACCCCCACTTGGGCATTTTGGATGCTAGTGAGCTAGGGGGTTTTCTAGGGTGTCAGGAA ACCTAGCTGACCTCATCGGGTGCAATACTAGCTAAGTTAAAGCTAGAAGCCTACACTGTCACTTTACTGA GATTTCTGAGTCTACGTTTCATATTGCCTTAATGTAGCAGTAATGTGTTTATGCATTTGTTTCTTTGCAC AGACATTTTGTCAGATATTAAAACTCTACTTTTTTATGGCACATATTAGCATATAAGCCTTTATTCCAAG AGGTATTTATTTTTTCACTTGTAAAAAAAATAATGTTTCCACATTTAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC010206
• Insert Size: 1338 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC010206, AAH10206
• RefSeq Size: 2859 bp
• RefSeq ORF: 1338 bp
• Locus ID: 218203
• Cytogenetics: 13 A5
• Gene Summary: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.[UniProtKB/Swiss-Prot Function]