Rnf112 (NM_009548) Mouse Untagged Clone

CAT#: MC201184

Rnf112 (untagged) - Mouse ring finger protein 112 (Rnf112), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rnf112
• Synonyms: bfp; neurolastin; Zfp179; ZNF179
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC013139 sequence for NM_009548
  TCTCTGAGCATCGGACCCTCTGCCATTCCAACCTCTCATGCCGAGGCCCGTCCTGTCAGTCACTGCTTTT TGTCATCGGCTTGGCAAACGGGAGAGCAAACGAAGCTTCATGGGAAACAGCAGCAACAGTTGGGTTCTCC CCAGGGAGGAAGCACAAGGCTGGATGGGCCAGGCTGTGCAGGGAGGGACCAGGACAAGCAGGTCCCATGC ATCATTCCCCAAGCTGGAGCTGGGCCTGGGACACCGTCCCTCCCCAACCCGGGAGCCGCCCACCTGCTCC ATCTGTCTGGAAAGGCTTCGAGAGCCCATCTCGCTGGACTGTGGCCATGACTTCTGCATCCGATGCTTCA GCACACACCGCATCCCAGGCTGTGAGCTACCATGCTGTCCTGAATGCCGGAAGATCTGTAAGCAAAGGAA GGGCCTCCGCAGTCTAGGGGAAAGGATGAAACTCCTACCACAGCGGCCACTGCCCCCTGCACTGCAGGAG ACCTGTGCTGTGAGGGCAGAACGTCTGCTGTTGGTACGAATCAATGCCTCCGGAGGCCTCATCCTCAGGA TGGGCGCCATAAACCGTTGCCTGAAGCATCCACTGGCCAGGGACACACCCGTCTGCTTATTGGCTGTCCT GGGAGAGCAGCACTCAGGAAAGTCCTTCCTTTTGGACCACTTGCTCAGTGGCTTACCAAGCCTGGAATCT GGTGACAGCGGCAGGCCCAGAGCAGAGGGGTCTCTGCCTGGGATCAGATGGGGCGCTAACGGCCTCACGA GGGGTATCTGGATGTGGAGTCACCCCTTCCTGCTGGGAAAAGAGGGGAAGAAGGTGGCTGTGTTCTTAGT GGACACGGGGGATGTCATGAGCCCAGAACTGAGCAAGGAGACAAGGGTCAAGCTCTGTGCTCTCACCATG ATGCTCAGTTCCTACCAGATCCTGAATACCTCCCAAGAGCTGAAGGATACCGATCTGGGCTATCTAGAGA TGTTCGTTCACGTGGCTGAGGTGATGGGCAAACATTATGGGATGGTACCAATCCAGCATCTGGATCTCTT AGTCCGAGACTCTTCCCATCATAATAAGTCAGGGCAAGGGCACGTGGGTGACATACTCCAGAAGCTGTCC GGCAAATACCCCAAGGTCCAGGAGCTGCTCCTCGGGAAACGGGCCCGCTGTTACCTCCTCCCTGCTCCTG AGAGGCAGTGGGTAAACAAAGACCAAGCCAGCCCCAGAGGCAACACAGAAGATGACTTCTCCCACCATTT CCGGGCCTACATCTTGGACGTGCTGAGCACAGCCCCTCAGCACGCTAAGAGCCGCTGTCAAGGGTACTGG AGTGAGGGGCGTGCCGTGGCCAGGGGAGACAGACGCCTGCTCACAGGGCAGCAGCTAGCACAGGAGATCA AGAATCTCTCTGGCTGGATGGGGAAGACTGGGCCCAGTTTCAACTCTCCTGATGAGATGGCTGCTCAGCT CCATGATCTGAGGAAAGTGGAAGCCGCCAAGAAGGAGTTCGAGGAGTACGTGAGACAGCAGGACATAGCC ACCAAGCGCATCTTCTCTGCACTACGAGTCCTGCCTGACACGATGAGGAACCTCCTCTCTACCCAGAAGG ATGCCATCTTGGCCCGCCATGGTGTGGCCCTGTTGTGCAAGGAGAGAGAGCAGACCTTGGAGGCCCTGGA AGCCGAGCTGCAGGCAGAAGCCAAGGCCTTCATGGACTCCTACACAATGCGCTTCTGTGGCCACCTGGCC GCGGTAGGGGGCGCTGTAGGTGCTGGGCTCATGGGCCTGGCAGGGGGTGTGGTGGGCGCAGGCATGGCAG CAGCGGCGTTGGCTGCTGAGGCTGGGATGGTAGCAGCTGGGGCCGCGGTGGGGGCCACCGGGGCTGCTGT GGTTGGGGGTGGTGTGGGTGCTGGTCTGGCCGCCACTGTGGGCTGCATGGAGAAAGAGGAAGATGAGAGA GTTCAAGGAGGGGACCGAGAGCCCCTACTCCAGGAGGAATAGCAGCAAGATGTCGAAGCGTGAAATACGT