Plcd4 (NM_001081456) Mouse Untagged Clone

CAT#: MC204781

Plcd4 (untagged) - Mouse phospholipase C, delta 4 (Plcd4), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Plcd4
• Synonyms: 4921507K24Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC066156
  TCGCAGACACAAGATTACTATCCTACCTACGGTGACTCTGGCACCAGCTGGTGGAGCAGTGGGTGATGAC ATCTCAGATTCAAGACCTACTAGCCACTGATCAGGACCTGCTGCTGATGCAAGAAGGCACAATGATGCGC AAGGTGAGGACCAAAAGCTGGAAGAAGCTAAGATACTTCAGACTTCAGAATGACGGCATGACAGTCTGGC ATGGAAGTCAACCAGAAAGCATGCCCAAGCCCACTTTTTCGATCTCTGATGTGGAGAGGATACGTAAGGG GCAGGATTCTGAGTTGTTGCGCTATCTGGTGGAGGAGTTTCCCCTGGAGCAAGGGTTCACCGTTGTCTTT CATGGGCGCCGCCCCAACCTGGACCTAGTGGCCAACAGTGTTGAAGAGGCCCAGATTTGGATGCGAGGAC TCCAGCTGTTGGTGGATCTTGTTGCCAGCATGGACCACCAGGAGCAAATGGATCAAATGTTGAATGAGTG GTTCCAGCAAGCAGACAGAAACCAGGATGGCCGGATGAGTTTCAGAGAAGCTCAACGGCTGCTGCTTCTG ATGAACGTGGAAATGGATGAAGAGTACGCCTTCAGTCTTTTCCAGGAGGCAGATGTTACCCAGTCTGATG ATCTGGGGTCGGAAGAATTTGTACAGTTCTATAAGGCCCTGACTAAGCGCACTGAGATTGAAGAAATATT TGAGGACTTCTCATCTGACAAACAGAAGCTGACCTTGCTGGAATTTGTGGATTTCCTTAGAAAGGAACAG AAAGAAAAAGACCATGCTCCTGACCTCGCTCTGGAACTCATTGACCGCTATGAGCCTTCTGAGAATGGCC GATTGCTGCATGTGCTGAGCAAGGATGGCTTCCTCAAGTACCTCTGCTCGAAGGATGGAAACATCTTCAA CAGTGACTGCCTCCCTATCTACCAGGATATGACTCAGCCTCTGAGCCACTACTACATTAACTCTTCTCAC AACACCTACCTAGCGGGGGACCAGCTTTGCGGCCAGAGCAGCGTTGAAGGATACATACGGGCCTTGAAAC GGGGGTGCCGCTGTGTGGAGGTGGACACGTGGGATGGACCTGATGGAGAGCCTGTTGTTTATCATGGGCA TACCCTGACCTCTCGCATCCTATTCAAAGATGTGTTGGCAACTCTCGCGCAATATGCCCTCCAGTCATCA GACTACCCTCTCATCTTGTCTCTGGAAAACCACTGCACCTGGGAACAGCAGAGGACCATGGCACACCATC TGACCGAGATCCTGGGAGAGCAGCTGCTGAGGAACACCCTAGAGGGATTGCTGGTTGACAGCATGCCCTC GCCAGAGCAGCTTCGGGGGAAGATCTTGGTGAAAGGAAAGAAGTTAAGAACAATTGAAGTTGACAAGGAA GAAGAAGAAGAAGAAGAAGAAGAAGAGCTGGAGAAAGATGAGGGGCCAGATCTGGACCCAGCCTCCCCTG AGTTGGACACTCAACCTCAGCCTGAGACCCAGGGGCAGGCCGCTGGGAACAAGAAGGAGAGGAAGAAGAA GTTTCTTCTCCTGAGTTCCACAACCATCATGTGCCCAGACCTATCTGCCCTGGTTGTCTACTTGAGGACT GTCCCCTTCTGCAGCTTCACTCATTCCAAAGAAAACTACCACATCTATGACATTTCATCCTTTTCTGAGT CCAAGGCCAAGAACCTCATCAAGGAGGCAGGCAACGAGTTTGTACAGCACAATGCCAGACAGTTATGCTG CGTGTATCCCAGTGGTCTGAGGACAGATTCATCCAACTTTAACCCCCAGGAACACTGGAATGTAGGCTGC CAGATGGTGGCTATGAATATGCAGACTGCAGGAAGTGCAATGGATATCTGTGATGGGCTCTTCCGCCAGA ATGGTGGCTCTGGCTATGTGCTGAAGCCAGAATTCCTGAGAGATACCCAGAGTTCATTCAATCCTGAGAG GCCAATTAGTCTTTATAAGGCCCAGATCCTTGTAGTCCAGGTGATCAGTGGCCAACAACTCCCCAAAGTG GATAAAACCAAAGAGACAACCGTTGTGGATCCACTGGTGAAAGTAGAGCTCTATGGTGTTCCTGAAGACA CAAAGGAGCAAGAGACCAGCCATGTGGAGAACAATGGTATTAATCCATACTGGGGGGAGACTTTCTATTT CCGGCTCCAGGTACCTGAACTTGCTATGTTGCGTTTTGTGGTAAAGGATTATAGCAGGAAATCCCGAAAT AACTTTATTGGCCAATACACCCTACCTTGGACCTGTATGAAACAAGGCTACCGACATGTATCCTTGCTCT CCAGAGATGGCACTAGCCTCAATCCAGCTTCCATCTTTGTATACACCTGCATGCAGGAAGACCTGGATAT GGATGAGCCCTGAGAAACACAGGGAGGGCCTTGAAGAACAATCTACAGATGCACAATCCTTTCCAACCTA CAACTTCCTCAAAATCCAGAGCCAGCCAAAGGATCAATGAAGGATCAAGGTTGCCAGGCAAAGGTTGAGG AAGAATGCCTGACTCAAAACTGTGACTCTAGAGAACAATGCACAGCCCTGAGACCCCACTGGACTGCTGC TCCTTAGCTGGTCTAAATAGAGCTTCTCTGTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_001081456
• Insert Size: 2328 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC066156, AAH66156
• RefSeq Size: 2658 bp
• RefSeq ORF: 2328 bp
• Locus ID: 18802
• UniProt ID: Q8K3R3
• Cytogenetics: 1 38.54 cM
• Gene Summary: Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca(2+) mobilization in the zona pellucida-induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter protein (isoform 1).