Ncapd2 (NM_146171) Mouse Untagged Clone

CAT#: MC204239

Ncapd2 (untagged) - Mouse non-SMC condensin I complex, subunit D2 (Ncapd2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ncapd2
• Synonyms: 2810406C15Rik; 2810465G24Rik; CAP-D2; CNAP1; mKIAA0159
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC026982
  GTATCCTGCCCTATTGTTTTCTTCCTTCCTTTTCAGCAGGGGGCTCTCCGGTTGAAGAAGCGGCGGGTGA GGGGTGTCGCGGGGTCCAGGTGAACCAGGGGGAGAAAGATGTCTCCCCACAACTTTGAATTCCACTTGCC CTTATCTCCAGAGGAGCTGCTGAAAAGTGGAGGTGTGAATCAGTACGTTGTCCGGGAGGTGCTGCCCGTC AAGCACCTCTCATCCCAGCTTAGAGCATTTCAGTCTGCCTTCCGAGCTCAGGGGCCCCTCGCCATACTGG AGCACTTCGATACAGTCTACAGCATTTTACATCACTTTCGAAGTATAGAACCTGGCCTCAAGGAAGACAC TCTGGAATTCCTGAAGAAAGTGGTATCCCGCCACTCCCAGGAACTGTCGTCCATCCTGGATGATGCAGCT CTGAGTGGATCAGATAGAAGCGCCCACCTGAATGCCCTCAAAATGAACTGCTATGCCCTGATACGCCTTT TGGAGTCCTTTGAGAATATGACCAGCCAGACAAGCCTCATTGACCTGGACATCGGTGGGAAGGGTAAGAG AGCCCGGGCCAAAGCAACCCTTGGCTTTGACTGGGAGGAAGAGAGGCAACCAGTTCTTCAGCTTTTAACA CAGCTGCTCCAGCTGGACATCCGTCACCTATGGAACCACTCAGCAATTGAAGAAGAATTTGTCAGTTTGG TTACTGGCTGTTGCTACCGCCTTTTGGAGAATCCCACCATTAGTCACCAGAAGAACCGCTCCACCAAGGA GGCCATAGCACACCTGCTTGGGGTGGCCTTGGTCCGCTACAACCATATGCTCAGTGCCACTGTGAAGATC ATCCAGATGTTGCAGCACTTTGAGCACCTGCCCCCTGTGTTGGTGACAGCCGTGAGTCTGTGGGCAACAG ACTACGGGATGAAGAGCATAGTGGGAGAGATTGTAAGAGAGATTGGACAGAAGTGTCCACAGGAGCTGAG TCGAGACACAGCAGGGGCAAAGGGCTTTGCGGCTTTCCTCACTGAGCTAGCAGAGCGAATCCCAGCTGTC CTGATGGCCAACATGTGCATTCTGCTAGACCATCTGGATGGAGAGAATTACATGATGCGCAATGCTGTGT TAGCAGCTATAGCCGAGATGGTACTGCAGGTTCTAAATGGTGACCAGCTCGAAGAATCAGCTCGAGAAAC CAGAGATCAGTTCTTGGACATCTTGCAAGCTCATGGCCATGATGTGAACTCTTTTGTTCGGAGCCGTGTT TTGCAGCTCTTTGCCCGAATTGTCCAACAGAAGGCACTCCCTCTGACCCGTTTCCAGGCAGTGGTGGCCT TGGCAGTGGGACGGCTGGCAGACAAGTCAGTGCTAGTGTGTAAAAATGCCATTCAGCTGCTGGCCAGCTT TCTAGCCAATAATCCCTTTTCCTGCAAGCTTAGTGATATTGACCTTGCTGGACCACTGCAGAAGGAGATT CAGAAATTACAAGAAATGCGAGCCCAGAGGCGAAGTGCAGCAGCGACTGCAGCACTAGACCCGGAGGAGG AGTGGGATGCCATGTTGCCAGAATTGAAGTCGACCCTGCAGCAGCTCCTGAAACTTCCCCAGGAAGAGGG AGATCATCAAATTGCTGATGCAGAGACTGCAGAGGAGGTGAAAGGACGCATCCGCCAACTGCTTGCCAAA GCAAGTTACAAACAGGCTATTGTTCTCACTCGGGAAGCTACCAGTCACTTCCAGGAGTCTGAACCCTTCA GTCACACAGAGCCAGAGGAGAACAGCTTCCTGAATCTCTTGGGACTCATCTTCAAAGGCCCCGAAGCTTC CACACAGGGCTCTCATGGGGACACAGACCCAGGGCTGACAGGCAGTAAAGACTCTCCCAGTGTGCCCGAG CCAGAGGGATCCCAGAGCAACGATGAGCTGGTGAAGCAGGAGATGCTGGTGCAGTATCTGCAGGATGCCT ACGGCTTCTCCCAGAAGATTACAGAAGCCATTGGCATCATCAGCAAGATGATGTATGAGAACACAACTAC AGTGGTGCAGGAGGTGATTGAGTTCTTCGTGATGGTATTCCAATTTGGGGTACCTCAGGCCCTGTTTGGG GTACGCCGCATGCTGCCACTCATCTGGTCTAAGGAGCCTGGTGTCCGGGAAGCCGTGCTTAATGCGTACC GCCAACTCTACCTCAACCCGAAAGGGGACTCAGCCAGAGCCAAGGCGCAGACTTTGATTCACAACCTCTC GCTGTTGTTAGTGGATGCCTCCGTCGGGACCATTCAGTGTCTTGAGGAAATTCTTTGTGAGTTTGTGCAG AAGGATGAGGTAAAGCCAGCGGTGATCCAGCTGCTATGGGAACGGGCAACGGAGAAGGTGCCAAGCTCTC CTCTGGAGCGCTGCTCCTCTGTTATGCTTCTTGGCATGATGGCACGAGGAAAACCAGAAATTGTAGGGAG CAACTTAGACGCCCTGGTGAGGGTGGGGCTGGATGAGAAGTCTCCCCAGGACTACAGGCTGGCCCAGCAG