Fzd2 (NM_020510) Mouse Untagged Clone

CAT#: MC203551

Fzd2 (untagged) - Mouse frizzled homolog 2 (Drosophila) (Fzd2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fzd2
• Synonyms: AL033370; AW456835; Fz10; Fzd10; Mfz10; Mfz10a
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC055727
  TGGGGAGAGGGGAAGCCCAGAGGCTAGAGGAGGAAGAGGAGCTAGGGCAGCGGTGCGCCGCGTCTCCGAC CCTCAGTCCGACCGCGGCGAGCCAGCAGGCGGGTGCGCCGCCCCCTCCCCCGCCGCCCAATCTCCCCAAC TCTGCGGCCGCGAGCAAAGTTTGCAAGGAGACGCGGGAGGCGGCCGGGCAGCTAGGCGGCAGCGGGGAAG GCGCGCGGTCTCTGGGTTGGGGGCGGGGGCTGGGGGGCGCCCAGGAGCCGAGTGGGGGGCGGCGGCCAGC ATGCGGGCCCGCAGCGCCCTGCCCCGCAGCGCCCTGCCCCGCCTGCTGCTGCCACTGCTGCTGCTGCCGG CCGCCGGACCGGCCCAGTTCCACGGGGAGAAGGGCATCTCCATCCCGGACCACGGCTTCTGCCAGCCCAT CTCCATCCCGCTGTGCACGGACATCGCCTACAACCAGACCATCATGCCCAACCTTCTTGGCCACACGAAC CAGGAAGACGCGGGCCTGGAGGTGCATCAGTTCTACCCGCTGGTGAAGGTGCAGTGCTCGCCCGAGCTGC GCTTCTTCCTGTGCTCCATGTACGCGCCGGTGTGCACAGTGCTGGAGCAGGCCATCCCGCCGTGCCGCTC CATCTGCGAGCGCGCGCGCCAAGGCTGCGAGGCGCTCATGAACAAGTTCGGCTTCCAATGGCCCGAGCGC CTCCGCTGCGAGCATTTCCCGCGTCACGGCGCGGAGCAGATCTGCGTGGGCCAGAACCACTCGGAGGACG GAGCTCCTGCGCTACTCACCACCGCGCCACCTTCTGGGCTGCAGCCCGGCGCGGGTGGCACCCCGGGCGG CCCTGGCGGTGGTGGCTCGCCACCGCGTTACGCCACTCTGGAGCACCCTTTCCACTGTCCCCGCGTCCTC AAGGTGCCGTCCTATCTCAGCTATAAGTTTCTGGGTGAGCGCGATTGTGCCGCGCCCTGCGAGCCCGCAC GGCCCGACGGCTCTATGTTCTTCTCGCAAGAGGAGACTCGTTTTGCCCGTCTCTGGATCCTCACATGGTC GGTGTTGTGCTGCGCTTCCACTTTCTTCACGGTCACCACCTATTTAGTGGACATGCAGCGATTTCGCTAC CCAGAGCGGCCCATCATCTTTCTGTCCGGCTGCTACACCATGGTGTCAGTGGCCTACATTGCGGGCTTCG TTCTCCAGGAGCGCGTGGTATGCAATGAGCGCTTCTCAGAGGACGGTTATCGCACGGTGGTGCAGGGCAC TAAGAAAGAAGGCTGCACTATACTCTTCATGATGCTCTACTTCTTCAGCATGGCCAGCTCCATCTGGTGG GTGATTCTGTCCCTCACCTGGTTCCTGGCAGCCGGAATGAAGTGGGGCCACGAGGCCATCGAGGCCAATT CGCAGTACTTCCACCTGGCCGCCTGGGCCGTGCCGGCCGTCAAAACCATCACCATCTTGGCCATGGGCCA GATCGACGGCGACCTGCTGAGCGGCGTGTGCTTCGTGGGCCTCAATAGCCTGGACCCGCTGCGGGGCTTC GTGCTGGCGCCGCTCTTCGTATACCTGTTCATCGGTACATCCTTCCTGCTGGCCGGCTTCGTGTCACTCT TCCGCATCCGCACCATCATGAAGCACGACGGCACCAAGACGGAGAAGCTGGAGAGGCTCATGGTGCGCAT TGGCGTCTTCTCGGTGCTCTACACGGTACCGGCCACCATCGTCATCGCCTGCTACTTCTATGAGCAGGCC TTCCGCGAGCACTGGGAGCGCTCCTGGGTAAGCCAGCACTGCAAGAGCCTAGCCATCCCCTGCCCGGCCC ACTACACGCCCCGCATGTCGCCCGACTTCACAGTCTACATGATCAAATACCTCATGACGCTCATCGTGGG CATCACGTCGGGCTTCTGGATCTGGTCCGGCAAGACACTGCACTCGTGGAGGAAGTTCTACACTCGTCTC ACCAACAGCCGGCATGGCGAGACCACTGTGTGAAGCGGTCTCGCCTGCCTGCCGGGCTTTCCCCTCTCCC AGGTCCGGACTGCACCGTGCCCTCCTTCACTCAGGAGGGGGGAGGGTGCACCCTACGGACTCCTATTTTA TTTTTTTAAATAAAGAACGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_020510
• Insert Size: 1713 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC055727, AAH55727
• RefSeq Size: 2150 bp
• RefSeq ORF: 1713 bp
• Locus ID: 57265
• UniProt ID: Q9JIP6
• Cytogenetics: 11 E1
• Gene Summary: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.[UniProtKB/Swiss-Prot Function]