GAAAGGCAGTAGGAAGGGTGCGGACATCAGGGACTCAGAGGGGACCACGTATATACTGGCCTTGTTGAAT GCCCCATGTCAAACACTGGCTGAGCTGGGGGTTTACGGTGGGTCCAGAAACCTGAGGAGGCTTTCTGGCC ACGAGGCTGTACTTGGAGTACAGCGGATAGGGTGCAAGGCCTGTTTCTGGCTCTGGATAATTGCTACTCT CTGGAACCTAGGATCTCCCACCACATAAGGCCCCACCTCCTCTACCCAGTTCCAGTTTATTTCCCTGGAG GGCCTGGGCTGGAAATTTGCCCCTGGATCCAGAACCCCATTCGTTTTACAGTTGGGATAGGTCCCAGCAT AACTGACTTTGGAGCTAGGAGCCCCGCTAACAGGGAAGAAAAAGGAGGGGTCACAGAGCAGGGATGGGGA TGATAGACAGAGCTATAATTCTGGAGTGGTGACTTGTTTCCAAAACTGCTCCAGAAGGCCAGGCTTGGCT GGCCTCCCTGCTCTTCAGTTGGGTGTCCAGATGTGTGTCCTGAACCGCCCTGTACCCCAGCCTCCTGCAG TCCTCCTAAGCCTGTGCCCTGTGCCAAGCCCATTTGGATGGATCAACACAGATTACATGCCAAAGAACCA CACCTCCGATTGCATTCTGTGCTAAAGCAGTCCGTGCCAGGTTCTGCCAAAGTGTGGGAAGGGAAAGTGC AGGAAACGAGCTCCGTCTTTTAACATAAGCTTGAGCTGTGGGGCCTCTGCGCCACACTGTAAGACCCCCT TGATGCAGCCCTCTGAACGCCAAGCCTCCAGAAAGCTTGTGGGCAGGGGGCCTCTCTCGGGGGATGGAGA TTTCTAAAGAGACTTCTGGGCTGTTAAACTATAAAAGATGTTGGGCATATGACCCTTTATTTTATATAAA AATAAAATGTGTGCGTGTGAATGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_009548
• Insert Size: 1965 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC013139, AAH13139
• RefSeq Size: 2998 bp
• RefSeq ORF: 1965 bp
• Locus ID: 22671
• UniProt ID: Q96DY5
• Cytogenetics: 11 37.96 cM
• Gene Summary: E3 ubiquitin-protein ligase that plays an important role in neuronal differentiation, including neurogenesis and gliogenesis, during brain development. During embryonic development initiates neuronal differentiation by inducing cell cycle arrest at the G0/G1 phase through up-regulation of cell-cycle regulatory proteins (PubMed:21566658, PubMed:28684796). Plays a role not only in the fetal period during the development of the nervous system, but also in the adult brain, where it is involved in the maintenance of neural functions and protection of the nervous tissue cells from oxidative stress-induced damage (PubMed:27918959, PubMed:26792191, PubMed:26951452). Exhibits GTPase and E3 ubiquitin-protein ligase activities. Regulates dendritic spine density and synaptic neurotransmission; its ability to hydrolyze GTP is involved in the maintenance of dendritic spine density (PubMed:26212327).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) uses alternate in-frame splice sites in the 5' coding region, compared to variant 3. It encodes isoform 1, which lacks an internal segment and is shorter, compared to isoform 3.