GTGTGCCTTGCCATTGCTAACATCTCGGACAGGAGGAAGCCTTCCCTGGGTGAGCGTCACCCTCCCTTCC GGCTGCCTCAGGAGCACAGGTTGTTTGAGCGACTTCAGGATATGGTCACAAAGGGCTTTGCACACCCAGA TCCCCTCTGGATCCCATTCAAAGAAGTAGCAGTGACCCTGACCTACCAGTTGGCAGAGAGCCCTGATGTG CTCTGTGCCCAGATGTTGCAAGGCTGTGCCAAGCAAGTCTTGGAGAAACTTGAGAAGAACGCCACTGAGG CAGACCCCAAGGAAACAGCCCCTAGGCTCCCCACATTCCTGCTGATGAACCTGCTGTCCCTGGCTGGGGA TGTGGCTCTACAGCAGCTGGTGCATTTGGAACAAGCAGTGAGTGGAGAGCTGGGTCGGCGGCGAGTTCTG CGAGAGGAGCAGGAGCACAGGGCCAAGGAGCCCAAGGAGAAGACTGCAAGCTCTGAAACTACAATGGAGG AAGAACTGGGGCTGGTGGGGGGGGCCACTGCTGACGACACAGAGGCGGAGCTCATCCGAAGCATCTGTGA GAAGGAGCTGTTGGACGGCAACCAGGTACTAGCTGCCTTTGTTCCACTGCTACTTAAAGTCTGTAACAAC CCTGGCTTATACAGCAACCCAGAGCTCTGTGCGGCTGCGTCGCTGGCCCTTGGCAAGTTCTGCATGATCA GTGCCCCTTTCTGCGACTCCCAGCTTCGTCTTCTATTCACCATGCTGGAAAAGTCTTCACTCCCCACTGT GCGATCTAACCTCATGGTTGCCACAGGGGATCTGGCTATCCGCTTCCCTAACCTGGTGGATCCCTGGACT CCTCATCTGTATGCCCGCCTCCGAGACCCAGCTCAACAAGTGCGGAAGACAGCGGGGCTGGTGATGACGC ACCTGATCCTGAAGGACATGGTGAAGGTGAAGGGGCAGGTGAGCGAGATGGCCGTGCTGCTCATCGACCC TGTGCCTCAGATCGCTGCTCTGGCCAAGAATTTCTTCAATGAGCTCTCCCACAAGGGCAATGCTATCTAT AATCTACTCCCCGATATCATCAGTCGCCTGTCAGACCCAGAGGGCGGAGTGGAAGAGGAGCCCTTCCACA CCATCATGAAGCAGCTCCTCTCCTACATCACCAAGGACAAACAGACTGAGAGCTTGGTAGAAAAGCTGTG TCAGCGGTTCCGCACAGCCCGAACTGAGCGTCAGTACCGAGACCTAGCCTACTGTATGTCCCAGCTGCCC CTCACAGAGCGAGGTCTCCAGAAGATGCTAGACAACTTTGAGTGCTTTGGCGATAAGCTCTTAGATGAGT CTGTCTTCAGTGCTTTCTTGTCAGTTGTGGGCAAACTGCGTCGCGGGGCCAAGCCTGAGGGCAAGGCTAT AATAGATGAATTTGAGCAGAAGCTTCGTGCCTGTCACACCAGAGGCATGGATGGAATAGAGGAGTTTGAA ACTGGCCAGGGAGGCAGCCAGCGAGCCCTGTCTGCCAAGAAACCCTCCGCTGTCTCAAGGCTACAGCCTC TGACTTCTGTAGACTCAGACAATGACTTTGTCACACCTAAGCCGCGACGTACCAAACCTGGTCGTCCACA AACTCAGCAGCGCAAGAAGTCCCAGAGGAAAGCCAAAGTTGTCTTCTTGAGTGACGAGTCCAGTGAGGAC GAACTTTCAGCAGAGATGACAGAAGAAGAGACACCCAAGAGAACCACCCCCATCCGCAGAGCATCTGGGC GAAGACACAGGTCCTAGGAGCTGCCTTTGCCTACTCCCGTCCCTGCCGTGCAGGGTATCTTACTGGATGA TCTTGGATTCAGTGCCCCCCCCCCCTTGTAGAAGTGCTTGTTGTCTGTGGTTTTAACATGGAAATGGCCT TTGCATTGAGGTCTTTCTCTTAGAGCCCTGCTGCCACTTTCCCTTTCTCCGAGCCTGTGCCAATTGCTGT TTTCTAATGAATAAATGTTTTTATAGACTTTTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_146171
• Insert Size: 4179 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026982, AAH26982
• RefSeq Size: 4527 bp
• RefSeq ORF: 4179 bp
• Locus ID: 68298
• UniProt ID: Q8K2Z4
• Cytogenetics: 6 F2- F3
• Gene Summary: Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. May target the condensin complex to DNA via its C-terminal domain. May promote the resolution of double-strand DNA catenanes (intertwines) between sister chromatids. Condensin-mediated compaction likely increases tension in catenated sister chromatids, providing directionality for type II topoisomerase-mediated strand exchanges toward chromatid decatenation. Required for decatenation of non-centromeric ultrafine DNA bridges during anaphase. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size.[UniProtKB/Swiss-Prot Function]