Irak1 (BC004778) Mouse Untagged Clone

CAT#: MC200007

Irak1 (untagged) - Mouse interleukin-1 receptor-associated kinase 1 (cDNA clone MGC:5708 IMAGE:3257579), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Irak1
• Synonyms: IRAK, mPLK
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC004778
  GCGGAGGGCAAGATGGCGGCGGGTCGAGCGCCGCCTCTTCCGCCCGGCCGGGTGTCGCACGAGGCCGGCC TGAAGGGGAAGTGAGTCAGTGTCCGCGGACCCGGCCCGCTAAGGCCCGCGCCCGCCGAGGCCCCGAGAGG CCTCGGCCCGGGGGCGGCGGCGGCGGCCATGGCCGGGGGGCCGGGCCCCGGGGAGCCTGTGGTTCCAGGC GCCCAGCACTTCTTGTACGAGGTGCCACCCTGGGTTATGTGCCGCTTCTACAAAGTGATGGACGCCCTGG AGCCCGCCGACTGGTGCCAGTTCGCGGCCTTGATCGTGCGCGACCAGACAGAGCTGCGGCTGTGCGAGCG CTCCGAGCAGCGCACAGCCAGTGTTCTGTGGCCCTGGATCAACCGCAACGCGCGCGTAGCTGACCTCGTT CACATCCTCACGCACCTGCAGCTGCTGCGTGCGAGGGACATCATCACAGCCTGGCACCCTCCTGCCCCCG TTGTGCCCCCAAGCACCGCTGCCCCAAGGCCCAGCAGCATCTCTGCAGGCTCTGAGGCCGGGGACTGGAG CCCCCGGAAATTGCAGTCCTCTGCCTCCACCTTCCTCTCCCCAGCTTTTCCAGGCTCCCAGACCCATTCT GAGTCAGAGCTCCTCCAGGTTCCACTCCCTGTTTCCCTCGGGCCACCACTACCATCTTCAGCCCCTTCCT CCACCAAGCAGTCAAGCCCAGAGAGCCCAGTGTCTGGCCTCCAAAGAGCCCATCCCTCCCCGTTTTGCTG GCCCTTCTGTGAGATTTCCCAAGGCACCTGCAACTTCTCTGAAGAACTCAGGATTGGAGAGGGTGGTTTT GGATGTGTGTACCGAGCAGTCATGAGAAATACTACATATGCTGTGAAGAGACTGAAGGAGGAAGCTGACC TAGAGTGGACTATGGTGAAACAGAGCTTCTTAACAGAGGTGGAACAGCTATCAAGGTTTCGTCACCCAAA TATCGTAGACTTTGCTGGCTACTGTGCAGAGAGTGGCTTATACTGCCTTGTTTATGGCTTCTTGCCCAAT GGCTCCTTGGAGGATCAGCTCCACCTTCAGACCCAGGCCTGCTCCCCACTTTCCTGGCCTCAACGACTGG ACATTCTTCTGGGCACAGCCCGGGCTATTCAGTTTTTACATCAGGATAGCCCCAGCCTTATCCATGGAGA CATCAAGAGTTCTAACGTGCTTCTGGATGAGAGACTGATGCCCAAGCTGGGAGACTTTGGCCTGGCTCGT TTCAGCCGCTTTGCGGGGGCCAAAGCAAGCCAGAGCAGTACTGTGGCCCGGACTTCCACAGTTCGAGGTA CCCTCGCCTACTTGCCTGAGGAGTACATCAAGACAGGCCGACTGGCTGTGGACACCGATACCTTCAGCTT TGGGGTGGTAATACTGGAGACCCTTGCTGGTCAGAGGGCTGTGAGGACACAAGGTGCAAAGACCAAGTAT TTGAAAGACCTGATTGAAGATGAGGCTGAAGAGGCTGGAGTGACCCTGAAAAGCACCCAGCCTACTCTGT GGGTGGGTGTAGCCACGGATGCTTGGGCTGCTCCAATTGCTGCCCAGATCTATAAGAAGCACCTGGACTC CAGACCTGGGCCCTGCCCACCCCAGTTGGGCCTGGCCCTGGCTCAACTAGCTTGCTGCTGCATGCACCGT CGGGCCAAGAAGAGGCCCCCCATGACCCAGAGGCAAAACTCCCAACATTCCTTGCTTGCCCTTTGGCTCT ATTTGGGGAGGTGCTCTGTGGAACTAAGTGGTAAAGGGCTGCTGCTTTGCTGGATGAGCCTCAGATCCCA GGAACAGGTATACAAGAGACTAGAAGGGCTTCAGGCAGGGCCTCCCTGGGAGCTAGAGGTTGCCGGCCAT GGCTCCCCTTCCCCACAGGAGAACTCCTACATGTCTACCACTGGCAGTGCCCAGAGTGGGGATGAACCAT GGCAGCCTCTAGTAGTGACCACAAGAGCCCCAGCCCAGGCTGCCCAGCAACTCCAGAGAAGTCCCAACCA GCCAGTGGAAAGTGATGAGAGTGTTCCCGGCCTCTCTGCTACCCTGCATTCCTGGCACTTGACTCCAGGT TCCCACCCAAGCCCAGCGTCCTTCAGAGAGGCTAGCTGTACCCAGGGAGGCACTACCAGAGAATCAAGTG TGAGGAGTAGCCCAGGCTTCCAGCCTACAACCATGGAAGGCTCACCCACGGGCAGCTCATCCCTGCTGTC ATCAGAACCACCACAGATCATCATCAACCCAGCCCGACAGAAGATGGTACAAAAGCTGGCTCTTTATGAA GAAGGGGTCTTGGATAGCCTGCAACTGCTGTCATCAGGCTTTTTCCCAGGCTTGGATTTAGAACCTGAAA AGAGCCAGGGACCTGAAGAAAGTGATGAATTCCAGAGCTGATTTGTTCACTCTGACAAATCCCTCAGACT CAGAGGTCAAAGTTCTCATGCTTGGAAGTTCTCATAGTGTGCAAACTCCTCAGTAGCCCAAAGGCATCAG GTGTGGGGGCTCACTCTGGCAAAGGAGAGAGGGCAGTGGACCCTGCTGTTCTCAGAATGTGAACACAGAC AGGGCCAGCCAAAGAACTTGATAGAAGCTGATAAGGCAGGCAATGTGAGGCCAGCATTTGTGAGCAAGGC AAGCAGAGTACCTAAGGAATTGGGTAGTACAAAACAGAAGACTGGGGCTGGTGAGATGGCTCAGTGGGTA TGAGCACCCGATTGCTCTTCCAAAGGTCCGGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCAT CTGTAACAAGATCTGACGCCCTCTTCTGGAGTGTCTGAAGACAGCTACAGTGTACTTATATATAATAAAT AAATCTTTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC004778
• Insert Size: 2154 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC004778, AAH04778
• RefSeq Size: 2893 bp
• RefSeq ORF: 2154 bp
• Locus ID: 16179
• Cytogenetics: X 37.61 cM
• Gene Summary: Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3 (By similarity).[UniProtKB/Swiss-Prot Function]