E130012A19Rik (BC055770) Mouse Untagged Clone

CAT#: MC206061

E130012A19Rik (untagged) - Mouse RIKEN cDNA E130012A19 gene (cDNA clone MGC:67345 IMAGE:6403695), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: E130012A19Rik
• Synonyms: AA409164; AI413509; AW539173; E13; esPRC2p48
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC055770
  GGAGCCAGCGGGGAAGAAAGAAGCTGAGAGGGCGGAAGGCGCCCTCCCCGCCGCTGGGCCCGGGCACCGG GCGCCGGGCCGGGGTCCTTCCCGCCGGCGGCGAGCGCCGGCTGCTTTGTTTGCCCCCGCCTCGGCGTTGA GGGGTGATCGTGCCGGGCATTGCGCCGGGAACGCGGCGGCAGGAAGCGCGACCGATCGCACCGGGGACGC GCGGGGACCCAGGAGTCCGGCCGCGGCGAGGGCCGCGTGACGATACCGAGGAGAGAGCCGGAGCGCCGCC GCGGGCTCTTGGTGGTGGGCCCCGGACGCCCGTGAGCACAGACCTCCCCGTCGACGGGGGGCGATGTTCC CCTGGCCCGCAGGCTCTTCGGGCCGCCGGGGTATCCCGCCGTTGGGACCAGGGCCCCCGCAGTGGGCGAC CCCCAAGGCTGCTCCATGGAGACTCTGTGTCCTCCTCCCCGCCTGGCAGTGCCCGCTTCCCCGCGGGGGT CTCCCTGCTCGCCTACGCCCCTGAAGCCGCGTCGGGGGACCCCAGAGTTCTCCCCACTGTGCCTGCGTGC CCTCGCCTTCTGCGCCCTTGCCAAACCCCGGCCGTCCTCCCTGGGCCTGGGGCCTGGAGAGCTGGCGCCG CGGACCCCGGTGCTGCTGGGCCCCCCGGCCTCGCCATGCACAGGAGGCTGGGCCGCGGACGGCCTGAAGC ACCTCGGGGGACAGGCCGGGCGGCCGAGCGACGTGAGCTCCCCGGCCCGCGAGGATGCAGACGTCGCGGT GTGCCCAGGCGGTGGAGAGGAGGAAGAGGGCGGAGGCGGCTTCCCGCACTTCGGCGCTGGTTCCTGCGCA CCTCCGGGCCGCTGCCCTGCTCCCCTGCGTCCTCAGGACTCTCCGACCAACCCCGCCTGGTCCCCGCCCA GGCCAGCCCGGGGTCTTGATGCAGCTAGCAGTCCGCCCCTTGAGCCCGGCTCCCCGCCTCCGTCGCCACC TGCGGGCCTCTCCCCGGAGCCTGCGCCTTCCGAGCAGCCTGTGCCGGCCTCCGAGGCTCCAGGGGGTGGA GACCCTGCGCCGACCGCGCCTGAGGCACCCGCGCTCAGCCCCTCGACGGCAGACGCAGCACCGGACCCGC CCCGCGATCTCCGCCAGGAACATTTCAACCGCCTGATCCGCCGCTCGAAACTTTGGTGTTACGCGAAGGG GTTCGCCCTGGACACTCCAAGTTTGCGCCGAGGGCCCGAACGGCCCGCGGCCAAAGCCCGTGGAGCAGCC AAGAAACGACGGCGGCCAGCGCCGCCCCCGCCCAGTGTGCAGCCACGGCGGCCCGTCCCGACGCTTCCTA CATCGAGCACCTTCAGCCTCCTTGACTGCTTCCCCTGTCCCCCAGCTCTGGTGGTGGAAGAGAACGGAGA CCTAGGGCCGGCTTCCTCGCTTCGCCTCCAGGGAGACGCCAAGCCGCCGCCTGCCCACCCGCTGTGGAAG TGGCAGATGGGAGGACCCGCTGTCCCTGAGCCCCCGGGTCTCAAATCCTGGTGGGTCAACTTGGAAGAAC TATGAGCGTCGGACCTCTTCTACCCGGGGGCAAGTTGGGTTCCCCGGACAGACTGAGGGCTCCAGGAGGG AGCATCGATTCTGAAATTTGGCTGATCTTTGCTTTGCATTTTATGGGCTGTGGCCCGCGGGACCTCAAAT GACAGTGATCTTCAAGCACCCGAGAGGTCCGGCTGATGCTCTTTCTACTCATCTTTGTGGAAGATCCAAG GACTGGCGTCCGGGAAAGCCTGCAGAAAGCTTAGTGACCATCTCCGTCCTCCCTGCGGGCGGGCAGAGCC TTCACCGCAGGGGAGCACAGCGGCTAAGGCCCGAAGACCAAAAGAAAGGGACAGAGGGACGGACCTTGGT GATGGACAAACCGGTTGTTTCCATGTTTGAATGAGGGTCAGTTTAGCGGTGTGGGGGCTGTGGTTGGGGT TTGAAGGTGATTGACAGCTCCCTGGGGGCATCCCCCACCCCCCACTGCCCAGACCTTTAACCCTTTACAC CTCCCTCTTTTCTCTCCGTAGTCCTCCCCCTCCCCAAGTTCAGGTGATTTTCCCTTGTCAAGATCTTCAG TCCCAGCCAGCAGACTTTACCCTTTTGTCCAGTTTTGGAGTTTCCCTTTGCCGGAAGGTTGGATGAAGGA AAGAGACCTCCAGCCTCAGCGTTCTCCTCACATTCTGAGAGGAAAGGAGGAAGAGGGCCATGCTGTGTGG CCCAAGGCTGGCTTTGCCCGGGGAAGGAGCCAAAAACTCCCGGGGAAAATGATGGAAGATGGAATAAATC AATAAAATCAGACCAAACAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC055770
• Insert Size: 1110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC055770, AAH55770
• RefSeq Size: 2349 bp
• RefSeq ORF: 1110 bp
• Locus ID: 103551
• Cytogenetics: 11 D
• Gene Summary: Scaffold protein that serves as a bridging partner between the PRC2/EED-EZH2 complex and the elongin BC complex: required to fine-tune the transcriptional status of Polycomb group (PcG) target genes in embryonic stem cells (ESCs) (PubMed:27863225, PubMed:27863226). Plays a key role in genomic regions that display both active and repressive chromatin properties in pluripotent stem cells by sustaining low level expression at PcG target genes: acts by recruiting the elongin BC complex, thereby restricting excessive activity of the PRC2/EED-EZH2 complex (PubMed:27863225, PubMed:27863226). Interaction with USP7 promotes deubiquitination of H2B at promoter sites (PubMed:27863226). Acts as a regulator of neuronal differentiation (PubMed:23180766).[UniProtKB/Swiss-Prot Function]