Sae1 (NM_019748) Mouse Untagged Clone

CAT#: MC205310

Sae1 (untagged) - Mouse SUMO1 activating enzyme subunit 1 (Sae1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sae1
• Synonyms: 2400010M20Rik; 2610044L12Rik; AL033372; AOS1; AW743391; D7Ertd177e; HSPC140; Sua1; Uble1a
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC068164
  CGAGAGCGGGAGCGGGTGAGCTGAAAGCCGGCGCCATGGTAGAGAAGGAGGAGGCTGGCGGCGGCGGCGG CGGTGGCATCAGCGAGGAGGAGGCAGCGCAGTATGACCGACAGATCCGCCTGTGGGGACTGGAGGCCCAG AAGCGGCTTCGAGCTTCCCGGGTGCTGATTGTTGGCATGAAAGGACTTGGAGCTGAAATTGCCAAGAATC TTATCCTGGCAGGAGTCAAAGGGCTCACCATGCTGGACCACGAACAGGTATCTCCAGAAGACCCTGGAGC TCAGTTCTTGATTCAGACTGGGTCTGTGGGCCGCAATAGGGCCGAGGCCTCTTTGGAACGAGCCCAGAAT CTCAATCCCATGGTGGATGTAAAGGTGGACACTGAGGATGTAGAGAAGAAGCCAGAGTCCTTCTTCACGA AGTTTGATGCGGTGTGCCTGACTTGCTGCTCCAGAGATGTCATCATTAAAGTCGACCAGATCTGTCACAG AAACAGCATTAAGTTCTTCACAGGAGATGTCTTTGGGTACCATGGGTACACCTTTGCGAATCTTGGAGAG CATGAATTTGTAGAAGAGAAAACCAAAGTTGCCAAAGTAAGCCAAGGAGTGGAAGATGGACCTGAGGCCA AGAGAGCAAAGCTTGATTCATCAGAGACAACCATGGTCAAAAAGAAAGTGCTTTTTTGCCCTGTAAAAGA GGCGCTAGAAGTGGACTGGAGTGGAGAGAAAGCCAAGGCTGCCCTGAAGCGTACTGCCCCCGACTACTTT CTGCTGCAAGTGCTGCTGAAATTCCGCACAGATAAAGGGAGAGATCCGACTTCTGAGAGCTACAAGGAGG ATGCGGAGCTGCTGCTGCAGATACGGAATGACGTGTTTGACTCCCTGGGCATCAGTCCTGACCTGCTTCC CGATGACTTTGTCAGGTACTGCTTCTCTGAGATGGCCCCAGTATGTGCTGTGGTTGGAGGGATCTTGGCA CAGGAAATTGTGAAGGCCCTGTCTCAAAGGGACCCTCCTCACAACAACTTCTTCTTCTTCGACGGCATGA AGGGGAGTGGAATTGTGGAGTGCCTTGGTCCCCAGTGAGCTGAGGTGTGTACAAGGCCACCGAGTCACCT GCCCTCTCTCCGGATCATTTTTCTAGAGCAAGCAGGCCAGACTTCATCCTGCCTTTGTCTTCGAAGCTAT CAGGGAACTGCGGGGTCCCAAATATGATAATCACGTGGCACCTCTGGCATTGCTCCCTTGGGCTGTCACT GGGGACTGGCAGTGCCTAGTGAGAGAGAGCATCATACTTCATGATGTCAGAGTGTCTTGTCACGGTTTGG ACTTAACTTCCCTCTATAGTCAGCAGAGGGGATCCGAGGCCCAGTGCTGCCTGGAGAACTGAGAATAGAC CTCTGTTGTTGACCCTTCCTCAGGCTGGAGCCTGGGTGTTGGAAGGGAGAGACTGTCACCCAGTTGTCAT GTCTTTTGGTTGTGAGCCTATGCACCTTACCTTTAATGGTTGAACCATCTTTTCAGCCCAAACAGTTTAT TTCTAAAAAGTAGATTTAATGCTAGGGGCCATTATGCAGGCTTGTAATCCTAGATGTTGGGATGCCGGGG CAGGATTTACTGTAAATTTCAGGGCCAAGTTGGTCTACATAATGTACCAAGAACCTTTCTCAAAATATAA TAAAGGGCATATTCCAGAGTTAGGGTGGGGCACTGTTACCCAGCTCCTTATACTGGGCACCCGGGCCCCT GACAGGAGTGGGTTGGGGGGCAGTTGGTTTGGTCTTCCTTCTAGGAGTATGAGGGCAGCCTGTCTAGTTT GTAGATCCCCTTGGTTGCTGGATAAAGGATGATAATAAAGTGTCTTTCTCTTAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_019748
• Insert Size: 1053 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC068164, AAH68164
• RefSeq Size: 1906 bp
• RefSeq ORF: 1053 bp
• Locus ID: 56459
• UniProt ID: Q9R1T2
• Cytogenetics: 7 8.88 cM
• Gene Summary: The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1 and 2 both encode the same isoform (a).