Shmt2 (NM_028230) Mouse Untagged Clone

CAT#: MC202532

Shmt2 (untagged) - Mouse serine hydroxymethyltransferase 2 (mitochondrial) (Shmt2), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Shmt2
• Synonyms: 2700043D08Rik; AA408223; AA986903
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC004825 sequence for NM_028230
  GCGTTCTAAGAGCAGCTGGTTTTCGACCCCGGTACTACACCGATACAGAGTTAGTGGCTAGTCCTCCTGT GCCTCCTGTAGCGATGGTATCCTTCTCTTTGCTTCGGACCACTCGGCCTCTGCAGAGATGTGGCCAGCTG GTCTGCATGGCTGCCCGGGCCCAGCACAGCAAGGTGGCCCAGACGCAGGCTGGGGAAGCAACTGGAGGTT GGACGGGCCAGGAGAGTTTATCAGACAGTGACCCTGAGATGTGGGAGCTTCTGCAGAGGGAGAAGGACAG ACAGTGTCGCGGCCTGGAGCTCATCGCCTCAGAGAACTTCTGCAGCCGAGCTGCGCTGGAGGCCCTGGGG TCCTGTCTCAACAACAAGTACTCGGAGGGTTACCCTGGCAAGAGATACTACGGAGGAGCGGAAGTGGTGG ACGAGATCGAGCTGCTCTGCCAGCGCCGGGCCTTGGAAGCCTTTGACCTGGATCCGGCACAGTGGGGAGT CAATGTGCAGCCATACTCAGGGTCCCCAGCCAATCTGGCTGCCTATACGGCCCTTCTGCAGCCTCATGAT CGAATCATGGGGTTGGACCTGCCCGATGGGGGCCATCTCACCCATGGCTACATGTCTGATGTCAAGCGGA TCTCCGCCACATCCATTTTCTTCGAGTCTATGCCCTATAAGCTCAATCCCCAAACTGGCCTCATCGACTA CGACCAGCTGGCGCTGACCGCTCGGCTTTTCCGACCGCGGCTCATCATAGCTGGCACGAGTGCCTATGCC CGCCTCATTGACTATGCACGCATGAAAGAGGTCTGTGATGAGGTCAGGGCACACCTGCTGGCAGACATGG CCCACATCAGTGGCCTGGTGGCTGCCAAGGTGATCCCCTCCCCTTTCAAGTACGCGGATGTTGTTACCAC CACCACTCACAAGACACTGCGAGGGGCCAGGTCAGGGCTCATCTTCTACCGGAAGGGAGTACGAACCGTA GACCCCAAGACTGGCAAAGAGATCCCTTATACCTTTGAGGACCGAATCAACTTCGCTGTGTTCCCATCCC TACAGGGTGGCCCCCACAACCACGCCATTGCTGCAGTAGCCGTGGCTCTCAAGCAGGCCTGCACCCCTAT GTTCCGCGAGTACTCCTTACAAGTGCTGAGGAACGCCCAGGCCATGGCTGATGCCCTGCTCAAGCGAGGA TACTCGCTGGTGTCTGGTGGCACTGACACCCACCTGGTGCTGGTGGACCTGCGGCCCAAGGGCCTGGATG GAGCCCGAGCCGAACGCGTGTTGGAACTTGTCTCCATCACAGCCAACAAGAACACCTGTCCTGGAGACCG GAGCGCCATTACTCCGGGGGGCTTGAGGCTTGGGGCCCCCGCGTTGACCTCTCGCCAGTTCCGTGAGGAC GACTTCCGTAGAGTCGTCGATTTTATCGATGAGGGAGTCAACATTGGCTTGGAGGTGAAGCGCAAGACTG CCAAGCTCCAGGATTTCAAATCCTTCCTGCTCAAAGACCCAGAGACAAGCCAGCGTTTGGCCAACCTCCG GCAACAGGTGGAACAGTTTGCCAGGGGCTTCCCGATGCCTGGATTTGATGAACGTTGAGGACAGTTGAGG ACACCTGGCGGTCGGTCAGAAGCCCACAGACCCGGAAGTTACCTTTCTTCCCGCCTCCCTGGACCATAGA AGAGATGTCACTCCTCTCTGGGTTTTAGTGGAGGGAGGGAGGCCTTCCATTTAGGGCAAGAGCCAGGCAT AAACTCCCTTCCAGAATTTGTAGCTGAGATTTCTGATCTTTAACCGTGTCTACACGAAGCACCCAGTTGA CATCCGACTTGTTTTTGAGACGGGGGTCTCACTTAGCTGTGAGTGGCCTTGAACTGACCCTTCCACCTTC ATTTCCCAAGTGCTGGGGTTATAGGTTTGCACCATGACACCCGACTTCGCCCTCAGCTTTCCACCCCGAC CCTCCCACTACATCTCAAGTGTTACAGACTCCCCTCCCGCTCTTTCTTGGGGAAGAGGGGATGGGTAAGC GCCCTCCTTCCTGTTTTTATCTAATAAAATGCTAACCTGCAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_028230
• Insert Size: 1515 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC004825, AAH04825
• RefSeq Size: 2085 bp
• RefSeq ORF: 1515 bp
• Locus ID: 108037
• UniProt ID: Q9CZN7
• Cytogenetics: 10 D3
• Gene Summary: Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (By similarity). Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate (By similarity). Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (By similarity). Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs (PubMed:29452640). Associates with mitochondrial DNA (By similarity). In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex: required for IFNAR1 deubiquitination by the BRISC complex (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1).