Gskip (NM_178613) Mouse Untagged Clone

CAT#: MC201499

Gskip (untagged) - Mouse RIKEN cDNA 4933433P14 gene (4933433P14Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gskip
• Synonyms: 4933433P14Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC031494 sequence for NM_178613
  GGGGTGGTGCCGGCGCCGCGATCCTGGCCCCAGCAGTCTCCGGACGAGGAAGGATAAGAACATGGGCGCT CGCAGAATGGAAACAGACTATAATCCCGTGGAGCTAAGCAGTATGTCTGGATTTGAAGAAGGCTCCGAGC TCAATGGGTTTGAAGGAGCGGATATGAAGGACATGCAGCTGGAGGCCGAGGCAGTTGTAAACGATGTACT CTTTGCTGTCAACCACATGTTTGTCTCAAAAAGCATGCCCTGTGCAGATGATGTGGCTTACATCAATGTG GAGACAAAGGAAAGGAACAGATACTGCCTGGAGCTCACAGAAGCAGGGCTCAGGGTGGTGGGCTATGCTT TTGACCAGGTGGAGGATCATTTGCAAACCCCCTACCATGAGACAGTCTACTCCTTGTTGGATACTCTCAG CCCTGCCTACCGGGAAGCATTCGGAAACGCGCTCCTTCAGAGACTGGAAGCTTTGAAACGGGATGGACAG TCATGACTCATGACTCATCTTCCTCCTAATGGGGCCGCTGCTGGTATAGAATCTAACATAAGGCTTGACA CCCTTGCGTACAGTCACTGTAGAAAGCGCATCATTCATTGGCTTTGTGTGTTCGCCATTCCTTCACACGC AGGCTTTTGACTCCCAGCGTAATTGAGTTGTCTTAGTTTCAGCTTCATGGAAGGGGATTTAATTTTGAGA CCATTGCTCTGACACTCCCATTCTGAGTTTCACACCCAGCACTCTGCATTTCAATCCCTAGTCAGCTCTT CATTCTCCTGTTACAGGGCTTCAATGCTGCCAGCACTCTTTTACATAGAAAATTCTAGATTTGCACAGTG ATACAGAAATTAGAATCACCTAACTTCAGACCTGGATTCAGCCTGCTAAATCAGGGGTTTACTACTAGCT TGGAGTGACTTTGTAGTGGTTATTTTGTACCTGGCCTTATTGGAAACAAACTGTCAACTAGTTTCCCCTG CACAAATTCTGAAATGTACTGCTCTGCTTAATCTAGTCACATGACCAAGTGGGCTGGTGAAGATGGGTTG CTCATCTGTTTCTTAACTAAGATAAAATGGAAGGCAGGGACAGAAATCGTTCTCCATTCCTTGTTTGCAA CAGCCAGCCAGCCTAGAGCGGACAGACCATTCGCAAATGAATGTAACAATTACTCATCTCCATGATGTCT AAGCACATGAGCAAATAGAGGAACAGGCCCACCTCATCCTCCAGTGTGGATCTCTTCAAAGGAAGAAGAT CCTGGTTTCCTAGGAAACGGTATTTTGGCCTGTGTTGATTCTTACTCTGAATGTTTGTTTACAATGTACA GTATATATATATATATGCACATATATATTCAGAACGTATTTTTGCTTCAATGCTTACTTTCTATAAAGCA GTGCTTTGGTATTCCTACAGCGCCCTTCTTCCCCAGCAGTTGGACAGTGCTCTGTCTCATTGCCACATGA ACAGTGTAACATGAGTGCATTGTATGGTTCGAAACCAAAGGATGAATGAAGCATTCAGAAACTTGATATT TAAAAAAGAGATGCTCTGTATTTTATATTTTATTACAGTCCCCGGTGTTTATAAACGTAATAAAGTCCTC CGTGCTGCTGCATGTTTTCCAGTATGTGACAAACAGAAAGGACCTGGCTCTGTTCTAGTTGTCACCCAGA GCTGCCTCAGCTGCCACCGTCTGCATCTGGGCACACCTTCATACACTGGTCATCAGCAGATGTTTTTGTC GTTAGTTTGCAAAGGGCATTTTTAGCCTCTGAAGACCATGGGAGGAAAGGTGAAGGGTAGAGCCCTTGCC TGTCGTGGGTAAGCACCCAGCCCTGTCCTGAGGATCTAAACAGTCTTAAAATGCTTCTGGGGACCAGGCT TGGTAGAGTTCCCTCTGCATTGCCAATGCCACCGAGAGTTCACTGGGTGTTTGACCCTGGCTTTACAAGA CTCTTTTTCTGGTGTGATGTCTGGTTGCCTGCAGTGTGCACAGTGAAGCCCAGCCTCAGGCTCTCTTTCT GGTGTGATGTCTGGTGTGATGCCTGCAGTGTGCACAGTGAAGCCCAGCCTCAGGCTCTCTTTCTGGTGTG ATTTCTGGTTGCCTGCAGTATGCACAGTGAAGTCCGGCCTCAGGCTCTCTTTCTGGTGTGATGTCTGGTT GCCTGCGGTGTGCACAGTGAAGTCCGGCCTCAGGCTCTCTTTCTGGTGTGATTTCTGGTTGCCTGCAGTG TGCACAGTGAAGCCCGGCCTCAGGCTCTCTTTCTGGTGTGATGTCTGGTGTGATGCCTGCAGTGTGCACA GTGAAGCCCTGCCTCAGGCTCTCTTTCTGGTGTGATGTCTGGTTGCCTGCAGTGTGCACAGTGAAGCCCA GCCTCAGGCTCTCTTTCTGGTGTGATGTCTGGTGTGATGCCTGCAGTGTGCACAGTGAAGCCCAGCCTCA GGCTCTCTTTCTGGTGTGATTTCTGGTTGCCTGTGGGGTGCACAGAGAAGCCCGGCCTCAGGCTCTCTTT CTGGTGTGATGTCTGGTTGCCTGCAGTGTGCACAGTGAAGCCCGGCCTCAGGTTGAACTTTCTTGTGCTT AATCTCAGGCTAACATTTTAAAAAATGACGTTTGTAGAGTTTAAATAAAATTATTTACTTGATTTTGAAA AAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_178613
• Insert Size: 435 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031494, AAH31494
• RefSeq Size: 2678 bp
• RefSeq ORF: 435 bp
• Locus ID: 66787
• UniProt ID: Q8BGR8
• Cytogenetics: 12 E
• Gene Summary: A-kinase anchoring protein for GSK3B and PKA that regulates or facilitates their kinase activity towards their targets. The ternary complex enhances Wnt-induced signaling by facilitating the GSK3B- and PKA-induced phosphorylation of beta-catenin leading to beta-catenin degradation and stabilization respectively. Upon cAMP activation, the ternary complex contributes to neuroprotection against oxidative stress-induced apoptosis by facilitating the PKA-induced phosphorylation of DML1 and PKA-induced inactivation of GSK3B. During neurite outgrowth promotes neuron proliferation; while increases beta-catenin-induced transcriptional activity through GSK3B kinase activity inhibition, reduces N-cadherin level to promote cell cycle progression (By similarity). May play a role in cleft palate formation and is required for postnatal life through modulation of the activity of GSK3B during development (PubMed:26582204).[UniProtKB/Swiss-Prot Function]